Publications

Export 61 results:
Sort by: Author Title Type [ Year  (Asc)]
1997
Ismail, M. A. M., and A.I.Younis, "Competition of the larval stages of Fasciola gigantica and Haematoloechus species in the snail intermediate host", The 18 Annual Congress of the Egyptian Society of Parasitology, Kasr Al-Ainy School of Medicne, Cairo University, 6 March, 1997.
1999
Ismail, M. A. M., A.Fathy, and R.ElHarezy, "Clinical trial of fluoquinolones in treatment of acute intestinal amoebiasis", The Egyptian Journal of Medical Sciences, vol. 20, June-December, 1999.
Ismail, M. A. M., G.A.Ahmed, S.O.EL-Basiouni, M.M.Ali, and O.A.Khalafalla, "Diagnosis of recent experimental toxocariasis canis by immunoglobulin G avidity enzyme linked immunosorbent assay ( IgG avidity ELISA )", The Egyptian Journal of Medical Sciences, vol. 20, June-December, 1999.
Orok, W. M., S.N.AbdelSalam, and M.A.M.Ismail, "Effect of topical fluconazole in treating Acanthamoeba keratitis in rabbits", Egyptian Society of Ophthalmology Journal, vol. 92, 1999.
Ismail, M. A. M., M.M.Ali, and O.A.Khalafalla, "In vitro cultivation of Babesia bovis", The Egyptian Journal of Medical Sciences, vol. 20, June-December , 1999.
2000
Ali, M. M., and M.A.M.Ismail, "The use of colchicine in treatment of experimental toxoplasmosis", Journal of the Egyptian Society of Parasitology, vol. 30, April, 2000.
Ali, M. M., S.O.EL-Basiouni, G.A.Ahmed, M.A.M.Ismail, and M. A. A. Raoof, "Detection of Trichomonas vaginalis in pregnant women with preterm labour", The Second Annual Congress, Faculty of Medicine, Faium University, 13-14 April, 2000.
Ismail, M. A. M., M.M.Ali, and A.H.Essa, "Intracellular adhesion molecule-2 in experimental fascioliasis : role in pathogenicity and assessment of course and infection", The 21 Annual Congress of the Egyptian Society of Medical Sciences, Kasr Al-Ainy School of Medicne, Cairo University, 4 May, 2000.
2001
Essa, A. H., M.A.M.Ismail, and A.I.Younis, "Diagnosis of malaria in humans by an immunochromatographic test using monoclonal antibodies", The Egyptian Journal of Medical Sciences, vol. 22, June-December , 2001.
EL-Basiouni, S. O., M.A.M.Ismail, A.H.Essa, G.A.Ahmed, S.Alwakeel, and O.A.Khalafalla, "Eosinophilic Cationic Protien (ECP) and circulating antigen in urine of schistosomiasis haematobium patients: Role in diagnosis and/ or morbidity assessment", The Egyptian Journal of Medical Sciences, vol. 22, June- December , 2001.
2002
Ismail, M. A. M., and O.A.Khalafalla, "Diagnosis of Strongyloides stercoralis infection in immunocompromised patients with malabsorption using coproantigen detection", The Egyptian Journal of Medical Sciences , vol. 23, June-December , 2002.
MORSY, T. A., W.A.Saleh, and M.A.M.Ismail, "The Red fox,Vulpes V. Aegyptiaca, a new host of Leishmania major in Sinai peninsula", Journal of the Egyptian Society of Parasitology, vol. 32, December, 2002.
2003
Essa, A. H., M.A.M.Ismail, and H.El-Mofty, "Anti-Acanthamoeba activity of some contact lens solution constituents available in Egypt", Kasr Al-Ainy Medical Journal, vol. 9, May, 2003.
2004
Moneer, A., A.Salah-Elden, H.El-Mofty, M.A.M.Ismail, and M.Alnabarawy, "The effectiveness of ketoconazole and honey on Acanthamoeba in vitro and experimental keratitis in rabbits", Journal of the Egyptian Society of Cataract and Refractive Surgery, vol. 10, February, 2004.
2005
Ahmed, G. A., S.O.EL-Basiouni, M.A.M.Ismail, A.I.Younis, and O.A.Khalafalla, "Markers of glomerular and/or tubular kidney affection in asymptomatic patients with bancroftian filariasis", Kasr Al-Ainy Medical Journal, vol. 11, September, 2005.
EL-Basiouni, S. O., G.A.Ahmed, A.I.Younis, M.A.M.Ismail, A.N.Saadawy, and S.EL-Basiouni, "A study on Demodex folliculorum mite density and immune response in patients with facial dermatosis", Journal of the Egyptian Society of Parasitology, vol. 35, December , 2005.
Ismail, M. A. M., and O.A.Kalafalla, "Toxocara canis and chronic urticarial in Egyptian patients", Journal of the Egyptian Society of Parasitology, vol. 35, December , 2005.
2006
Kamel, M. M., K.M.Maher, I.Rabia, A.H.Helmy, A.I.EL-Adawi, M.A.Mousa, and A.M.Mahgoub, "A CON A-PURIFIED HYDATID GLYCOPROTEIN FRACTION EFFECTIVELY DIAGNOSES HUMAN HYDATIDOSIS", Journal of the Egyptian Society of Parasitology, vol. 36, issue 3, pp. 945-958, 2006.
2009
El-Awady, A. A., A.M.A.Mahgoub, N.M.Naguib, and M.A.M.Ismail, "Comparative study on the Diagnostic Value of A Recently introduced quantitative chemiluminescence ,a Semi Quantitative ELISA and an IHA Titration in Toxoplasma Gondii Serology", Kasr Al-Ainy Medical Journal, vol. 15, No,12, December, pp. 11-16, 2009. Abstract

Background: Recently, the IMMULITE 200(1 automated, chemiluminescent Toxoplasma quantitative IgM and IgG test, has been introduced, which measures loxo IgM and IgG in International Units per milliliter (lU/ntl) of seiunu Hus method is simplet relatively inexpensive, and rapid Objective: Tin IMMULITE Toxoplasma IgM and IgG assays (Diagnostic Products Corporation, Los Angeles) were compared to two serological reactions, Material add methods: In this study, 162 serum samples .were collected from women in their first trimester of pregnancy and tested for the presence of IgM and IgG antibodies raised against T. gondii infection using IHA, ELISA and Diagnostic Products Cot partition (DEG) hnmulite systems,
Results: In relation to sensitivity and specificity, IMMULITE presented the highest (W0%) value for the detection of both IgM and IgG. ELISA showed 100% sensitivity and 97.6% specificity for the detection of IgM and 88.9% sensitivity and 95.4% specificity for the detection of IgG IHA had a sensitivity of 37.5% and 88.9% and a specificity of 100%. and 98.7% for the detection of IgM and IgG respectively*
Conclusion: These results indicate that the IMMULITE Toxoplasma sensitive and specific toutsfor the detection ofIgM and IgG antibodies to Toxoplasma gondii

2011
Ismail, M. A. M., "Evaluation of serological and molecular techniques in clinical stage of toxoplasmosis", The Egyptian Journal of Medical Sciences, 2011.
Ismail, M. A. M., M.M.A.Basyouni, W.M.El-Komy, and S.Shaker, "Modulatory role of immunization with secretory excretory products of Schistosoma haematobium eggs on morbidity in infected hamsters", Parasitologists United Journal, vol. 4, No.2, December, pp. 201-210 , 2011. Abstract

The present study was designed to investigate different parasitological parameters in response to injection of purified secretory - excretory products (SEP) of S. haematobium eggs into hamsters prior to infection with cercariae as an experimental trial for decreasing or modulating severe morbidity

Kobtan, H., and M.A.M.Ismail, "Therapeutic Efficacy of topical fluconazole monotherapy in the management of contact lens related Acanthamoeba keratitis : a Preliminary Report on 3 Cases", Journal of the Egyptian Society of Cataract and Refractive Surgery, vol. 17, February , pp. 39-42 , 2011. Abstract

Purpose: To report the therapeutic efficacy of topical Fluconazole on three cases of culture proven Acanthamoeba keratitis. Materials and methods: Prospective case series. Three eyes of 3 patients with culture-proven AK were treated at our tertiary care institution at Cairo University.Topical Fluconazole 2% was instituted as an alternative treatment for resistant infectious keratitis that proved as culture positive for Acanthamoeba. Successful treatment was defined by absence of clinical signs of active infection and visual
improvement. trophozoites was seen migrating on nonnutrient agar overlain with E. coli.
Results: The first case showed a central large full thickness corneal abscess 8 mm in diameter with melting and the second case showed a small paracentral superficial corneal infiltrate with radial keratoneuritis. The third case presented with a central descmetocele and surrounded by a corneal ring abscess. Acanthamoeba Castellanii was cultured from the Contact lens case in all 3 patients. Topical fluconazole 2% eye drop was used for a duration ranging from 4weeks up to 2 month as a single modality. None of the three had recurrent disease following discontinuation of the drug over a period of follow up ranging from two months up to 4 year.
Conclusion: Topical Fluconazole could be considered in cases of Acanthamoeba keratitis as an alternative therapy to a combination of polyhexamethylene biguanide (PHMB) and propamidine isethionate (Brolene). However further in vivo studies and randomized clinical trials are needed to compare relative efficacy of both modalities.

2015
Ismail, M. A. M., N.F.A.Imam, S. S. A. Gawad, and S.S.El-Wakil, "GIARDIA LAMBLIA IN DYSPEPTIC DIARRHEIC PATIENTS ATTENDING OCTOBER 6 UNIVERSITY HOSPITAL, EGYPT", The Egyptian Journal of Medical Sciences, vol. 36, issue 1, pp. 383 - 392, June, 2015. Abstract

Giardiasis is a major cosmopolitan protozoan illness which usually presents with diarrhea among other manifestations. Dyspepsia is a very frequent gastrointestinal symptom that may be associated with giardiasis, and is giardiasis is often overlooked as a cause or contributing factor in the development of dyspepsia in non-peptic ulcer patients. In this cross-sectional study, 80 diarrheic adult patients complaining of dyspepsia as a main symptom were enrolled, along with 40 control subjects. The study patients had a mean age of 45.23 ± 11.27, and were 42 (53%) males and 38 (47%) females. By concentration technique and direct microscopy, Giardia lamblia (G. lamblia) trophozoites were detected in 9 (11.25%) cases. Results of coproantigen detection by ELISA and a rapid dipstick immunochromatography test were superior, detecting 13 (16.25%) and 15 (18.75%) patients, respectively. This was statistically significant compared to both results of microscopy and healthy controls, who were all G. lamblia negative by all used techniques. In conclusion, G. lamblia appears to have a role in the development or persistence of dyspepsia patients. It should be ruled out or treated, especially in patients not responding to dyspepsia treatment. Moreover, rapid coproantigen detection dipstick tests are highly recommended as a diagnostic tool, especially in the absence of experienced microscopists and if only one stool sample is available.

2016
Abdel Aal, S. M., J.A.Ahmed, M.A.M.Ismail, and S. Z. A. Maogood, "The antigenic relationship between Schistosoma mansoni and its intermediate snail host", J.Egypt.Soc.Parasitol.(JESP), vol. 46 No(3), pp. 663-670, December, 2016. Abstract

Schistosomiasis is a public health problem in many developing countries including Egypt,
Determination of the antigenic relationship between S. mansoni and its intermediate snail host
(IMH) Biomphalaria alexandrina can open a new field for diagnosis and control of the dis-
ease. In the present study infected and non-infected B. alexandrina foot and visceral hump tis-
sue as well as S. mansion crude Ag (SWAg) were fractionated using SDS-PAGE. It's specific
and cross reacted protein fractions were determine using EITB versus experimentally prepared
mice hyper immune sera (HIS) versus each antigen.
After treatment of fractionated S.mansoni crude worm antigens (SWAg) versus HIS produced
after vaccination of mice by the same Ag, 8 kda protein fractions ranged from 35-140 kda
were reacted specifically. Treatment of fractionated B.alexandrina infected and non-infected
foot and visceral hump Ag versus previous HIS revealed presence of common polypeptides
bands between SWAg and non-infected snail antigens. The fraction at 135 kda, 68 kda, were
detected in all cases, while that at 40-42 kda and that at 35 kda was diagnosed in SWAg and
that of infected snails only. The fraction at 68 kda was reacted specifically between SWAg and
all tested fractionated snail antigens either that of foot or visceral hump when they treated sep-
arately by HIS of mice vaccinated by each snail Ag separately. The fraction at 135 kda was
common between SWAg and snail (infected and non-infected) visceral hump antigen. The
fraction at the level of 110 kda was diagnosed in SWAg, in non-infected foot antigen and vis-
ceral hump Ag. The fraction at the level of 46-48 kda are common between SWAg and snail
foot and visceral hump Ag after treated by HIS of mice vaccinated by foot Ag,
Presence of common antigenic fractions between snail tissues and Schistosoma species can
prefer an easily source of antigen valuable for diagnosis or vaccination as well as can be con-
sidered as new tool for determination to the snail IMH of new discovered trematode parasites.
Key words: S.mansoni- B.alexandrina- EITB- Common fractions.

Aly, I. R., M.A.M.Ismail, D. M. H. E. Akkad, H.El-Sayed, E.S.Taher, M.El-Dardiry, and H. M. El-Askary, "Application of competitive ELISA for detection of Fasciola gigantica tegument-associated antigen in human fascioliasis", International Journal of Biology, Pharmacy and Allied Sciences ( IJHPAS ), issue 2277-4998, October, 2016. Abstract

Tropical fascioliasis is considered to cause a significant economic loss in livestock industry in developing and underdeveloped countries. The aim of the current study was to evaluate the diagnostic capacity of Fasciola gigantica (F. gigantica) tegument-associated antigen to be compared with Kato-Katz method in diagnosis of human fascioliasis. The F. gigantica tegument-associated antigen was purified from the crude extracts by Superose-12 HR-10/300 column gel filtration chromatography. Polyclonal antibody (pAb) against purified F. gigantica tegument-associated antigen was prepared by immunization of rabbits with F. gigantica tegument-associated antigen. 83 blood and stool samples were collected from Fasciola patients, healthy control, and patients with other parasites. Detection of circulating F. gigantica tegument-associated antigen in human serum and stool samples by competitive enzyme linked immunosorbant assay (ELISA) was done. The results revealed that the sensitivity and specificity of competitive ELISA for detection of Fasciola antigen in sera was 86.2% and 92.6 % in comparison to the sensitivity and specificity of competitive ELISA for detection of Fasciola antigen in stool samples which gave 96.3% and 96.3%, respectively. These results indicated the high sensitivity and specificity of competitive ELISA for immunodiagnosis of fascioliasis in both serum and stool samples using pAbs against F. gigantica tegument-associated antigen.
Key words: Fasciola gigantica, tegument-associated antigen, serum, stool, indirect ELISA, Kato Katz method

Tourism