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Awaad, M. H. H., A. M. Atta, W. A. A. El-Ghany, M. Elmenawey, A.;K. A. A. Hassan, and N. A. A. G. A. Abdelaleem, "Effect of A Specific Combination of Mannan-Oligosaccharides and b-Glucans Extracted From Yeast Cell Wall on the Health Status and Growth Performance of Ochratoxicated Broiler Chickens", Journal of American Science, 2011.
Awaad, A. M., A. A. Abd El-Aziz, and H. Hefny, "A Survey on Big Data and IOT", Solid State Technology, vol. 63, no. 1, pp. 1582–1598, 2020. Abstract
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Awaad, A. S., T. A. Alhamed, D. J. Maitland, G. A. Soliman, N. A. Al-Jaber, and R. Mounerah, "Evaluation of the antimicrobial activity, sub-chronic toxicity and wound healing effect of Cunninghamellaelegans extract and its Isolated Compounds", Life Science Journal, vol. 11, no. 6, 2014. Abstract
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Awaad, M. H. H., A. M. Atta, W. A. A. El-Ghany, M. Elmenawey, K. Ahmed, A. A. Hassan, A. A. Nada, and G. A. Abdelaleem, "Effect of a specific combination of mannan-oligosaccharides and β-glucans extracted from yeast cell wall on the health status and growth performance of ochratoxicated broiler chickens", Journal of American Science, vol. 7, issue 3, pp. 82-96, 2011. Abstract
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Awaad, M. H. H., A. M. Atta, W. A. A. El-Ghany, M. Elmenawey, K. Ahmed, A. A. Hassan, A. A. Nada, and G. A. Abdelaleem., "Effect of a Specific Combination of Mannan-Oligosaccharides and β-Glucans Extracted from Yeast Cell Wall on the Health Status and Growth Performance of Ochratoxicated Broiler Chickens", Journal of American Science, vol. 7(3), pp. 82-96, 2011.
Awaad, A. S., N. A. Al-Jaber, G. A. Soliman, M. R. Al-Outhman, M. E. Zain, J. E. Moses, and R. M. El-Meligy, "New biological activities of Casimiroa edulis leaf extract and isolated compounds.", Phytotherapy research : PTR, vol. 26, issue 3, pp. 452-7, 2012 Mar. Abstract

The phytochemical investigation of Casimiroa edulis Llave et Lex (Rotaceae) afforded four coumarins: umbelliferone (1), esculetin (2), imperatorin (3) and xanthotoxol (4). The identification of these compounds was achieved by using a combination of m.p., UV, EI-mass, ¹H NMR and ¹³C NMR spectroscopy. Essential oil extracts were analysed by GC/MS leading to the identification of 60 components. Sesquiterpene hydrocarbons accounted for the major make up of the oil. Microbiological screenings of the oil and successive plant fractions were performed, showing promising activity against a number of microorganisms with Minimum inhibitory concentrations (MIC) comparable to the standard antibiotics such as chloramphenicol and kanamycin. The plant ethanol extract (400 mg/kg) and the isolated coumarins (60 mg/kg) showed anticoagulant activity. Analyses to determine the activity of the extracts on liver and kidney function were performed, revealing no negative or detrimental effects.

Awaad, M. H. H., M. A. ELMenawey, M. M. Bashandy, H. M. Salem, E. A. Morsy, and T. Gossens, "Heat stress impedance by acidifiers in Broiler chicken", ACTA Scientific Medical Sciences, vol. 2, issue 9, pp. 84-93, 2018. Abstract
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Awaad, M. H. H., A. M. Atta, M. Elmenawey, B. Shalaby, G. A. Abdelaleem, K. Madian, K. Ahmed, D. Marzin, G. Benzoni, and D. K. Iskander, "Effect of Acidifiers on Gastrointestinal Tract Integrity, Zootechnical Performance and Colonization of Clostridium Perfringens and Aerobic Bacteria in Broiler Chickens", Journal of American Science, vol. 7, issue 4, pp. 618-628, 2011. Abstract
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Awaad, A. M., A. A. Abd El-Aziz, and H. Hefny, "A Survey on Big Data and IOT", Solid State Technology, vol. 63, no. 1, pp. 1582–1598, 2020. Abstract
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Awaad, S. S., S. S. Sallam, and A. A. Moawad, "Incidence of Coliforms in Ice Cream Sold in Cairo and Giza Markets", Veterinary Medical Journal , vol. 62, issue 3, pp. 97-102, 2016.
Awaad, M. H. H., W. A. A. El-Ghany, S. A. Nasef, M. S. El-Halawany, F. F. Mohamed, and A. F. Gaber, "Effect of Na-butyrate supplementation on electromicroscopy, virulence gene expression analysis and gut integrity of experimentally induced Salmonella Enteritidis in broiler chickens", Asian Journal of Poultry Science , vol. 10, issue 3, pp. 126-133, 2016. asian_j_poult_sci_dr_awaad_butyrate.pdf
Awaad, A. M., and H. Hefny, "Parallel Implementation of Statistical DBSCAN Algorithm for Spark-based Clustering on Goolgle Cloud Platform", International Journal of Intelligent Engineering & Systems, vol. 16, issue 2, pp. 279-290, 2023.
Awaad, M. H. H., M. E. Hatem, W. A. A. El-Ghany, A. El-Sawy, and A. Fathi, "Certain epidemiological aspects of Aeromonas hydrophila infection in chickens", Journal of American Science, vol. 7, issue 4, pp. 761-770, 2011. j_amer._sci.pdfWebsite
Awaad, A. S., G. A. Soliman, N. A. Aljaber, and T. A. Al-Hamad, Alcoholic extract of fungi of genus Cunninghamella and use thereof, : Google Patents, 2012. Abstract
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Awaad, M. H. H., A. Atta, B. SHALABY, H. B. Gharieb, M. Elmenawey, K. Madian, F. F. Eman, and A. N. Amal, The Efficacy of Na-Butyrate Encapsulated in Palm Fat on Experimentally Induced Necrotic Enteritis and Enumeration of intestinal resident Clostridium perfringens in Broiler Chickens, , Submitted. Abstract

Over more than five decades, cancer remains a national and international health problem regardless of the discovery of several dozens of novel anticancer drugs (natural and synthetic). Accordingly, screening of natural products for promising anti cancer activity is very initiative field of studies in several countries with diversity of gardenia. Breast and cervix cancers are of the most common gynecological female solid tumors that represent major health problem. Herein, we have assessed the cytotoxic characteristics of several molecules of natural origin (cerulinin, chrysin, honikiol, limonin, mevinolin, resveratrol, salicin, retinol, ascorbic acid and calciferol) against two different gynecological breast (MCF-7) and Cervix (HeLa) solid tumor cell lines. After exposure to serial concentrations of the test compounds, SRB-u assay was undertaken and viability assessment was performed via fitting to Emax model to identify the cytotoxicity parameters such as, IC50 and resistant fraction (R-value). Tested compounds showed cytotoxic efficacy against both gynecological solid tumor cell lines (MCF-7 and HeLa) with IC50's ranged from 0.61 to 131.1 μg/ml. In MCF-7 breast cancer cell line; cerulinin, honikiol, mevinolin and calciferol showed the highest potency with IC50 less than 5 μg/ml. Chrysin showed moderate potency with IC50 of 7.27 μg/ml. Ascorbic acid and resveratrol showed the weakest cytotoxic activity with IC50 more than 10 μg/ml. In HeLa cervix cancer cell line; cerulinin and mevinolin showed the highest potency with IC50 less than 5 μg/ml. Chrysin, honikiol, resveratrol and calciferol showed moderate potency with IC50 of ranging from 5-10 μg/ml. Ascorbic acid was the weakest cytotoxic molecule with IC50 more than 10 μg/ml. Limonine, salicin and retinol failed to exert any cytotoxic effect against MCF-7 or HeLa cancer cell lines in-vitro. RT-PCR analysis revealed that the cytotoxicity of these products is multi-factorial and not solely dependent on p53 expression. Impressively, molecules of potent and moderate potency (except chrysin) showed low resistant fraction in both gynecological solid tumor cancer cell lines. In conclusion, our data showed wide range of variable efficacy of several molecules of natural origin against two hormone dependent solid tumor cell lines.

Awaad, M. H. H., A. M. Atta, M. A. ELMenawey, H. B. Gharib, W. A. A. El-Ghany, and A. A. Nada, "The effect of a combination of â (1-3) D-Glucan and Propionibacterium granulosum on productive performance and immune modulation of immunocompromised and non-immunocompromised broiler chickens", Veterinary World, vol. 6, issue 1, pp. 31-38, 2013. Abstract
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Awaad, N., N. Abbas, and A. Farahat, "Dimensional accuracy of three different viscosities of vinylpolysiloxane(VPS) impression material for maxillary complete dentures", Egyptain dental journal, vol. 58, issue 4, pp. 3091-3095, 2012.
Awaad, M. H., Z. A. Sahar, O. A. El-Shazly, M. A. Affify, and S. B. Iman, "Immunomodulatory properties of inactivated propiobacterium granulosum (IM-104). 1- in non immunosuppressed chicken", J.Egypt. Vet. Med. Assoc., vol. 60, issue 7, pp. 137-148, 2000.
Awaad, M. H. H., M. A. ELMenawey, F. F. Mohamed, E. A. Morsy, H. M. Salem, A. A. Nada, A. S. Hafez, E. Chevaux, and V. Demy, "Drug-Independent Control Strategy of Coccidiosis in Broiler Chickens Using Prebiotic, Prebiotic and Symbiotic", Journal of Agriculture and Veterinary Science, vol. 12, issue 3, pp. 22-33, 2019. Abstract
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Awaad, M. H. H., A. Atta, M. A. Ali, S. A. Zoulfekar, A. El-Sawey, H. B. Ghareb, M. Elmenawey, A. A. Nada, and D. Vanessa, "Effect of pediococcus acidilactici supplementation through drinking water on immune response and E. Coli infection in laying hens.", XIVth European Pou;try Conferences Stavagner, Norway, 2014, pp. 483, 2014. Abstract
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Awaad, M. H. H., G. A. Abdel-Alim, K. S. S. Sayed, A. A. Kawkab, A. A. Nada, A. S. Z. Metwalli, and A. N. Alkhalaf, "Immunostimulant effect of essential oils of peppermint and eucalyptus in chickens.", Pak. Vet. J., vol. 30(2), issue 2, pp. 61-66, 2010.
Awaad, M. H. H., M. E. Hatem, W. A. A. El-Ghany, A. El-Sawy, and A. Fathi., "Certain Epidemiological Aspects of AeromonasHydrophila Infection in Chickens", Journal of American Science, vol. 7, issue 4, 2011. AbstractCU-PDF.pdf

Aeromonashydrophila (A. hydrophila) is one of enteric poultry pathogens of public health importance.This work was designed to investigate certain epidemiological aspects of A. hydrophilaincluding its viability, cycleof infection and its pathogenicity to chicks. A gentamicin resistant A. hydrophila strain (GR A. hydrophila strain)was prepared. The results showed that GR A. hydrophila survived in water for 26 days at room temperature and alsoit could be persist in chicken crates, feces, ration, saw dust and straw for 11, 9, 23, 22 and 17 days, respectively. GRA. hydrophila could induce 8.3% embryonic mortality after dipping of the eggs in infected broth culture. Hatchedchicks from GR A. hydrophila infected eggs showed mortalities reaching 13.3 and 1.7 % during 1st and 2nd weekpost hatching, respectively. Survived infected chicks exhibited signs and lesions of omphalitis, enteritis andsepticaemia and depression in heir weight gain. The rate of GR A. hydrophila re-isolation from dead embryosreached 100%, while it was 95.6, 26, 8.7, 4.4, 2.2 and 4.3% from intestine, liver, heart, spleen, kidney and lung,respectively in sacrificed survivors. Fecal shedding of GR A. hydrophila in chicken breeders revealed higherpercentage in orally infected birds than subcutaneously infected ones. Addition of probiotic to the ration of orallyinfected group resulted in lowering the shedding rate. Re-isolation of the organism from egg shells reached 12 % inorally infected breeders compared to 4 % in orally infected probiotic treated birds. Samples taken from reproductiveand internal organs of parent chicken hens were negative for GR A. hydrophila re-isolation. In conclusion; GR A.hydrophila survives for several weeks in contaminated water, ration and litter. The organism may infect birds byoral route and can colonize intestine. GR A. hydrophila is not congenitally transferred as ovary and oviduct do notplay a role in dissemination of A. hydrophila infection. Addition of probiotic to the ration can reduce fecal sheddingrate as well as re-isolation of A. hydrophila from the egg shells.

Awaad, M. H. H., A. M. Atta, M. Elmenawey, B. Shalaby, G. A. Abdelaleem, K. Madian, K. Ahmed, D. Marzin, G. Benzoni, and D. K. Iskander, "Effect of Acidifiers on Gastrointestinal Tract Integrity, Zootechnical Performance and Colonization of Clostridium Perfringens and Aerobic Bacteria in Broiler Chickens", Journal of American Science, vol. 7, issue 4, pp. 618-628, 2011. Abstract
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Awaad, M., M. Elmenawey, M. Afify, S. Zouelfekar, F. Mohammed, A. Samir, and V. Demy, The Impact of High Stocking Density and Saccharomyces cerevisiae boulardii on Productive Performance, Intestinal Microbiota and Gut Integrity of Broiler Chickens, , 2020/01/05, 2019. Abstracthigh_stocking_density_and_saccharomyces_cerevisiae_boulardii_210785.pdf

AB S T RA C T Day-old male Arbor Acres plus broiler chickens (n=1120) were used to study the effect of dietary supplementation of the Probiotic S. c. boulardii on production performance, intestinal microbiota and gut integrity under HSD challenge. Duration of the trial extended from one day of age up to slaughter (42 days). The birds were allotted into 4 groups (1-4). Those of groups 1(NC) and 3 (T1) consisted of 240 birds. While those of groups 2 (PC) and 4(T2) consisted of 320 birds each. All groups ran contemporaneously. The birds of each group assigned into 10 replicates. Those of groups NC and T1 consisted of 10 birds/replicate/m 2 floor area; while those of groups PC and T2 consisted of 13 birds/replicate/m 2 floor area. Chickens of groups T1 and T2 were dietary supplemented with S.c. bululardii; while PC and NC groups were kept as controls. All experimented birds were vaccinated against different diseases according to the vaccination programs usually adopted in Egyptian chicken broiler farms. The used dietary supplement in challenged and unchallenged HSD improved productive performance variables including BW, BWG, FCR. It improved V/C ratio, fecal and cecal LAB counts and GIT integrity. It also Reduced macroscopic and microscopic lesion scores post vVND virus challenge.