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2021
Ahmed, S., H. T. Mohamed, N. El-Husseiny, M. M. El Mahdy, G. Safwat, A. A. Diab, A. A. El-Sherif, M. El-Shinawi, and M. M. Mohamed, "IL-8 secreted by tumor associated macrophages contribute to lapatinib resistance in HER2-positive locally advanced breast cancer via activation of Src/STAT3/ERK1/2-mediated EGFR signaling.", Biochimica et biophysica acta. Molecular cell research, vol. 1868, issue 6, pp. 118995, 2021. Abstract

Locally advanced breast cancer (LABC) is an aggressive disease characterized by late clinical presentation, large tumor size, treatment resistance and low survival rate. Expression of EGFR/HER2 and activation of intracellular tyrosine kinase domains in LABC are associated with poor prognosis. Thus, target therapies such as the anti-receptor tyrosine kinases lapatinib drug have been more developed in the past decade. The response to lapatinib involves the inhibition of RTKs and subsequently signaling molecules such as Src/STAT3/Erk1/2 known also to be activated by the cytokines in the tumor microenvironment (TME). The aim of the present study is to identify the major cytokine that might contribute to lapatinib resistance in EGFR+/HER2+ LABC patients. Indeed, tumor associated macrophages (TAMs) are the main source of cytokines in the TME. Herein, we isolated TAMs from LABC during modified radical mastectomy (MRM). Cytokine profile of TAMs revealed that IL-8 is the most prominent highly secreted cytokine by TAMs of LABC patients. Using in-vitro cell culture model we showed that recombinant IL-8 (50 and 100 ng/mL) at different time intervals interfere with lapatinib action via activation of Src/EGFR and signaling molecules known to be inhibited during treatment. We proposed that to improve LABC patients' response to lapatinib treatment it is preferred to use combined therapy that neutralize or block the action of IL-8.

ali mohamed ali ismail, A. I. Abdelghany, and A. M. A. Elfahl, "Immediate effect of interscapular cupping on blood pressure, oxygen saturation, pulse rate and chest expansion in sedentary smoker students", Journal of Complementary and Integrative Medicine, vol. 18, issue 2, 2021.
Hussein, H. M., A. M. Morsi, and N. A. Abdelraof, "The immediate effect of sustained natural apophyseal glide on postural stability and pain in individuals presenting with flexion-dominant chronic low back pain: A randomized single-blinded placebo-controlled trial", Journal of Back and Musculoskeletal Rehabilitation, vol. 34, issue 6, pp. 1079-1086, 2021.
Al-Ghamdi, A. A., M. S. Al-Ghamdi, A. M. Ahmed, A. S. A. Mohamed, G. H. Shaker, M. J. Ansari, M. A. Dorrah, K. A. Khan, and T. H. Ayaad, "Immune investigation of the honeybee Apis mellifera jemenitica broods: A step toward production of a bee-derived antibiotic against the American foulbrood", Saudi Journal of Biological Sciences, vol. 28, pp. 1528 - 1538, 2021.
Abu‐Elala, N.M., Shaalan, M. A., S. E. ALI, and N. A. Younis, "Immune responses and protective efficacy of diet supplementation with selenium nanoparticles against cadmium toxicity in Oreochromis niloticus", Aquaculture Research. , 2021.
Abu-Elala, N. M., M. Shaalan, S. E. Ali, and N. A. Younis, "Immune responses and protective efficacy of diet supplementation with selenium nanoparticles against cadmium toxicity in Oreochromis niloticus. ", Aquaculture Research (WILEY), vol. 10, pp. 00:1, 2021.
Abu-Elala, N. M., M. Shaalan, S. E. Ali, and N. A. Younis, "Immune responses and protective efficacy of diet supplementation with selenium nanoparticles against cadmium toxicity in Oreochromis niloticus. ", Aquaculture Research (WILEY), vol. 10, pp. 00:1, 2021.
Abdel-Sabour, M. A., M. A. Rohaim, O. J. A. Salman, Samah E Abodalal, F. F. Mohammad, M. S. Madkour, N. A. Abdel-Wanis, and M. Munir, "Immunogenicity and efficacy of a bivalent vaccine against infectious bronchitis virus.", Comparative immunology, microbiology and infectious diseases, vol. 77, pp. 101670, 2021. Abstract

Infectious bronchitis (IB) is a highly contagious viral disease and is responsible for considerable economic losses in the poultry industry, worldwide. To mitigate the IB-associated losses, multiple vaccines are being applied in the sector with variable successes and thus necessitating the development of a potent vaccine to protect against the IB in the poultry. In the present study, we investigated a bivalent live attenuated vaccine consisting of IB virus (IBV) strain H120 (GI-1 lineage) and D274 (GI-12 lineage) to evaluate its protection against heterologous variant of IBV (GI-23 lineage) in chicken. Protection efficacy was evaluated based on the serology, clinical signs, survival rates, tracheal and kidney histopathology and the viral shedding. Results demonstrated that administering live H120 and D274 (named here Classivar®) vaccine in one day-old and 14 days-old provided 100 % protection. We observed a significant increase in the mean antibody titers, reduced virus shedding, and ameliorated histopathology lesions compared to routinely used vaccination regimes. These results revealed that usage of different IBV vaccines combination can successfully ameliorate the clinical outcome and pathology in vaccinated chicks especially after booster vaccination regime using Classivar®. In conclusions, our data indicate that Classivar® vaccine is safe in chicks and may serve as an effective vaccine against the threat posed by commonly circulating IBV strains in the poultry industry.

Park, J. - G., F. S. Oladunni, M. A. Rohaim, J. Whittingham-Dowd, J. Tollitt, M. D. J. Hodges, N. Fathallah, M. B. Assas, W. Alhazmi, A. Almilaibary, et al., "Immunogenicity and protective efficacy of an intranasal live-attenuated vaccine against SARS-CoV-2.", iScience, vol. 24, issue 9, pp. 102941, 2021. Abstract

Global deployment of an effective and safe vaccine is necessary to curtail the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we evaluated a Newcastle disease virus (NDV)-based vectored-vaccine in mice and hamsters for its immunogenicity, safety, and protective efficacy against SARS-CoV-2. Intranasal administration of recombinant (r)NDV-S vaccine expressing spike (S) protein of SARS-CoV-2 to mice induced high levels of SARS-CoV-2-specific neutralizing immunoglobulin A (IgA) and IgG2a antibodies and T-cell-mediated immunity. Hamsters immunized with two doses of vaccine showed complete protection from lung infection, inflammation, and pathological lesions following SARS-CoV-2 challenge. Importantly, administration of two doses of intranasal rNDV-S vaccine significantly reduced the SARS-CoV-2 shedding in nasal turbinate and lungs in hamsters. Collectively, intranasal vaccination has the potential to control infection at the site of inoculation, which should prevent both clinical disease and virus transmission to halt the spread of the COVID-19 pandemic.

Rashad, E., S. Hussein, D. W. Bashir, Z. S. O. Ahmed, and H. El-Habback, "Immunohistochemical Distribution of Immunocompetent Cells in Water Buffalo Spleen (Bubalus bubalis)", Journal of Advanced Veterinary Research, vol. 11, issue 1, pp. 24-35, 2021.
Rashad, E., S. Hussein, D. W. Bashir, Z. S. O. Ahmed, and H. El-Habback, "Immunohistochemical Distribution of Immunocompetent Cells in Water Buffalo Spleen (Bubalus bubalis)", Journal of Advanced Veterinary Research, vol. 11, issue 1, pp. 24-35, 2021. immunohistochemical_distribution_of_immunocompetent_cells_in_water_buffalo_spleen_bubalus_bubalis..pdf
Mansour, K., D. A. Elwi, S. E. Khalifa, and I. HA, "Immunohistochemical Expression of MUC4 in Different Meningioma Subtypes in Comparison to Some Mesenchymal Non-Meningothelial Tumors", Open access Macedonian journal of medical sciences, vol. 9(A), pp. 626-631, 2021. oamjms-9a-626_2.pdf
Bassam, A. M., Y. Raafat, A. M. A. Al-Aziz, and R. R. Mostafa, "Immunohistochemical Expression of “HCG-β” in Colorectal Carcinoma", Open Access Macedonian Journal of Medical Sciences, vol. Vol. 9 No. A (2021): A - Basic Sciences / Pathology, pp. 789-797, 2021.
Hassan, A. S., M. M. Kohil, S. S. E. Sayed, and S. B. Mahmoud, "Immunohistochemical study of perforin and apoptosis stimulation fragment ligand (FasL)in active vitiligo.", Archives of dermatological research, vol. 313, issue 6, pp. 453-460, 2021. Abstract

Several studies demonstrated a major pathological role of melanocyte-specific cytotoxic CD8 T cells in the pathogenesis of vitiligo. It has been suggested that apoptosis, rather than necrosis, is the mechanism of melanocyte depletion in vitiligo. The aim of this study was to evaluate the expression and distribution of perforin and apoptosis stimulation fragment ligand (FasL) in the epidermis and dermis of the perilesional and non-lesional skin of vitiligo patients in comparison to controls, to assess their possible role in mediating apoptosis in vitiligo. Twenty patients with active non-segmental vitiligo and 20 healthy controls were enrolled in the study. Skin biopsies were taken from perilesional and non-lesional skin of patients with vitiligo, as well as covered skin of controls. Immunostaining for perforin and FasL was performed and the quantitative analysis for the expression of perforin and FasL was carried out in the epidermis and dermis of biopsied specimens. Epidermal perforin, dermal perforin, epidermal FasL, dermal FasL were significantly higher in perilesional as well as non-lesional skin than controls. There was a statistically significant positive correlation between epidermal and dermal perforin in perilesional skin. There was a statistically significant positive correlation between epidermal and dermal perforin, as well as epidermal and dermal FasL in non-lesional skin. In conclusion, the significant expression of perforin and FasL in the epidermis and dermis of both perilesional and non-lesional skin of active vitiligo patients suggests the role of cytotoxic granules and apoptotic cell death pathways in the pathogenesis of active vitiligo.

Mansour, Y. A., E. - S. M. M. Mosallam, S. Hussein, E. M. M. Elleithy,, Jouini R, A. S. Mubarak, T. M. Dawoud, R. A. Alsubki, Jwaher H. Alhaji, Hassan A. Hemeg, et al., Immunolocalization of androgen and vitamin D receptors in the epididymis of mature ram (Ovis aries), , 2021.
Madbouly, N., A. E. Amir, A. Abd El Kader, I. Rabee, and A. Farid, "The immunomodulatory activity of secnidazole–nitazoxanide in a murine cryptosporidiosis model", Journal of Medical Microbiology, vol. 70, issue 3, pp. 001327, 2021.
Ezzat, M. I., M. Hassan, M. A. Abdelhalim, A. M. El-Desoky, S. O. Mohamed, and S. M. Ezzat, "Immunomodulatory effect of Noni fruit and its isolates: insights into cell-mediated immune response and inhibition of LPS-induced THP-1 macrophage inflammation", Food & Function, 2021. d0fo03402a.pdf
Ezzat, M. I., M. Hassan, M. A. Abdelhalim, A. M. El-Desoky, S. O. Mohamed, and S. M. Ezzat, "Immunomodulatory effect of Noni fruit and its isolates: insights into cell-mediated immune response and inhibition of LPS-induced THP-1 macrophage inflammation", Food & Function, vol. 12, pp. 3170–3179, 2021.
Kandeel, A., M. Saied, M. A. L. Daly, H. A. N. A. N. DARWISH, and Y. Alsayed, "Impact of 18F-2-fluoro-2-deoxy-D-glucose PET/computerized tomography on the initial staging and changing the management intent in head and neck squamous cell carcinoma", Nuclear Medicine Communications, vol. 42 , issue 2, pp. 216-224, 2021.