Effect of Marjoram Oil on the Clinicopathological, Cytogenetic and Histopathological Alterations Induced by Sodium Nitrite Toxicity in Rats

Citation:
Aita, N. A. A., and F. F. Mohammed, "Effect of Marjoram Oil on the Clinicopathological, Cytogenetic and Histopathological Alterations Induced by Sodium Nitrite Toxicity in Rats", Global Veterinaria, vol. 12, issue 5, pp. 606-616, 2014.

Abstract:

The present study aimed to investigate the potential effect of marjoram oil in alleviating hematological, serum biochemical, chromosomal and histopathological alterations induced by sodium nitrite toxicity in rats. For this purpose, forty adult male rats were divided into four groups (10 rats each);Group (1): received distilled water and served as a control, Group (2) was daily administered marjoram oil at a dose of 0.5 ml/kg b.wt.by stomach tube for 2 months, Group (3) was daily administered sodium nitrite at dose of 30 mg/ kg b.wt. by stomach tube for 2 months and Group (4) was concurrently administered sodium nitrite and marjoram oil with similar doses that mentioned before. The obtained results indicated that sodium nitrite possesses a deleterious effect on blood cytology, induce oxidative damage, hepato-renal dysfunction, chromosomal abnormalities and histopathological alterations. The administration of marjoram oil in conjugation with sodium nitrite minimized the hazard effects of sodium nitrite, it improved RBCs count, PCV, Hb concentration and WBCs count. It increased the level of serum total antioxidant capacity (TAC) and diminished the level of serum malondialdehyde (MDA). Moreover, it decreased the elevated activities of AST, ALT and ALP, cholesterol, BUN, creatinine and glucose concentration and elevated the values of total proteins and albumin in comparison to sodium nitrite group. In addition, marjoram oil ameliorated the genotoxic effect of sodium nitrite as it reduced the frequency of chromosomal aberrations and improved the histopathological changes in liver, kidney and spleen in comparison to sodium nitrite intoxicated group.

Key words:

Marjoram Oil Sodium Nitrite Toxicity Chromosomal Aberrations Hepatotoxicity Histopathology

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