Publications

Export 58 results:
Sort by: Author Title Type [ Year  (Desc)]
2017
Elaish, M., J. M. Ngunjiri, A. Ali, M. Xia, M. Ibrahim, H. Jang, J. Hiremath, S. Dhakal, Y. A. Helmy, and X. Jiang, Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens, , vol. 12, issue 2: Public Library of Science San Francisco, CA USA, pp. e0171174, 2017. Abstract
n/a
Jang, H., Y. K. Jackson, J. B. Daniels, A. Ali, K. I. Kang, M. Elaish, and C. W. Lee, "Seroprevalence of three influenza A viruses (H1N1, H3N2, and H3N8) in pet dogs presented to a veterinary hospital in Ohio", J Vet Sci, vol. 18, no. S1, pp. 291-298, 2017. AbstractWebsite
n/a
Jang, H., Y. K. Jackson, J. B. Daniels, A. Ali, K. -il Kang, M. Elaish, and C. - W. Lee, "Seroprevalence of three influenza A viruses (H1N1, H3N2, and H3N8) in pet dogs presented to a veterinary hospital in Ohio", Journal of veterinary science, vol. 18, issue S1, pp. 291-298, 2017. Abstract
n/a
Elaish, M., J. M. Ngunjiri, A. Ali, M. Xia, M. Ibrahim, H. Jang, J. Hiremath, S. Dhakal, Y. A. Helmy, X. Jiang, et al., "Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens", PLoS One, vol. 12, no. 2, pp. e0171174, 2017. AbstractWebsite
n/a
Elaish, M., J. M. Ngunjiri, A. Ali, M. Xia, M. Ibrahim, H. Jang, J. Hiremath, S. Dhakal, Y. A. Helmy, X. Jiang, et al., "Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens", PLoS One, vol. 12, no. 2, pp. e0171174, 2017. AbstractWebsite
n/a
Elaish, M., J. M. Ngunjiri, A. Ali, M. Xia, M. Ibrahim, H. Jang, J. Hiremath, S. Dhakal, Y. A. Helmy, and X. Jiang, "Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens", PloS one, vol. 12, issue 2: Public Library of Science, 2017. Abstract
n/a
Elaish, M., J. M. Ngunjiri, A. Ali, M. Xia, M. Ibrahim, H. Jang, J. Hiremath, S. Dhakal, Y. A. Helmy, X. Jiang, et al., "Supplementation of inactivated influenza vaccine with norovirus P particle-M2e chimeric vaccine enhances protection against heterologous virus challenge in chickens", PLoS One, vol. 12, no. 2, pp. e0171174, 2017. AbstractWebsite
n/a
2016
Hiremath, J., K. -il Kang, M. Xia, M. Elaish, B. Binjawadagi, K. Ouyang, S. Dhakal, J. Arcos, J. B. Torrelles, and X. Jiang, Entrapment of H1N1 influenza virus derived conserved peptides in PLGA nanoparticles enhances T cell response and vaccine efficacy in pigs, , vol. 11, issue 4: Public Library of Science San Francisco, CA USA, pp. e0151922, 2016. Abstract
n/a
Jang, H., Y. K. Jackson, J. B. Daniels, A. Ali, K. I. Kang, M. Elaish, and C. W. Lee, "Seroprevalence of three influenza A viruses (H1N1, H3N2, and H3N8) in pet dogs presented to veterinary hospital in Ohio", J Vet Sci, 2016. AbstractWebsite

The prevalence of canine H3N8 influenza, and human H1N1 and H3N2 influenza in dogs in Ohio was estimated by conducting serologic tests on 1,082 canine serum samples; risk factors, such as health status and age were examined. The prevalence of human H1N1, H3N2, and canine H3N8 were 4.0%, 2.4% and 2.3%, respectively, and two samples were seropositive to two subtypes (H1N1 and H3N2; H1N1 and canine influenza virus (CIV) H3N8). Dogs with respiratory signs were 5.795 times more likely to be seropositive against human H1N1 virus than healthy dogs (p-value=0.042). The prevalence of human flu infection increased in accordance with the increase in age and varied by the serum collection month. A commercial ELISA used in this study could not detect nucleoprotein specific antibodies from many HI positive sera, which indicates the need for the development and validation of rapid tests for influenza screening in canine population. In summary, we confirmed low exposure of dogs to CIV and human influenza viruses in Ohio. We identified potential risk factors involved for consideration in future investigations. Our findings also support the need for establishment of reliable diagnostic standards for serologic detection of influenza infection in canine species.

Hiremath, J., K. I. Kang, M. Xia, M. Elaish, B. Binjawadagi, K. Ouyang, S. Dhakal, J. Arcos, J. B. Torrelles, X. Jiang, et al., "Entrapment of H1N1 Influenza Virus Derived Conserved Peptides in PLGA Nanoparticles Enhances T Cell Response and Vaccine Efficacy in Pigs", PLoS One, vol. 11, no. 4, pp. e0151922, 2016. AbstractWebsite
n/a
Hiremath, J., K. I. Kang, M. Xia, M. Elaish, B. Binjawadagi, K. Ouyang, S. Dhakal, J. Arcos, J. B. Torrelles, X. Jiang, et al., "Entrapment of H1N1 Influenza Virus Derived Conserved Peptides in PLGA Nanoparticles Enhances T Cell Response and Vaccine Efficacy in Pigs", PLoS One, vol. 11, no. 4, pp. e0151922, 2016. AbstractWebsite
n/a
Hiremath, J., K. -il Kang, M. Xia, M. Elaish, B. Binjawadagi, K. Ouyang, S. Dhakal, J. Arcos, J. B. Torrelles, and X. Jiang, "Entrapment of H1N1 influenza virus derived conserved peptides in PLGA nanoparticles enhances T cell response and vaccine efficacy in pigs", PLoS One, vol. 11, issue 4: Public Library of Science, 2016. Abstract
n/a
2015
Awe, O. O., K. I. Kang, M. Ibrahim, A. Ali, M. Elaish, Y. M. Saif, and C. W. Lee, "Age-Related Susceptibility of Turkeys to Enteric Viruses", Avian Dis, vol. 59, issue 2, pp. 207-12, 2015. AbstractWebsite

Several different enteric viruses have been identified as the causes of gastrointestinal infections in poultry. Enteric virus infections are well characterized in poults, but limited studies have been conducted in older birds. The susceptibility of 2-, 7-, 12-, 30-, and 52-wk-old turkeys to turkey coronavirus (TCoV) and turkey astrovirus (TAstV) was evaluated, as well as the effect of combined infection of TAstV and TCoV in 2-wk-old poults and turkey hens. From cloacal swabs and intestines, TCoV was consistently detected by reverse transcriptase-PCR throughout the experimental period (1-21 days postinoculation [DPI]) from all age groups. In contrast, the last detection point of TAstV gradually decreased to 21, 16, and 12 DPI in birds inoculated at 2, 7, and 12 wk of age, respectively, and viral RNA was rarely detected from cloacal swabs or intestinal contents in turkey hens within 3 DPI. Infection with TAstV alone did not affect body weight in poults or egg production in hens. The combined infection of TAstV and TCoV did not induce more severe clinical signs and pathology than the TCoV infection alone. However, a severe prolonged decrease in egg production (about 50%) was observed in turkey hens in the combined infection group compared with a transient egg production drop in the TCoV-infected hens alone. The underlying mechanism regarding the age-related TAstV susceptibility and the pathogenesis of the TAstV and TCoV coinfection in layer hens needs to be further elucidated.

Awe, O. O., K. -il Kang, M. Ibrahim, A. Ali, M. Elaish, Y. M. Saif, and C. - W. Lee, Age-related susceptibility of turkeys to enteric viruses, , vol. 59, issue 2: American Association of Avian Pathologists 953 College Station Road, Athens …, pp. 207 - 212, 2015. Abstract
n/a
Elaish, M., K. I. Kang, M. Xia, A. Ali, S. A. Shany, L. Wang, X. Jiang, and C. W. Lee, "Immunogenicity and protective efficacy of the norovirus P particle-M2e chimeric vaccine in chickens", Vaccine, vol. 33, issue 38, pp. 4901-9, 2015. AbstractWebsite

The ectodomain of the influenza matrix protein 2 (M2e) is highly conserved across strains and has been shown to be a promising candidate for universal influenza vaccine in the mouse model. In this study, we tested immune response and protective efficacy of a chimeric norovirus P particle containing the avian M2e protein against challenges with three avian influenza (AI) viruses (H5N2, H6N2, H7N2) in chickens. Two-week-old specific pathogen free chickens were vaccinated 3 times with an M2e-P particle (M2e-PP) vaccine via the subcutaneous (SQ) route with oil adjuvant, and transmucosal routes (intranasal, IN; eye drop, ED; microspray, MS) without adjuvant. M2e-PP vaccination via the SQ route induced significant IgG antibody responses which were increased by each booster vaccination. In groups vaccinated via IN, ED or MS, neither IgG nor IgA responses were detected from sera or nasal washes of immunized birds. The M2e-PP vaccination via the SQ route significantly reduced the virus shedding in the trachea and the cloaca for all three challenge viruses. Despite the absence of detectable IgG and IgA responses in birds vaccinated with the M2e-PP via intranasal routes, a similar level of reduction in virus shedding was observed in the IN group compared to the SQ group. Our results supports that the universal vaccine approach using M2e-based vaccine can provide cross-protection against challenge viruses among different HA subtypes although the efficacy of the vaccine should be enhanced further to be practical. Better understanding of the protective immune mechanism will be critical for the development of an M2e-based vaccine in chickens.

Elaish, M., K. I. Kang, M. Xia, A. Ali, S. A. S. Shany, L. Wang, X. Jiang, and C. W. Lee, Immunogenicity and protective efficacy of the norovirus P particle-M2e chimeric vaccine in chickens, , vol. 33, issue 38: Elsevier, pp. 4901 - 4909, 2015. Abstract
n/a
Awe, O. O., K. I. Kang, M. Ibrahim, A. Ali, M. Elaish, Y. M. Saif, and C. W. Lee, "Age-Related Susceptibility of Turkeys to Enteric Viruses", Avian Dis, vol. 59, no. 2, pp. 207-12, 2015. AbstractWebsite
n/a
Awe, O. O., K. -il Kang, M. Ibrahim, A. Ali, M. Elaish, Y. M. Saif, and C. - W. Lee, "Age-related susceptibility of turkeys to enteric viruses", Avian diseases, vol. 59, issue 2: American Association of Avian Pathologists 953 College Station Road, Athens …, pp. 207-212, 2015. Abstract
n/a
Awe, O. O., K. I. Kang, M. Ibrahim, A. Ali, M. Elaish, Y. M. Saif, and C. W. Lee, "Age-Related Susceptibility of Turkeys to Enteric Viruses", Avian Dis, vol. 59, no. 2, pp. 207-12, 2015. AbstractWebsite
n/a
Awe, O. O., K. -il Kang, M. Ibrahim, A. Ali, M. Elaish, Y. M. Saif, and C. - W. Lee, "Age-related susceptibility of turkeys to enteric viruses", Avian diseases, vol. 59, no. 2, pp. 207-212, 2015. Abstract
n/a
Elaish, M., K. I. Kang, M. Xia, A. Ali, S. A. Shany, L. Wang, X. Jiang, and C. W. Lee, "Immunogenicity and protective efficacy of the norovirus P particle-M2e chimeric vaccine in chickens", Vaccine, vol. 33, no. 38, pp. 4901-9, 2015. AbstractWebsite
n/a
Elaish, M., K. I. Kang, M. Xia, A. Ali, S. A. S. Shany, L. Wang, X. Jiang, and C. W. Lee, "Immunogenicity and protective efficacy of the norovirus P particle-M2e chimeric vaccine in chickens", Vaccine, vol. 33, issue 38: Elsevier, pp. 4901-4909, 2015. Abstract
n/a
Elaish, M., K. I. Kang, M. Xia, A. Ali, S. A. S. Shany, L. Wang, X. Jiang, and C. W. Lee, "Immunogenicity and protective efficacy of the norovirus P particle-M2e chimeric vaccine in chickens", Vaccine, vol. 33, no. 38, pp. 4901-4909, 2015. Abstract
n/a