Some studies on Mycoplsamosis in Some Animals, Abdou, Nadra-Elwgoud M. I. , Giza, (2002)
Técnicas de biología molecular utilizadas para eldiagnóstico de agentes patógenos de mastitis., El-Sayed, Amr, Hugo Castañeda Vázquez, Awad Walid, Castañeda Vázquez M. A., Wolter W., and Abdou Nadraelwgoud M. I. , La Mastitis Bovina, Mexico, (2019)
Cross-sectional and histopathological studies of Feline Coronavirus infections in stray cats in Kuwait., Abdou, Nadra-Elwgoud M. I., Al-Batel Maha K., Henedi Adawia A., Al-Mutairi Laila Z., Varghese Koshy, and Samy Attia , Veterinaria italiana, Volume 59, Issue 2, (2023) Abstract

Feline Coronavirus (FCoV) is a worldwide viral infection of felids. The disease is usually asymptomatic, but it can cause mild diarrhoea; however, few numbers of cases may develop a severe systemic disease known as feline infectious peritonitis (FIP). This study aims to determine the prevalence of FCoV shedding in the faeces of stray cats in Kuwait and detect antibodies against FCoV in their serum. Histopathological analyses and RT‑PCR were used to prove cases of FIP. A total of 178 cats were examined for the presence of FCoV in their faeces using a rapid immunochromatography (IC) test. Anti‑FCoV Antibody (Anti‑FCoV Ab) was detected in their serum using ELISA. Eleven samples were tested using RT‑PCR to confirm positive cases. The prevalence of FCoV faecal antigen in stray cats was 32.6%. The overall detection rate of Anti‑FCoV Ab in stray cats was 44.9%. Nine cats tested positive using the RT‑PCR test. Six out of those nine were confirmed to be FIP positive through gross and histopathological examination. The characteristic uveitis and discoloration of the irises were seen. The present study is the first report confirming FCoV infection in stray cats in Kuwait. Postmortem and histopathological lesions in cases of FIP were recorded.

Epidemiological and Molecular Study of Cryptosporidium in Preweaned Calves in Kuwait, Qais A. H. Majeed, Maha S. AlAzemi, Al-Sayegh Mohamed M. T., and Abdou Nadra-Elwgoud M. I. , Animals. 2022;12(14):1805. , Volume 12, Issue 14, p.1805-1814, (2022)
Performance of diagnostic assays used to detect Cryptosporidium oocysts in faecal samples of cattle in Kuwait and genotyping of Cryptosporidium species, Nadra-Elwgoud M. I. Abdou, Maha S. AlAzemi, Al-Sayegh Mohamed M. T., and Majeed Qais A. H. , BMC Veterinary Research, Volume 18, p.336-346, (2022)
Some Studies on mycoplasmosis infection in small ruminants using modern diagnostic methods. , Mohamed, Zeinab R., El-Shafey Dina Y., Abdou Nadra-Elwgoud M. I., and ED-Hamed Fadia Abd , Veterinary Medical Journal, Volume 50, Issue 1, p.49-70, (2010)
Infectious Causes of Neonatal Diarrhea in Cattle in Kuwait with Special Reference to Cryptosporidiosis, Majeed, Qais A. H., Al-Batel Maha K., Abdou Nadra-Elwgoud M. I., El-Azazy Osama M. E., Sami Attia M., and El-Said Hassan , Journal of Animal and Veterinary Advances, Volume 10, Issue 17, p.2282 - 2286, (2011) infectious_causes_-neonatal_diarrhea-cattle-kuwait-cryptosporidiosis.pdf
Evaluation of immunochromatography test for detection of four enteropathogens in the feces of sheep and goats in Kuwait., AlAzemi, Maha S., Majeed Qais A. H., Samy Attia, Henedi Adawia A., Youssef Wessam, and Abdou Nadra-Elwgoud M. I. , Open veterinary journal, Volume 11, Issue 3, p.500-507, (2021) Abstract

Background: Diarrhea in newborn small ruminants continues to be the cause of significant financial loss in sheep and goat farms worldwide. Commercial immunochromatographic (IC) assays have been designed and evaluated to be used for the diagnosis of diarrhea in cattle; however, there are no trials to use rapid tests in small ruminants.

Aim: This study was carried out in Kuwait to evaluate the performance of the rapid immunochromatography test (BoviD-4, BioNote, Inc, Korea) for diagnostics of , rotavirus A (RVA), bovine coronavirus (BCoV), and K99 ( K99) in fecal samples of sheep and goats.

Methods: A total of 85 samples were examined using BoviD-4, and the results were compared with that of polymerase chain reaction for , RVA, and BCoV, whereas for K99 it was by isolation and identification as reference tests.

Results: The kappa test agreement results between the BoviD-4 and reference tests were 0.870 (perfect), 0.783 (substantial), 0.728 (substantial), and 0.281 (fair) for the detection of K99, , RVA, and BCoV, respectively. The sensitivity of BoviD-4 kit was 91.2%, 80.0%, 90.0%, and 37.5% and the specificity was 88.2%, 96.0%, 96.4%, and 92.2% for , RVA, K99, and BCoV, respectively.

Conclusion: The Bovid-4 kit can be used as a rapid pen-side test for spp., K99, and RVA in the field. Nonetheless, care must be taken while interpreting the BCoV results of the kit.

Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait., Abdou, Nadra-Elwgoud M. I., Majeed Qais A. H., Saad Ashraf A., Mijatovic-Rustempasic Slavica, Bowen Michael D., and Samy Attia , BMC veterinary research, Volume 17, Issue 1, p.245, (2021) Abstract

BACKGROUND: Group A rotaviruses (RVA) are zoonotic pathogens responsible for acute enteritis in human and neonatal ruminants. This research aimed to determine the prevalence of RVA in ruminants (cattle, sheep, and goats) and investigate the circulating RVA genotypes in these animals in Kuwait. We conducted a cross-sectional study to detect RVA in ruminants, using an immunochromatography test (IC), direct sandwich ELISA test, and real-time RT-PCR (RT-qPCR) assay using fecal samples.

RESULTS: A total of 400 cattle, 334 sheep, and 222 goats were examined. The prevalence of RVA was 5.3, 1.2, and 2.3%, respectively, using IC. The ELISA test detected RVA from 4.3% of cattle, 0.9% of sheep, and 1.8% of goats. There was a significant association between the occurrence of diarrhea and the presence of RVA in bovine fecal samples (p-value = 0.0022), while no statistical association between diarrhea and the presence of RVA in fecal samples of sheep and goats was observed (p-value = 0.7250; p-value = 0.4499, respectively). Twenty-three of the IC-positive samples (17 from cattle, two from sheep, and four from goats) were tested using a RT-qPCR RVA detection assay targeting the NSP3 gene. The results showed that 21 of 23 IC-positive samples tested positive by RT-qPCR. Detection of RVA genotypes revealed that G10P[11] was the predominant strain in cattle (58.8%), followed by G8P[1] (11.7%). One sheep sample was genotyped as G8P[1]. In addition, G6P[1] and G6P[14] were detected in goat samples.

CONCLUSION: The present study revealed that the IC was more sensitive in detecting RVA antigen in fecal samples than the ELISA test. A higher occurrence of RVA infection was observed in cattle than in sheep and goats. This study suggests that RVA might be a risk factor of diarrhea in bovine calves less than 2 weeks old. This research also demonstrates the circulation of RVA in sheep and goat populations in Kuwait. Finally, the G10P[11] RVA genotype was the most prevalent genotype identified from cattle samples.