Qadee, S. E., O. Helmy, T. Almorsy, and M. Ramadan, "MALDI-TOF MS BIOTYPER AND POLYMERASE CHAIN REACTION FOR RAPID IDENTIFICATION OF STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA IN NON-STERILE PHARMACEUTICAL PREPARATIONS", International journal of Pharmaceutical sciences and research, vol. 9, issue 9, pp. 3656-3663, 2018. 8-vol.-9-issue-9-sep-2018-ijpsr-ra-9618_1.pdf
Al-Eraky, D. M., O. M. N. E. Y. A. M. HELMY, Y. M. Ragab, Z. Abdul-Khalek, E. A. El-Seidi, and M. A. Ramadan, "Prevalence of CagA and antimicrobial sensitivity of isolates of patients with gastric cancer in Egypt.", Infectious agents and cancer, vol. 13, pp. 24, 2018. Abstracts13027-018-0198-1.pdf

Background: ) infection has been recognized as a significant threat for gastric cancer. However, studies that investigated the oncogenic factors and antimicrobial resistance of in Egyptian isolates with gastric cancer are rare. The current study aimed to examine: (1) The pattern of antimicrobial resistance of isolates of Egyptian gastric cancer patients, and (2) the prevalence of Cytotoxin-associated gene A (CagA).

Methods: Samples were collected from patients with gastric cancer. Isolation of was performed using Columbia blood agar supplemented with 10% horse blood, and selective supplement of for 3 to 5 days at 37 °C under microaerophilic condition. Isolates were identified by biochemical traits of oxidase, urease and catalase tests. Antimicrobial susceptibility of isolates was examined against five antimicrobial agents using disc diffusion method. After that, extraction of DNA and Polymerase Chain Reaction (PCR) were performed to amplify the target genes.

Results: Twelve samples were collected from six males and six females Egyptian patients with cancer with an age range from 22 to 65 years. These cases are scarce and samples were collected over a period of almost eleven months. All isolates were confirmed as positive through morphology and biochemical tests. The most effective antibiotic found was ciprofloxacin whereas all isolates showed resistance to metronidazole and erythromycin. The target CagA oncogene gene with expected product size was reported and seven (out of twelve) isolates (58%) were identified as CagA positive.

Conclusion: The current study is unique in two main aspects. First, it reported the pattern of antimicrobial susceptibility and prevalence of CagA gene in from Egyptian patients. Second, it exclusively recruited isolates from gastric cancer patients which were confirmed by clinical and laparoscopic examination. The moderately high prevalence of CagA gene in Egyptian cancer patients calls for more vigilance against that oncogene.

Gawad, W. E., O. M. N. E. Y. A. M. HELMY, W. Tawakkol, and A. G. M. Hashem, "Effect of EDTA on biofilm formation and antibiotic susceptibility of multidrug resistant uropathogenic Escherichia coli clinical isolates in Egypt", African Journal of Microbiology Research, vol. 11, issue 38, pp. 1445-1458, 2017. effect_of_edta_on_biofilm_formation_and_antibiotic.pdf
HELMY, O. M. N. E. Y. A. M., and M. O. N. A. T. KASHEF, "Different phenotypic and molecular mechanisms associated with multidrug resistance in Gram-negative clinical isolates from Egypt", Infection and Drug Resistance, vol. 10, pp. 479–498, 2017. different_phenotypic_and_molecular_mechanisms.pdf
Mohamed, A. A., H. Mina, A. M. S. Tosson, S. Elfeky, D. Refaey, O. M. N. E. Y. A. M. HELMY, H. E. H. Deeb, A. E. Reweny, and M. Abdel-Ghany, Correlation of Helicobacter Pylori Infection With IL-8 and Recurrence of Abdominal Pain: Our Experience from an Endemic Area, , 2016.
Helmy, O. M., Y. M. Ragab, and M. M. M. Hussein, "Multiplex polymerase chain reaction (PCR) for the detection of diarrheagenic Escherichia coli and Shigella directly from stool ", African Journal of Microbiology Research, vol. 7, issue 34, pp. 4368-4372, 2013.
Helmy, O. M., Y. M. Ragab, and M. M. M. Hussein, "Detection of genus Salmonella and serogroups A, B, C1, D and (Vi) capsular producing strains using multiplex polymerase chain reaction (PCR) method from stool", African Journal of Microbiology Research, vol. 6, issue 47, pp. 7383-7388, 2012.
Helmy, O. M., M. M. M. Hussein, F. E. Murad, and H. A. Shoeb, "The preparation of 6x His-tagged granulocyte colony stimulating factor using an improved in vitro expression", African Journal of Biotechnology , vol. 9, issue 50, pp. 8566-8577, 2010.
Tourism