Publications

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A
Al-Eraky, D. M., O. M. N. E. Y. A. M. HELMY, Y. M. Ragab, Z. Abdul-Khalek, E. A. El-Seidi, and M. A. Ramadan, "Prevalence of CagA and antimicrobial sensitivity of isolates of patients with gastric cancer in Egypt.", Infectious agents and cancer, vol. 13, pp. 24, 2018. Abstracts13027-018-0198-1.pdf

Background: ) infection has been recognized as a significant threat for gastric cancer. However, studies that investigated the oncogenic factors and antimicrobial resistance of in Egyptian isolates with gastric cancer are rare. The current study aimed to examine: (1) The pattern of antimicrobial resistance of isolates of Egyptian gastric cancer patients, and (2) the prevalence of Cytotoxin-associated gene A (CagA).

Methods: Samples were collected from patients with gastric cancer. Isolation of was performed using Columbia blood agar supplemented with 10% horse blood, and selective supplement of for 3 to 5 days at 37 °C under microaerophilic condition. Isolates were identified by biochemical traits of oxidase, urease and catalase tests. Antimicrobial susceptibility of isolates was examined against five antimicrobial agents using disc diffusion method. After that, extraction of DNA and Polymerase Chain Reaction (PCR) were performed to amplify the target genes.

Results: Twelve samples were collected from six males and six females Egyptian patients with cancer with an age range from 22 to 65 years. These cases are scarce and samples were collected over a period of almost eleven months. All isolates were confirmed as positive through morphology and biochemical tests. The most effective antibiotic found was ciprofloxacin whereas all isolates showed resistance to metronidazole and erythromycin. The target CagA oncogene gene with expected product size was reported and seven (out of twelve) isolates (58%) were identified as CagA positive.

Conclusion: The current study is unique in two main aspects. First, it reported the pattern of antimicrobial susceptibility and prevalence of CagA gene in from Egyptian patients. Second, it exclusively recruited isolates from gastric cancer patients which were confirmed by clinical and laparoscopic examination. The moderately high prevalence of CagA gene in Egyptian cancer patients calls for more vigilance against that oncogene.

E
El-Korany, S. M., O. M. N. E. Y. A. M. HELMY, A. M. El-Halawany, Y. E. Ragab, and H. H. Zedan, "Kojic acid repurposing as a pancreatic lipase inhibitor and the optimization of its production from a local Aspergillus oryzae soil isolate", BMC Biotechnology, vol. 20, issue 52, pp. 1-16, 2020. s12896-020-00644-9.pdf
F
Farrag, H. A., H. Alaa El-Dien M.S, A. M. Hawas, S. A. A. Hagras, and O. M. N. E. Y. A. M. HELMY, "Potential efficacy of garlic lock therapy in combating biofilm and catheter-associated infections; experimental studies on an animal model with focus on toxicological aspects", Saudi Pharmaceutical Journal, vol. 27, pp. 830-840, 2019.
G
Gawad, W. E., O. M. N. E. Y. A. M. HELMY, W. Tawakkol, and A. G. M. Hashem, "Effect of EDTA on biofilm formation and antibiotic susceptibility of multidrug resistant uropathogenic Escherichia coli clinical isolates in Egypt", African Journal of Microbiology Research, vol. 11, issue 38, pp. 1445-1458, 2017. effect_of_edta_on_biofilm_formation_and_antibiotic.pdf
H
Hagras, S. A. A., A. E. - D. M. S. Hosny, O. M. N. E. Y. A. M. HELMY, M. M. Salem-Bekhit, F. Shakeel, and H. A. Farrag, "Effect of sub-inhibitory concentrations of cefepime on biofilm formation by Pseudomonas aeruginosa.", Canadian journal of microbiology, vol. 67, issue 12, pp. 894-901, 2021. Abstract

This study investigated the effect of cefepime at sub-minimum inhibitory concentrations (sub-MICs) on in vitro biofilm formation (BF) by clinical isolates of . The effect of cefepime at sub-MIC levels (½-/ MIC) on in vitro BF by six clinical isolates of was phenotypically assessed following 24 and 48 h of challenge using the tissue culture plate (TCP) assay. Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to observe the change in expression of three biofilm-related genes, namely, a protease-encoding gene (), fimbrial protein-encoding gene (), and alginate-encoding gene (), in a weak biofilm-producing strain of following 24 and 48 h of challenge with sub-MICs of cefepime. The BF morphology in response to cefepime was imaged using scanning electron microscopy (SEM). The TCP assay showed strain-, time-, and concentration-dependent changes in in vitro BF in following challenge with sub-MICs of cefepime, with a profound increase in strains with inherently no or weak biofilm-producing ability. RT-PCR revealed time-dependent upregulation in the expression of the investigated genes following challenge with ½ and ¼ MIC levels, as confirmed by SEM. Cefepime at sub-MICs could upregulate the expression of BF-related genes and enhance BF by clinical isolates.

Hallol, M., O. Helmy, A. - E. Shawky, A. El-Batal, and M. R. O. A. - A. P. L. B. I. I. of by a paramycoides and on Chitosan, "Optimization of Alpha-Amylase Production by a Local Bacillus paramycoides Isolate and Immobilization on Chitosan-Loaded Barium Ferrite Nanoparticles", Fermentation, vol. 8, issue 5, 2022. fermentation-08-00241-v2.pdf
Helmy, O. M., Y. M. Ragab, and M. M. M. Hussein, "Multiplex polymerase chain reaction (PCR) for the detection of diarrheagenic Escherichia coli and Shigella directly from stool ", African Journal of Microbiology Research, vol. 7, issue 34, pp. 4368-4372, 2013.
HELMY, O. M. N. E. Y. A. M., and M. O. N. A. T. KASHEF, "Different phenotypic and molecular mechanisms associated with multidrug resistance in Gram-negative clinical isolates from Egypt", Infection and Drug Resistance, vol. 10, pp. 479–498, 2017. different_phenotypic_and_molecular_mechanisms.pdf
Helmy, O. M., M. M. M. Hussein, F. E. Murad, and H. A. Shoeb, "The preparation of 6x His-tagged granulocyte colony stimulating factor using an improved in vitro expression", African Journal of Biotechnology , vol. 9, issue 50, pp. 8566-8577, 2010.
Helmy, O. M., Y. M. Ragab, and M. M. M. Hussein, "Detection of genus Salmonella and serogroups A, B, C1, D and (Vi) capsular producing strains using multiplex polymerase chain reaction (PCR) method from stool", African Journal of Microbiology Research, vol. 6, issue 47, pp. 7383-7388, 2012.
I
Ibrahim, K. A., O. M. N. E. Y. A. M. HELMY, M. O. N. A. T. KASHEF, T. R. Elkhamissy, and M. A. Ramadan, "Identification of Potential Drug Targets in Helicobacter pylori Using In Silico Subtractive Proteomics Approaches and Their Possible Inhibition through Drug Repurposing", Pathogens, vol. 9, issue 747, pp. 1-21, 2020.
K
M
Mohamed, A. A., H. Mina, A. M. S. Tosson, S. Elfeky, D. Refaey, O. M. N. E. Y. A. M. HELMY, H. E. H. Deeb, A. E. Reweny, and M. Abdel-Ghany, Correlation of Helicobacter Pylori Infection With IL-8 and Recurrence of Abdominal Pain: Our Experience from an Endemic Area, , 2016.
Q
Qadee, S. E., O. Helmy, T. Almorsy, and M. Ramadan, "MALDI-TOF MS BIOTYPER AND POLYMERASE CHAIN REACTION FOR RAPID IDENTIFICATION OF STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA IN NON-STERILE PHARMACEUTICAL PREPARATIONS", International journal of Pharmaceutical sciences and research, vol. 9, issue 9, pp. 3656-3663, 2018. 8-vol.-9-issue-9-sep-2018-ijpsr-ra-9618_1.pdf
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