FEASIBILITY OF A RAPID LATERAL FLOW TEST FOR SIMULTANEOUS DETECTION OF GIARDIA LAMBLIA AND CRYPTOSPORIDIUM PARVUM IN DUODENAL ASPIRATES OF PATIENTS SUFFERING FROM CHRONIC LIVER DISEASES AND ELIGIBLE FOR UPPER ENDOSCOPY

Citation:
ELMALLAWANY, M. A., M. A. M. Ismail, S. S. Attia, R. Ahmed, M. N. Alkady, S. Elkholy, A. Shaker, D. O. HELMY, and R. K. NAHNOUSH, "FEASIBILITY OF A RAPID LATERAL FLOW TEST FOR SIMULTANEOUS DETECTION OF GIARDIA LAMBLIA AND CRYPTOSPORIDIUM PARVUM IN DUODENAL ASPIRATES OF PATIENTS SUFFERING FROM CHRONIC LIVER DISEASES AND ELIGIBLE FOR UPPER ENDOSCOPY ", J. Egypt. Soc. Parasitol. (JESP), vol. 51, issue 2, pp. 305 - 312, 2021.

Abstract:

Chronic liver diseases (CLDs) represent an important health issue in developing countries and
are commonly associated with impaired immunity. This increases the susceptibility to various in-
fectious agents including parasitic infections, which should be properly managed to avoid life
threatening complications. This study assessed the feasibility of rapid, easy and applicable
screening test for Giardia and Cryptosporidium within 150 CLDs patients suitable for upper en- doscopic examination. Stool samples, duodenal aspirates and duodenal biopsies were examined
for G. lamblia and C. parvum by different diagnostic techniques. The results showed stool mi-
croscopy (13.3% & 7.3%), duodenal aspirate microscopy (5.3% & 4.7%), rapid lateral flow im- mune-chromatographic assay (RLFIA) applied on duodenal aspirate samples (16.7% & 10%), duodenal biopsies histopathological examination (6.7% & 5.3%) and direct fluorescent antigen detection in stools (16.7% & 9.3%) for giardiasis and cryptosporidiosis respectively. The high
sensitivity of lateral flow immune-chromatographic assay in detecting Giardia and Cryptospor-
idium in duodenal fluid samples proved a good screening test for these patients.
Key words: Egypt, Chronic liver disease patients, Giardia, Cryptosporidium, Immunofluocen-
ce, duodenal aspirate, rapid immune-chromatography.

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