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Moussa, T. A. A., "Cloning and sequencing of phenol oxidase 1 (pox1) gene from Pleurotus ostreatus", African Journal of Biotechnology, vol. 10, pp. 1299-1308, 2011. AbstractWebsite

The gene (pox1) encoding a phenol oxidase 1 from Pleurotus ostreatus was sequenced and the corresponding pox1-cDNA was also synthesized, cloned and sequenced. The isolated gene is flanked by an upstream region called the promoter (399 bp) prior to the start codon (ATG). The putative metal-responsive elements (MREs) were determined in the promoter region, where MRE 1, 2 and 3 were located in positions -20, -62 and -389, respectively. Functional TATA consensus sequences were recognized in positions -78 and -245, while CAAT consensus sequence was recognized in position -171. The putative GC boxes consensus sequences were recognized in positions -175 and -344, and xenobiotic-responsive elements (XREs) in positions -100 and -270. The pox1-DNA gene consists of 2656 bp, with the coding sequence being interrupted by 19 introns. The nucleotide sequence of cDNA (pox1-cDNA) was found to contain an ORF of 1590 bp capable of coding for a protein of 529 amino acid residues. The signal peptide was predicted to be 23 amino acids in length using SIGNALP 3.0 program. Northern blot analysis revealed that strong transcriptional induction was observed in the copper-supplemented cultures for pox1 gene.

Sabry, N. M., and T. A. A. Moussa, Characterization and Structural Properties of Glycam1 Gene of Some Domestic Animals, , vol. 12, issue 1: Medknow Publications, pp. 1006 - 1017, 2021. Abstract
Sabry, N. M., and T. A. A. Moussa, "Characterization and Antimicrobial Efficacy of Bovine Dermcidin-Like Antimicrobial Peptide Gene ‎", International Journal Of Pharmaceutical And Phytopharmacological Research, vol. 10, issue 3, pp. 108 - 117, 2020. Abstract2020-_ijppr.pdfWebsite

Due to the significance of the antimicrobial peptides as innate immune effectors, in this study, a novel bovine antimicrobial peptide and its antimicrobial spectrum were described. RNA isolation from various tissues and RT-PCR were conducted. The DCD-like peptide was synthesized, and its antimicrobial effect was analyzed. The bovine dermcidin-like gene contains 5 exons intermittent by 4 introns. Bovine DCD-like mRNA was 398 bp with ORF size of 336 bp. Bovine DCD-like was expressed in skin and blood. Analysis of amino acid compositions showed that cysteine was repeated six times, which indicates the presence of 3 disulfide bonds that play a role in the peptide stability. Bovine DCD-like had an antimicrobial effect on Enterococcus faecalis, Streptococcus bovis, and  Staphylococcus epidermidis, which was highest at 50 and 100 µg/ml. The effect on Candida albicans and Escherichia coli was slightly low. In Staphylococcus aureus, the activity of bovine DCD-like was affected greatly at pH 4.5 and 5.5. The optimum salt concentrations were 50 and 100 mM with E. coli and all other bacterial strains, respectively. In C. albicans, the activity of bovine DCD-like decreased with increasing pH regardless of the concentration of NaCl. The pH 6.5 of the sweat buffer was optimum for the activity of bovine DCD-like. Finally, it was concluded that the bovine DCD-like gene expressed in skin cells and the DCD-like peptide secreted into the sweat, had high antimicrobial activities against many Gram-positive and -negative strains, as well as yeast-like fungus. 

Moussa, T. A. A., and N. M. Khalil, "Chapter 10 - Extremozymes from extremophilic microorganisms as sources of bioremediation", Microbial Extremozymes: Academic Press, pp. 135 - 146, 2022. Abstract

Extremophiles are a group of organisms growing in a wide range of extreme environmental conditions. The extremophilic microorganisms are diverse and are classified into psychrophiles (− 2°C to 20°C), thermophiles (55–121°C), piezophiles (> 500atm), halophiles (2–5M NaCl or KCl), metallophiles (high concentrations of metals, e.g., copper, zinc, lead, cadmium, and arsenic), alkaliphiles (pH>8), and acidophiles (pH<4) according to the extreme environmental conditions in which they grow and can tolerate. The aims of this chapter are to characterize the extremophilic microorganisms and their physiological and molecular efficiencies in bioremediation processes. Interestingly, the remarkable adaptative abilities of extremophilic microorganisms make them an attractive source of biocatalysts for bioremediation. Bioremediation is an important technology for the cleanup of environmental contaminants. Further attention has also been directed to isolation, identification, and characterization of biocatalysts from extremophilic microorganisms, most of them enzymes named extremozymes, which are well adapted to be active also at extreme conditions. Extremozymes are expected to fill the gap between biological and chemical industrial processes because of the remarkable properties of these enzymes. Even though more than 3000 different enzymes have been identified till now, and many of these were used in industrial and biotechnological applications, the enzyme toolbox at the present is still not enough to present demands. A major cause for this is the fact that many available enzymes do not withstand industrial reaction conditions.

Ahmed, S. A., Z. Khan, X. -wei Wang, T. A. A. Moussa, H. S. Al-Zahrani, O. A. Almaghrabi, D. A. Sutton, S. Ahmad, J. Z. Groenewald, A. Alastruey-Izquierdo, et al., "Chaetomium-like fungi causing opportunistic infections in humans: a possible role for extremotolerance", Fungal Diversity, vol. 76, no. 1: Springer Netherlands, pp. 11–26, 2016. Abstract
Moussaa, T. A. A., M. M. S. Eldinc, and A. Alkaldia, "Cadmium (II) ions removal using dried banana bunch powder: experimental, kinetics, and equilibria", Desal. Water Treat., vol. 226: DESALINATION PUBL 36 WALCOTT VALLEY DRIVE,, HOPKINTON, MA 01748 USA, pp. 263 - 275, 2021. Abstract


Moussa, T. A. A., O. A. Almaghrabi, and T. S. Abdel-Moneim, "Biological control of the wheat root rot caused by Fusarium graminearum using some PGPR strains in Saudi Arabia", Annals of Applied Biology, vol. 163, pp. 72-81, 2013. Abstract2013-_ann_appl_biol.pdfWebsite

The aim of this study was to evaluate the efficacy of selected bacterial strains against the wheat soil-borne pathogen Fusarium graminearum under greenhouse conditions. The most potent isolates were 3 isolates out of 18 isolates, which have numbers 3, 9 and 10 with in vitro inhibition index 42.5%, 41.3% and 46.3% respectively. Isolates 3 and 10 were selected for the following experiments. Isolates 3 and 10 were identified as Bacillus subtilis MAA03 and Pseudomonas fluorescens MAA10, respectively according to International Identification Keys and, confirmed by using Biolog system and 16S rDNA where the strains exhibited more than 99.5% sequence identity. Their close taxonomic relationship was further documented by phenotypic similarities. The using of B. subtilis and P. fluorescens separately or in mixture as biocontrol agent against F. graminearum on wheat significantly increased the final germination percent, the mean daily germination and germination index of wheat cultivar, while the mean germination time was significantly decreased relative to infested control. The final infection percent, the mean daily infection and infection index were decreased significantly, while the mean infection time was significantly increased relative to infested control. The use of P. fluorescens as biocontrol agent was the most efficient than B. subtilis or in mixture and the best treatment was seed coating. The application of B. subtilis and P. fluorescens separately or in combination significantly affected the growth parameters of wheat cultivar Tabuki, the root length was significantly increased in seed coating and seed soaking treatments, while non-significantly decreased in case of soil drench treatment relative to infested control. Shoot length was significantly decreased in case of seed coating treatment relative to infested control. The shoot fresh and dry weights were significantly increased in seed coating and seed soaking treatments relative to infested control. The root fresh and dry weights were significantly increased in seed coating and seed soaking treatments relative to infested control. The number of leaves was significantly increased in all treatments relative to infested control.

Al-Halbosiy, M. M. F., Z. A. Thabit, S. A. - D. A. S.Al-Qaysi|, and T. A. A. Moussa, "Biological Activity of Levan Produced from Rhizospheric Soil Bacterium Brachybacterium phenoliresistens KX139300", Baghdad Science Journal, vol. 15, issue 4, pp. 238-243, 2018. 2018-_baghdad_sci_j.pdf
Moussa, T. A. A., M. S. Khalil, N. M. Gomaa, and R. A. Al-Hazzim, "Biodiversity of Rhizoctonia solani AG3 and AG2-1 associated with potato diseases", Life Science Journal, vol. 11, no. 8, 2014. Abstract2014-_life_sci_j-2.pdf


Baeshen, N. A., J. S. Sabir, M. M. Zainy, M. N. Baeshen, S. E. M. Abo-Aba, T. A. A. Moussa, and H. A. I. Ramadan, "Biodiversity and DNA barcoding of soil fungal flora associated with Rhazya stricta in Saudi Arabia", Bothalia Journal, vol. 44, no. 5, pp. 301–314, 2014. Abstract2014-_bothalia.pdf


Moussa, T. A. A., and M. A. Rizk, "Biocontrol of sugarbeet pathogen Fusarium solani (Mart.) Sacc. by Streptomyces aureofaciens", Pakistan Journal of Biological Sciences, vol. 5, no. 5, pp. 556–559, 2002. Abstract
Rashad, Y. M., and T. A. A. Moussa, "Biocontrol Agents for Fungal Plant Diseases Management", Cottage Industry of Biocontrol Agents and Their Applications: Springer, pp. 337 - 363, 2020. Abstract2020-_chapter.pdf


Al-Qaysi, S. A. S., H. Al-Haideri, S. M. Al-Shimmary, J. M. Abdulhameed, O. I. Alajrawy, M. M. Al-Halbosiy, T. A. A. Moussa, and M. G. farahat, "Bioactive Levan-Type Exopolysaccharide Produced by <i>Pantoea agglomerans</i> ZMR7: Characterization and Optimization for Enhanced Production", Journal of Microbiology and Biotechnology, vol. 31, issue 5: The Korean Society for Microbiology and Biotechnology, pp. 696 - 704, 2021/05/. AbstractWebsite

Levan is an industrially important, functional biopolymer with considerable applications in the food and pharmaceutical fields owing to its safety and biocompatibility. Here, levan-type exopolysaccharide produced by Pantoea agglomerans ZMR7 was purified by cold ethanol precipitation and characterized using TLC, FTIR, 1H, and 13C NMR spectroscopy. The maximum production of levan (28.4 g/l) was achieved when sucrose and ammonium chloride were used as carbon and nitrogen sources, respectively, at 35°C and an initial pH of 8.0. Some biomedical applications of levan like antitumor, antiparasitic, and antioxidant activities were investigated in vitro. The results revealed the ability of levan at different concentrations to decrease the viability of rhabdomyosarcoma and breast cancer cells compared with untreated cancer cells. Levan appeared also to have high antiparasitic activity against the promastigote of Leishmania tropica. Furthermore, levan had strong DPPH radical scavenging (antioxidant) activity. These findings suggest that levan produced by P. agglomerans ZMR7 can serve as a natural biopolymer candidate for the pharmaceutical and medical fields.

Noor, S. O., D. A. Al-Zahrani, R. M. Hussein, M. N. Baeshen, T. A. A. Moussa, S. M. Abo-Aba, A. M. Al-Hejin, N. A. Baeshen, and J. P. Huelsenbeck, "Assessment of fungal diversity in soil rhizosphere associated with Rhazya stricta and some desert plants using metagenomics", Arch. Microbiol., 2020, 2021. Abstract2020-_arch_microbiol.pdfWebsite

This study aimed to compare the fungal rhizosphere communities of Rhazya stricta, Enneapogon desvauxii, Citrullus colocynthis, Senna italica, and Zygophyllum simplex, and the gut mycobiota of Poekilocerus bufonius (Orthoptera, Pyrgomorphidae, “Usherhopper”). A total of 164,485 fungal reads were observed from the five plant rhizospheres and Usherhopper gut. The highest reads were in S. italica rhizosphere (29,883 reads). Species richness in the P. bufonius gut was the highest among the six samples. Ascomycota was dominant in all samples, with the highest reads in E. desvauxii (26,734 reads) rhizosphere. Sordariomycetes and Dothideomycetes were the dominant classes detected with the highest abundance in C. colocynthis and E. desvauxii rhizospheres. Aspergillus and Ceratobasidium were the most abundant genera in the R. stricta rhizosphere, Fusarium and Penicillium in the E. desvauxii rhizosphere and P. bufonius gut, Ceratobasidium and Myrothecium in the C. colocynthis rhizosphere, Aspergillus and Fusarium in the S. italica rhizosphere, and Cochliobolus in the Z. simplex rhizosphere. Aspergillus terreus was the most abundant species in the R. stricta and S. italica rhizospheres, Fusarium sp. in E. desvauxii rhizosphere, Ceratobasidium sp. in C. colocynthis rhizosphere, Cochliobolus sp. in Z. simplex rhizosphere, and Penicillium sp. in P. bufonius gut. The phylogenetic results revealed the unclassified species were related closely to Ascomycota and the species in E. desvauxii, S. italica and Z. simplex rhizospheres were closely related, where the species in the P. bufonius gut, were closely related to the species in the R. stricta, and C. colocynthis rhizospheres.

Nascimento, M. M. F., L. Selbmann, S. Sharifynia, A. M. S. Al-Hatmi, H. Voglmayr, V. A. Vicente, S. Deng, A. Kargl, T. A. A. Moussa, H. S. Al-Zahrani, et al., "Arthrocladium, an unexpected human opportunist in Trichomeriaceae (Chaetothyriales)", Fungal biology: Elsevier, 2015. Abstract
Ouf, S. A., T. A. Moussa, A. M. Abd-Elmegeed, and S. R. Eltahlawy, "Anti-fungal potential of ozone against some dermatophytes", brazilian journal of microbiology, vol. 47, issue 3: SBM, pp. 697-702, 2016. Abstract