Evaluation of a Chromogenic Culture Medium Versus Polymerase Chain Reaction for Diagnosis of Clostridium Difficile in Antibiotic Induced Diarrhea

Citation:
Hussein, A. F. A., N. N. Nawar, M. M. A.Haleim, and R. H. Elsherif, Evaluation of a Chromogenic Culture Medium Versus Polymerase Chain Reaction for Diagnosis of Clostridium Difficile in Antibiotic Induced Diarrhea, , Giza, Cairo univ , 2012.

Thesis Type:

Msc

Abstract:

Key words: Antibiotic associated diarrhea-Clostridium Difficile-
Polymerase chain reaction-Chromogenic culture media.
Background: Antibiotic associated diarrhea (AAD) can be a significant
problem resulting in incomplete duration of therapy and development of
microbial resistance and can cause severe complications e.g. electrolyte
imbalances, dehydration, pseudomembranous colitis, toxic megacolon or
even death.
Clostridium Difficile is the leading cause of antibiotic associated diarrhea
in hospitalized patients.
Materials and Methods: In this work we aimed to evaluate
Chromogenic agar versus polymerase chain reaction in diagnosis of
Clostridium Difficile infection.
The study included 100 cases of antibiotic associated diarrhea and 20
completely healthy individuals as control group
Results: The results was that by PCR for cases, 2/100 cases were positive
for toxin B,and one/100 case positive for Binary toxin, no cases were
positive for toxin A, 2/100 cases were positive for tpi gene, for control
group no samples were positive for any toxin or tpi gene.

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