Essential oil of cultivated in Egypt: seasonal variation in chemical composition and anticholinesterase activity.

Citation:
Essential oil of cultivated in Egypt: seasonal variation in chemical composition and anticholinesterase activity., Madi, Yasmine F., Choucry Mouchira A., Meselhy Meselhy R., and El-Kashoury El-Sayeda A. , Natural product research, p.1-5, (2020)

Abstract:

The yield of essential oil obtained by hydrodistillation of fresh leaves ranged from 0.15% to 0.46% w/w; being the highest in spring and the lowest in winter. The oil sample obtained in winter exhibited a moderate acetylcholinesterase inhibitory activity (IC 2.86 ± 0.17 mg/mL), compared to physostigmine (IC 0.012 mg/mL), while other samples were relatively weak (IC values of 2.86-5.40 mg/mL). In all samples, oxygenated monoterpenes were predominating (73.22-89.32%). GC-MS identified a total of 61, 25, 50 and 63 components in oil samples obtained in spring, summer, autumn and winter, respectively. Citral content was the highest in autumn and summer samples (82.02% and 80.01% citral; respectively) and the lowest in winter sample (60.01%). Citral, isolated from the oil demonstrated a relatively potent anticholinesterase activity (IC 0.21 ± 0.01 mg/mL).