Molecular characterization of genetic biodiversity in ducks, using RAPD-PCR analysis

Citation:
E.A.El-Gendy, M.Helal, N.H.Goher, and A. Mostageer, "Molecular characterization of genetic biodiversity in ducks, using RAPD-PCR analysis", Arab Journal of Biotechnology, vol. 8, issue 2, pp. 253-264, 2005. copy at www.tinyurl.com/zrsv7pr

Abstract:

ABSTRACT This study aimed at elucidating the genetic variation within and between duck populations and estimating the phylogenetic relationships among them. Five duck breeds of two species, Muscovy and Sudani of Cairina species, and White Pekin, Damietti and Khaki Campbell of Anas species, were used. The technique of random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied using five random primers. Individual DNA samples within breed and also a bulk of DNA samples for each breed were used. Primers amplified bands with an overall mean of 8.45 and 8.68 bands for individual and pooled samples, respectively. No significant difference was observed between pooled and individual samples indicating the reliability of mixing DNA samples within breed. High levels of polymorphism and heterozygosity between breeds were obtained. Two primers (OPC-08 and OPC-11), resulted in highly polymorphic markers for duck genomes. Specific bands were obtained for species and also for breeds. The overall mean of genetic variability indices within breed was low to moderate and averaged 0.38, whereas band sharing levels within breed were high and averaged 0.73. Variability estimates between breeds were 0.47 and 0.35 for individual and pooled samples respectively, whereas band sharing estimates were 0.63 and 0.74. Genetic distance indices between breeds ranged between 0.264 and 0.728.

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