Genetic variation and polymorphism at microsateliite loci in chickens of worm regions selected for meat production.

Citation:
El-Gendy, E. A., and M.Helal, "Genetic variation and polymorphism at microsateliite loci in chickens of worm regions selected for meat production.", International Journal of Biotechnology and allied fields, vol. 2, pp. 100-116, 2014. copy at www.tinyurl.com/o6epptx

Abstract:

In a research project aiming at the genetic improvement of warm-region originated chickens,
a breeding program was practiced on naturally heat-resistant local population in Egypt and
four lines have been derived. The lines were the homozygous normally-feathered selected
(CE1) and control (CE2) lines, and the homozygous naked-neck selected (CE3) and control
(CE4) lines. Lines CE1 and CE3 have been selected for 6-wk body weight for eight
generations. The objective of this study was to assess the genetic features of the selected lines
at the microsatellite loci recognized by 27 microsatellite primers in generations 6, 7 and 8.
The number of alleles detected by each primer varied from one to seven. The number of
alleles per primer and generation averaged 5.72, 2.35, 5.98 and 2.57 in lines CE1, CE2, CE3
and CE4, respectively. The genetic variability was in general low among lines and ranged
0.17–0.20. Polymorphic information content (PIC) averaged 0.50, .39, 0.55 and 0.50 in lines
CE1, CE2, CE3 and CE4 respectively, and line-specific alleles (LSA) formed 4.3, 0.7, 4.6
and 1.2% of total alleles in corresponding lines. The differences between each selected line
and its control line in PIC and LSA were significant, except between line CE3 and line CE4
in PIC. The results denoted to the possible linkages between the detected microsatellite loci
and QTL for body weight. By generation 8, the genetic distance indices between line CE1
and line CE2 averaged 0.740, and between line CE3 and line CE4 averaged 0.815. The
phylogenetic dendograms revealed the genetic progress of the selected lines over subsequent
generations.

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