The Protective effect of Ginger and N- Acetyl Cysteine on Ciprofloxacin-Induced Reproductive Toxicity in Male Rats

Citation:
Nashwa, A. - A. A., A. A. Kawkab, and S. M. Mouneir, "The Protective effect of Ginger and N- Acetyl Cysteine on Ciprofloxacin-Induced Reproductive Toxicity in Male Rats ", Journal of American Science, vol. 7, issue 7, pp. 741-752, 2011.

Abstract:

This study was conducted to evaluate the reproductive toxicity induced by ciprofloxacin antibiotic and the protective effect of ginger and/or N-acetyl cysteine. For this purpose, forty- nine rats were divided into 7 groups (7 rats/group). Group (1) was orally given distilled water (solvent of the used drugs) and kept as a control. Group (2) was daily administered ginger at a dose of 100 mg⁄kg.b.wt. by a stomach tube for 65 days (5 days/week). Group (3) was daily administered N-acetyl cysteine (NAC) at a dose of 50 mg⁄kg.b.wt., by a stomach tube for 65 days (5 days/week). Group (4) was orally administered ciprofloxacin (CPX) at a dose of 12.5 mg⁄kg. b.wt. for 65 days (5 days/week). Group (5) was concurrently administered ciprofloxacin (12.5 mg⁄kg. b.wt) with ginger (100 mg⁄kg. b.wt.) by a stomach tube for 65 days (5 days/week). Group (6) was concurrently administered ciprofloxacin (12.5 mg⁄kg.b.wt) with N-acetyl cysteine (50 mg⁄kg.b.wt) by a stomach tube for 65 days. Group (7) was concurrently administered ciprofloxacin (12.5 mg⁄kg.b.wt.) with ginger (100mg⁄kg.b.wt.) and N- acetyl cysteine (50 mg⁄kg.b.wt) by a stomach tube for 65 days. Semen samples were collected at the end of experimental period and were used for sperm functions analysis. Blood samples were collected to separate serum for biochemical and hormonal studies. Testes homogenate was used for oxidative stress biomarkers (lipid peroxidation (TBARS), reduced glutathione (GSH) and DNA fragmentation test). Testes, epididymis and seminal vesicles were collected for histopathological study. The obtained data revealed that CPX promotes reproductive toxicity in rats through generating oxidative damage. It induces an adverse effect on reproductive organs weight, sperm parameters (sperm count, sperm motility and viability), reproductive hormones (testosterone, LH, and FSH) and histological structures. Ginger and/or NAC have an important role in ameliorating reproductive toxicity induced by CPX through restoring the oxidant- antioxidant balance.

Keywords:

Ciprofloxacin; Reproductive Dysfunction; Oxidative stress; Ginger; N-acetyl cysteine; Antioxidant effect ; Rat

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