Publications

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1996
El-Abyad, M. S., A. M. Abu-Taleb, and T. Abdel-Mawgood, "Effect of the herbicide pyradur on host cell wall degradation by the sugarbeet pathogens Rhizoctonia solani and Sclerotium rolfsii", Canadian journal of botany, vol. 74, no. 9: NRC Research Press, pp. 1407–1415, 1996. Abstract
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1997
El-Abyad, M. S., A. M. Abu-Taleb, and T. Abdel-Mawgoud, "Response of host cultivar to cell wall-degrading enzymes of the sugarbeet pathogens Rhizoctonia solani Kühn and Sclerotium rolfsii Sacc. under salinity stress", Microbiological research, vol. 152, no. 1: Urban & Fischer, pp. 9–17, 1997. Abstract
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2001
Moussa, T. A. A., and S. M. M. Shanab, "Impact of cyanobacterial toxicity stress on the growth activities of some phytopathogenic Fusarium sp.", Az. J. Microbiol, vol. 53, pp. 267–281, 2001. Abstract
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2002
Moussa, T. A. A., and M. A. Rizk, "Biocontrol of sugarbeet pathogen Fusarium solani (Mart.) Sacc. by Streptomyces aureofaciens", Pakistan Journal of Biological Sciences, vol. 5, no. 5, pp. 556–559, 2002. Abstract
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Shalaby, A. M., M. A. Rizk, and T. A. A. Moussa, "Effect of igran on the rhizosphere mycoflora of vicia faba plants grown in soils infested with orabanche crenata and amended with rhizobium leguminosarum", Pakistan Journal of Biological Sciences, vol. 5, no. 5, pp. 517–520, 2002. Abstract
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Moussa, T. A. A., "Studies on biological control of sugarbeet pathogen Rhizoctonia solani Kühn", Online Journal of Biological Science, vol. 2, pp. 800–804, 2002. Abstract
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2003
Rizk, M. A., and T. A. A. Moussa, "Impact of Gamma Irradiation Stresses I. Response of Gamma-irradiated Sugarbeet Seeds to Infection by Soil-borne Fungal Pathogens", Pakistan Journal of Plant Pathology, vol. 2, no. 1, pp. 28–38, 2003. Abstract
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Moussa, T. A. A., and M. A. Rizk, "Impact of Gamma Irradiation Stresses II. Control of Sugarbeet Pathogens Rhizoctonia solani Kühn and Sclerotium rolfsii Sacc.", Pakistan Journal of Plant Pathology, vol. 2, no. 1, pp. 10–20, 2003. Abstract
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2006
Moussa, T. A. A., G. M. Ahmed, and S. M. S. Abdel-Hmid, "Optimization of Cultural Conditions for Biosurfactant Production from Nocardia amarae", Journal of Applied Sciences Research, vol. 2, no. 11: INSInet Publication, pp. 844–850, 2006. Abstract
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2007
Moussa, T. A. A., and N. A. Tharwat, "Optimization of cellulase and -glucosidase induction by sugarbeet pathogen Sclerotium rolfsii", African Journal of Biotechnology, vol. 6, pp. 1048-1054, 2007. AbstractWebsite

The ability to produce cellulose degrading enzymes by sugarbeet pathogen Sclerotium rolfsii Sacc. in liquid synthetic media with carboxymethy cellulose (CMC) as inducer was studied. Several cultural conditions were examined to assess their effect in optimizing enzymes production. Shaking cultures gave higher yields of cellulases compared with static ones. Aspargine supplement was the best nitrogen source, especially at 3.0 g/l concentration, in promoting enzyme production. Variation of cellulose/xylan ratio in the culture medium showed that cellulose and xylan induced both cellulases synthesis but cellulose being the most effective specific substrate. The influence of different inhibitors on enzymes production by S. rolfsii was also studied. Cyclohexmide and ethidium bromide inhibited protein synthesis by S. rolfsii. Moreover, glucose repressed cellulase synthesis in S. rolfsii.

2008
Moussa, T. A. A., and D. M. I. Ali, "Isolation and Identification of Novel Disaccharide of $ąppa$-L-Rhamnose from Penicillium chrysogenum", World Applied Sciences Journal, vol. 3, no. 3, pp. 476–486, 2008. Abstract
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2009
El-Lathy, A. M., G. E. El-Taweel, M. W. El-Sonosy, F. A. Samhan, and T. A. A. Moussa, "Determination of pathogenic bacteria in wastewater using conventional and PCR techniques", Environmental Biotechnology, vol. 5, no. 2, pp. 73–80, 2009. Abstract
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Yasmin, S., B. Abt, M. Schrettl, T. A. A. Moussa, E. R. Werner, and H. Haas, "The interplay between iron and zinc metabolism in Aspergillus fumigatus", Fungal Genetics and Biology, vol. 46, pp. 707-713, 2009. AbstractWebsite

Zinc plays a critical role in a diverse array of biochemical processes. However, excess of zinc is deleterious to cells. Therefore, cells require finely tuned homeostatic mechanisms to balance uptake and storage of zinc. Here we show that iron starvation affects zinc metabolism by downregulating expression of the plasma membrane zinc importer encoding zrfB and upregulating the putative vacuolar zinc transporter-encoding zrcA in Aspergillus fumigatus. Nevertheless, the zinc content of iron-starved mycelia exceeded that of iron replete mycelia, possibly due to unspecific metal uptake induced by iron starvation. In agreement with increased zinc excess and zinc toxicity during iron starvation, deficiency in siderophore-mediated high-affinity iron uptake caused hypersensitivity to zinc. Moreover, an increase of zinc uptake by conditional overexpression of zrfB was more toxic under iron depleted compared to iron replete conditions. This deregulated zinc uptake under iron starvation caused a decrease in heme production and an increase in protoporphyrin IX accumulation. Furthermore, zinc excess impaired production of the extracellular siderophore triacetylfusarinine C but not the intracellular siderophore ferricrocin. Taken together, these data demonstrate a fine tuned coordination of zinc and iron metabolism in A. fumigatus. (c) 2009 Elsevier Inc. All rights reserved.

Moussa, T. A. A., "Molecular characterization of the phenol oxidase (pox2) gene from the ligninolytic fungus Pleurotus ostreatus", Fems Microbiology Letters, vol. 298, pp. 131-142, 2009. AbstractWebsite

The gene (pox2) encoding a phenol oxidase from Pleurotus ostreatus, a lignin-degrading basidiomycete, was sequenced and the corresponding pox2-cDNA was also synthesized, cloned and sequenced. The isolated gene consisted of 2674 bp, with the coding sequence interrupted by 19 introns and flanked by an upstream region in which the putative metal-responsive elements (MREs) were determined in the promoter region (849 bp), where MRE 1, 2, 3 and 4 were located in positions - 20, - 60, - 236 and - 297. A functional TATA consensus sequence was recognized in position - 85, while CAAT and its inversion consensus sequences were recognized in positions - 284, - 554, - 689 and - 752. The putative GC box consensus sequences were recognized in positions - 181 and - 460, and xenobiotic-responsive elements in positions - 107, - 277 and - 390. The isolation of a second cDNA (pox2-cDNA), the nucleotide sequence of pox2, was found to contain an ORF of 1665 bp capable of coding for a protein of 533 amino acid residues. Northern blot analysis revealed that strong transcriptional induction was observed in the copper-supplemented cultures for the pox2 gene.

2010
Schrettl, M., N. Beckmann, J. Varga, T. Heinekamp, I. D. Jacobsen, C. Jöchl, T. A. Moussa, S. Wang, F. Gsaller, M. Blatzer, et al., "HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus", PLoS pathogens, vol. 6, no. 9: Public Library of Science, pp. e1001124, 2010. Abstract
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Schrettl, M., N. Beckmann, J. Varga, T. Heinekamp, I. D. Jacobsen, C. Joechl, T. A. Moussa, S. Wang, F. Gsaller, M. Blatzer, et al., "HapX-Mediated Adaption to Iron Starvation Is Crucial for Virulence of Aspergillus fumigatus", Plos Pathogens, vol. 6, 2010. AbstractWebsite

Iron is essential for a wide range of cellular processes. Here we show that the bZIP-type regulator HapX is indispensable for the transcriptional remodeling required for adaption to iron starvation in the opportunistic fungal pathogen Aspergillus fumigatus. HapX represses iron-dependent and mitochondrial-localized activities including respiration, TCA cycle, amino acid metabolism, iron-sulfur-cluster and heme biosynthesis. In agreement with the impact on mitochondrial metabolism, HapX-deficiency decreases resistance to tetracycline and increases mitochondrial DNA content. Pathways positively affected by HapX include production of the ribotoxin AspF1 and siderophores, which are known virulence determinants. Iron starvation causes a massive remodeling of the amino acid pool and HapX is essential for the coordination of the production of siderophores and their precursor ornithine. Consistent with HapX-function being limited to iron depleted conditions and A. fumigatus facing iron starvation in the host, HapX-deficiency causes significant attenuation of virulence in a murine model of aspergillosis. Taken together, this study demonstrates that HapX-dependent adaption to conditions of iron starvation is crucial for virulence of A. fumigatus.

Moussa, T. A. A., G. M. Ahmed, and S. M. S. Abdel-Hamid, "Mathematical model for biomass yield and biosurfactant production by Nocardia amarae", New Aspects of Fluid Mechanics, Heat Transfer and Environment, pp. 27-33, 2010. AbstractWebsite

The value of maximum specific growth rate (mu(max) = 0.0177 h(-1)), the maximum growth value (X(max)= 1.82 g l(-1)) The initial biomass concentration was calculated (X(o) = 0.0285 g l(-1)), the maintenance coefficient (K(c) = 0.245 g g(-1)h(-1)), the biomass yield (Y(X/S) = 0.039 g g(-1)) and the product yield (Y(P/S) = 0.0828 g g(-1)) were calculated from the model. The presented model provided a satisfactory representation of the biomass growth; biosurfactant production. The average error of biomass growth and biosurfactant production were 10.2 and 5.7%; respectively. The present model gave a satisfactory representation of the biomass growth; whereas the average percentage error in biosurfactant production through the fermentation process was 5.74% and in microorganism concentration was 10.172%.

2011
Moussa, T. A. A., "Cloning and sequencing of phenol oxidase 1 (pox1) gene from Pleurotus ostreatus", African Journal of Biotechnology, vol. 10, pp. 1299-1308, 2011. AbstractWebsite

The gene (pox1) encoding a phenol oxidase 1 from Pleurotus ostreatus was sequenced and the corresponding pox1-cDNA was also synthesized, cloned and sequenced. The isolated gene is flanked by an upstream region called the promoter (399 bp) prior to the start codon (ATG). The putative metal-responsive elements (MREs) were determined in the promoter region, where MRE 1, 2 and 3 were located in positions -20, -62 and -389, respectively. Functional TATA consensus sequences were recognized in positions -78 and -245, while CAAT consensus sequence was recognized in position -171. The putative GC boxes consensus sequences were recognized in positions -175 and -344, and xenobiotic-responsive elements (XREs) in positions -100 and -270. The pox1-DNA gene consists of 2656 bp, with the coding sequence being interrupted by 19 introns. The nucleotide sequence of cDNA (pox1-cDNA) was found to contain an ORF of 1590 bp capable of coding for a protein of 529 amino acid residues. The signal peptide was predicted to be 23 amino acids in length using SIGNALP 3.0 program. Northern blot analysis revealed that strong transcriptional induction was observed in the copper-supplemented cultures for pox1 gene.

2012
Saker, M. M., T. A. A. Moussa, N. Z. Heikal, A. H. A. A. ELLil, and R. M. H. Abdel-Rahman, "Selection of an efficient in vitro micropropagation and regeneration system for potato (Solanum tuberosum L.) cultivar Desirée", African Journal of Biotechnology, vol. 11, no. 98: Academic Journals, pp. 16388–16404, 2012. Abstract
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Elazzazy, A. M., O. A. Almaghrabi, T. A. A. Moussa, and T. S. Abdelmoneim, "Evaluation of some Plant Growth Promoting Rhizobacteria (PGPR) to Control Pythium aphanidermatum in Cucumber Plants", Life Science Journal-Acta Zhengzhou University Overseas Edition, vol. 9, pp. 3147-3153, 2012. AbstractWebsite
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Abdel-Rahman, T. M. A., M. S. Khalil, T. A. A. Moussa, and S. A. - D. A. Al-Qaysi, "Identification and characterization of Streptomyces alkaliscabies sp nov", Journal of Food Agriculture & Environment, vol. 10, 2012. AbstractWebsite
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Ali, M. M. M., Z. K. Mohamed, J. D. Klena, S. F. Ahmed, T. A. A. Moussa, and K. S. Ghenghesh, "Molecular Characterization of Diarrheagenic Escherichia coli from Libya", American Journal of Tropical Medicine and Hygiene, vol. 86, pp. 866-871, 2012. AbstractWebsite

Diarrheagenic Escherichia coli (DEC) are important enteric pathogens that cause a wide variety of gastrointestinal diseases, particularly in children. Escherichia coli isolates cultured from 243 diarrheal stool samples obtained from Libyan children and 50 water samples were screened by polymerase chain reaction (PCR) for genes characteristic of enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), and enteroinvasive E. coli (EIEC). The DEC were detected in 21 (8.6%) children with diarrhea; 10 (4.1%) cases were identified as EAEC, 3 (1.2%) as EPEC, and 8 (3.3%) were ETEC; EHEC, and EIEC were not detected. All DEC were grouped phylogenetically by PCR with the majority (> 70%) identified as phylogenetic groups A and B1. The EAEC isolates were also tested for eight genes associated with virulence using PCR. Multi-virulence (>= 3 virulence factors) was found in 50% of EAEC isolates. Isolated EAEC possessed different virulence traits and belonged to different phylogenetic groups indicating their heterogeneity.

Moussa, T. A. A., and N. M. Khalil, "Solid-State Fermentation for The Production of Dextran from Saccharomyces cerevisiae and Its Cytotoxic Effects", Life Science Journal-Acta Zhengzhou University Overseas Edition, vol. 9, pp. 2210-2218, 2012. AbstractWebsite
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2013
Moussa, T. A. A., R. H. Elsherif, Y. A. Mohamed, M. E. A. Dawoud, and A. M. AboElAref, "Group B streptococcus colonization of pregnant women: comparative molecular and microbiological diagnosis", Comparative Clinical Pathology, vol. 22: Springer-Verlag London Limited, pp. 1229–1234, 2013. Abstract2013-_comp_clin_pathol.pdf

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