A model for the genetic employment of chickens local to warm climate. 2. Genome scanning of two lines selected for growth

Citation:
El-Gendy, E. A., E. H. Abbas, S. M. El-Tantawy, A. T. Mohamed, M. Helal, Z. M. M. El-Deen, and A. Y. Wageh, "A model for the genetic employment of chickens local to warm climate. 2. Genome scanning of two lines selected for growth", African Journal of biological sciences, vol. 9, pp. 95-105, 2013. copy at www.tinyurl.com/nl7q7ar

Abstract:

The genome scanning was performed on four chicken populations to estimate the genomic
changes associated with genetic selection. The populations were the local naked-neck line CE3 that
has been selected for 6-wk BW for 6 generations with its genetic control line CE4, and a broiler
dam line D with its genetic control line CD. In lines CE3 and D. The PCR was applied to pooled
DNA samples of each line and sex using seven microsatellite markers. The average number of
alleles per microsatellite locus was 2.14, denoting to the multi-allelic property of the detected loci.
The total number of allelic bands and their frequencies differed among lines and sexes,
demonstrating the genetic composition specificity of each line and the sexual dimorphism. The
variability, heterozygosity and PIC averaged 0.54, 0.68 and 0.81 in line CE3 versus 0.46, 0.52 and
0.57 in line CE4. The moderate variability and moderately high heterozygosity estimates in line
CE3 indicate that there is still much work can be done through selection. The distance between line
CE3 and line CE4 averaged 2.92, and the distance between line D and its control line CD averaged
1.95. These distances reveal the progress of selection practiced in lines CE3 and D, that has been
expressed in shifting the genetic composition of them from those of their control lines CE4 and CD.
The longer distance between line CE3 and line CE4 than that between line D and line CD indicates
the higher selection progress achieved in line CE3 compared to line D.

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