Haikal, S. M., N. F. Abdeltawab, L. A. Rashed, T. I. Abd El-Galil, H. A. Elmalt, and M. A. Amin, "Combination Therapy of Mesenchymal Stromal Cells and Interleukin-4 Attenuates Rheumatoid Arthritis in a Collagen-Induced Murine Model.", Cells, vol. 8, issue 8, 2019. Abstract

Rheumatoid arthritis (RA) is a disease of the joints that causes decreased quality of life. Mesenchymal stromal cells (MSCs) have immunosuppressive properties, with possible use in the treatment of RA. Similarly, interleukin (IL)-4 has been shown as a potential RA treatment. However, their combination has not been explored before. Therefore, this study aimed to investigate the effect of a combination therapy of MSCs and IL-4 in the treatment of RA, using a murine collagen-induced arthritis (CIA) model. Arthritis was induced in Balb/c mice by two intradermal injections of type II collagen (CII), at days 0 and 21. CIA mice were randomly assigned to four groups; group I received an intravenous injection of mouse bone marrow-derived MSCs, while group II received an intraperitoneal injection of IL-4. Group III received a combined treatment of MSC and IL-4, while group IV served as a CIA diseased control group receiving phosphate buffer saline (PBS). A fifth group of healthy mice served as the normal healthy control. To assess changes induced by different treatments, levels of RA-associated inflammatory cytokines and biomarkers were measured in the serum, knee joints, and synovial tissue, using ELISA and Real Time-qPCR. Histopathological features of knee joints were analyzed for all groups. Results showed that combined MSC and IL-4 treatment alleviated signs of synovitis in CIA mice, reverting to the values of healthy controls. This was evident by the decrease in the levels of rheumatic factor (RF), C-reactive protein (CRP) and anti-nuclear antibodies (ANA) by 64, 80, and 71%, respectively, compared to the diseased group. Moreover, tumor necrosis factor-alpha (TNF- α) and monocyte chemoattractant protein-1 (MCP-1) levels decreased by 63 and 68%, respectively. Similarly, our gene expression data showed improvement in mice receiving combined therapy compared to other groups receiving single treatment, where cartilage oligomeric matrix protein (Comp), tissue inhibitor metalloproteinase-1 (Timp1), matrix metalloproteinase1 (Mmp-1), and IL-1 receptor (Il-1r) gene expression levels decreased by 75, 70, and 78%, respectively. Collectively, treatment with a combined therapy of MSC and IL-4 might have an efficient therapeutic effect on arthritis. Thus, further studies are needed to assess the potential of different MSC populations in conjugation with IL-4 in the treatment of experimental arthritis.

Taleb, M. H., N. F. Abdeltawab, R. N. Shamma, S. S. Abdelgayed, S. S. Mohamed, M. A. Farag, and M. A. Ramadan, "Origanum vulgare L. Essential Oil as a Potential Anti-Acne Topical Nanoemulsion-In Vitro and In Vivo Study.", Molecules, vol. 23, issue 9, pp. 2164–2179, 2018. researcharticle.pdf
El-Boghdady, N. A., N. F. Abdeltawab, and M. M. Nooh, "Resveratrol and Montelukast Alleviate Paraquat-Induced Hepatic Injury in Mice: Modulation of Oxidative Stress, Inflammation, and Apoptosis.", Oxidative medicine and cellular longevity, vol. 2017, pp. 9396425, 2017. Abstract

Paraquat (PQ) is one of the most used herbicide worldwide. Its cytotoxicity is attributed to reactive radical generation. Resveratrol (Res) and montelukast (MK) have anti-inflammatory and antioxidant properties. The protective effects of Res, MK, or their combination against PQ-induced acute liver injury have not been investigated before. Therefore, we explored the protective potential of Res and/or MK against PQ hepatic toxicity in a mouse model. Mice were randomly assigned to five groups: group I served as the normal control and group II received a single dose of PQ (50 mg/kg, i.p.). Groups III, IV, and V received PQ plus oral Res (5 mg/kg/day), MK (10 mg/kg/day), and Res/MK combination, respectively. Res and/or MK reduced PQ-induced liver injury, evidenced by normalization of serum total protein, ALT, and AST. Res and/or MK significantly reversed PQ-induced oxidative stress markers glutathione and malondialdehyde. Res and/or MK significantly reduced PQ-induced inflammation reflected in TNF-levels. Furthermore, Res and/or MK reversed PQ-induced apoptosis assessed by differential expression of,, and. Histopathologic examination supported the biochemical findings. Although Res and MK displayed antioxidative, anti-inflammatory, and antiapoptotic activities, their combination was not always synergistic.

Pathophysiology

Pathophysiology coures for undergraduate clinical pharmacy students offered by Dept of microbiology and Immunology, Faculty of Pharmacy, Cairo University.

Pathology and Parasitology (401)

Semester: 
Spring

Pathology and Parasitology course, offered by Dept. of Microbiology and Immunology for second year undergraduate students, General Program, Faculty of Pharmacy, Cairo University.


Virology and Mycology 1403

Semester: 
Fall

Diagnostic Virology and Mycology course 

(1403) 

part of Microbiology and Immunology Diploma  offered by department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University.

علم الفيروسات والفطريات التشخيصى

Basic Immunology 2402 - Postgraduate studies

Semester: 
Fall

Basic Immunology course for masters and diploma students of department of microbiology and immunology, Faculty of Pharmacy, Cairo University. Instructor: Nourtan Abdeltawab, PhD Tuesdays 1 - 3 pm 

Zumbrun, E. E., N. F. Abdeltawab, H. A. Bloomfield, T. B. Chance, D. K. Nichols, P. E. Harrison, M. Kotb, and A. Nalca, "Development of a murine model for aerosolized ebolavirus infection using a panel of recombinant inbred mice", Viruses, vol. 4, no. 12: Multidisciplinary Digital Publishing Institute, pp. 3468–3493, 2012. Abstract
n/a