Pathology of parasitic diseases

Semester: 
Spring
Shaalan, M., M. El-Mahdy, S. Theiner, N. Dinhopl, M. El-Matbouli, and M. Saleh, "Silver nanoparticles: Their role as antibacterial agent against Aeromonas salmonicida subsp. salmonicida in rainbow trout (Oncorhynchus mykiss).", Research in veterinary science, vol. 119, pp. 196-204, 2018 Aug. Abstractas5308044513116161503565051679_content_1.pdf

The rise of bacterial resistance to antibiotics is one of the great challenges of our age. One of the strategies to limit the development of antibiotics resistance is the investigation of alternative antimicrobials. As silver nanoparticles demonstrated a potent bactericidal activity in vitro, the aim of this study was to evaluate the in vivo antibacterial activity of silver nanoparticles against Aeromonas salmonicida subsp. salmonicida. Rainbow trout (n = 120) were divided into four groups of 30 fish each. First group was challenged with A. salmonicida (Positive control), the second group was challenged with A. salmonicida and exposed to silver nanoparticles by immersion for three hours (100 μg/L), the third group was challenged with A. salmonicida and intraperitoneally injected with silver nanoparticles (17 μg/mL) and the fourth group was sham-treated and served as a negative control group. At the 7th day post challenge, histopathology of the positive control group revealed the presence of bacterial aggregates in tissues with degenerative and necrotic changes, while at the 35th day post challenge, only liver necrosis persisted. Silver nanoparticles-treated and negative control groups did not show any clinical signs, mortalities or histopathological alterations and they were tested negative for A. salmonicida. The immersion in silver nanoparticles did not result in detectable residues of silver in the muscles 35 days after treatment. These findings demonstrate the antibacterial properties of silver nanoparticles against A. salmonicida infection. Therefore, they could be used for development of antibacterial agents in aquaculture.

Shaalan, M., M. El-Mahdy, M. Saleh, and M. El-Matbouli, "Aquaculture in Egypt: Insights on the Current Trends and Future Perspectives for Sustainable Development", Reviews in Fisheries Science & Aquaculture , vol. 26, issue 1, pp. 99-110, 2018. aquacultureinegyptinsightsonthecurrenttrendsandfutureperspectivesforsustainabledevelopment.pdf
Shaalan, M. I., M. M. El-Mahdy, S. Theiner, M. El-Matbouli, and M. Saleh, "In vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens", Acta Veterinaria Scandinavica, vol. 59, pp. 49, 1970 Aug 21, 2017.
Shaalan, M., M. Saleh, M. El-Mahdy, and M. El-Matbouli, "Recent progress in applications of nanoparticles in fish medicine: A review", Nanomedicine: Nanotechnology, Biology and Medicine , vol. 12, issue 3, pp. 701-710, 2016. Abstract

Nanotechnology has become an extensive field of research due to the unique properties of nanoparticles, which enable novel applications. Nanoparticles have found their way into many applications in the field of medicine, including diagnostics, vaccination, drug and gene delivery. In this review, we focused on the antimicrobial effects of nanoparticles, with particular emphasis on the problem of antibiotic resistant bacteria in fisheries. The use of nanoparticle-based vaccines against many viral pathogens is a developing field in fish medicine research. Nanoparticles have gained much interest as a specific and sensitive tool for diagnosis of bacterial, fungal and viral diseases in aquaculture. Nevertheless our review also highlights the many applications of nanotechnology that are still to be explored in fish medicine.

Abdelsalam, M., M. Y. Elgendy, M. Shaalan, M. Moustafa, and M. Fujino, "Rapid identification of pathogenic streptococci isolated from moribund red tilapia (Oreochromis spp.).", Acta veterinaria Hungarica, vol. 65, issue 1, pp. 50-59, 2017 03. Abstract

Accurate and rapid identification of bacterial pathogens of fish is essential for the effective treatment and speedy control of infections. Massive mortalities in market-sized red tilapia (Oreochromis spp.) were noticed in mariculture concrete ponds in northern Egypt. Histopathological examination revealed marked congestion in the central vein of the liver with the presence of bacterial aggregates inside the lumen and in the vicinity of the central vein. A total of 12 isolates of streptococci were obtained from the moribund fish. This study documented the ability of the MicroSeq 500 16S bacterial sequencing method to accurately identify Streptococcus agalactiae and S. dysgalactiae mixed infections from moribund red tilapia that were difficult to be recognised by the commercial biochemical systems. The continuously decreasing cost of the sequencing technique should encourage its application in routine diagnostic procedures.

Adl, A., I. B. Shaheed, M. I. Shaalan, A. K. Al-Mokaddem, and A. E. Hassanien, "Digital Pathological Services Capability Framework", Advanced Machine Learning Technologies and Applications: Springer International Publishing, 2014.
Mahdy, M. M. E., T. A. S. E. Din, H. S. Aly, F. F. Mohammed, and M. I. Shaalan, "Evaluation of hepatotoxic and genotoxic potential of silver nanoparticles in albino rats", Experimental and Toxicologic Pathology, vol. 67, issue 1, pp. 21-29, 2015.
Moftah, H., M. I. Shaalan, A. E. Hassanien, and G. Schaefer, Mammary Gland Tumor Detection in Cats using Ant Colony Optimisation, , 2013.
Shaalan, M. I., "Over view on silver nanoparticles: Applications versus toxicity", The 24th congress of Egyptian society of animal reproduction and fertility, Hurghada, Egypt, 2014.
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