Cytotoxicity of shikonin metabolites with biotransformation of human intestinal bacteria,
MIN, Byung-Sun, Meselhy Meselhy-R, Hattori Masao, Kim Hwan-Mook, and Kim Young-Ho
, Journal of microbiology and biotechnology, Volume 10, Issue 4, p.514-517, (2000)
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Human intestinal Bacteroides spp. RHEIN-I and RHEIN-II capable of transforming rhein to rheinanthrone, induce rhein-dependent diarrhea in rats,
Meselhy, Meselhy Ragab, Nishimoto Eri, Akao Teruaki, and Hattori Masao
, J Trad Med, Volume 18, Issue 4, p.169-176, (2001)
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Shikometabolins A, B, C and D, novel dimeric naphthoquinone metabolites obtained from shikonin by human intestinal bacteria,
Meselhy, Meselhy R., KADOTA Shigetoshi, Tsubono Koji, KUSAI Akihiko, Hattori Masao, and NAMBA Tsuneo
, Tetrahedron letters, Volume 35, Issue 4, p.583-586, (1994)
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Developing of anti-HIV agents from natural resources (Chemical & Pharmacological study),
Meselhy, Meselhy Ragab, El-Mekkawy Sahar, Ma Chaomei, Nakamura Norio, TEWTRAKUL Supinya, and Hattori Masao
, 和漢医薬学雑誌, Volume 22, Issue 1, p.116-128, (2005)
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Two new quinochalcone yellow pigments from Carthamus tinctorius and Ca2+ antagonistic activity of tinctormine,
Meselhy, Meselhy R., KADOTA Shigetoshi, Momose Yasunori, HATAKEYAMA Noboru, KUSAI Akihiko, Hattori Masao, and NAMBA Tsuneo
, Chemical and pharmaceutical bulletin, Volume 41, Issue 10, p.1796-1802, (1993)
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Disposition of paeoniflorin and paeonimetabolin I in rats after oral administration of Toki-Shakuyaku-San (TS) and Shakuyaku-Kanzo-To (SK),
Meselhy, Meselhy R., Heikal Ola A., Akao Teruaki, KHOJIMATOV OLIM, Hattori Masao, ONO HIROMASA, and SADAKANE CHIHARU
, Natural medicines= 生薬學雜誌, Volume 52, Issue 3, p.265-268, (1998)
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Effect of tinctormine on contraction and Ca2+ currents in single cardiac myocytes from dog,
Meselhy, M. R., Momose Y., Hatakeyama N., Kadota S., Hattori M., and Namba T.
, Phytomedicine, Volume 1, Issue 4, p.277-281, (1995)
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Preparation of Lignan-Rich Extract from the Aerial Parts of Using Nonconventional Methods.,
Meselhy, Meselhy R., Abdel-Sattar Ola E., El-Mekkawy Sahar, El-Desoky Ahmed M., Mohamed Shanaz O., Mohsen Sobhy M., Abdel-Sattar Essam, and Halawany Ali El-
, Molecules (Basel, Switzerland), Volume 25, Issue 5, (2020)
AbstractPhyllanthin and related lignans were found to be responsible, at least in part, for most of the activity of species. This observation encouraged the authors to develop methods for the preparation of an extract rich in phyllanthin and related lignans from the aerial parts of L. Direct extraction with solvents produced extracts with variable yields and contents of lignans. Lignans were identified by LC-ESI-MS analysis as phyllanthin (used as marker substance), hypophyllanthin, phylltetralin, nirtetralin, and niranthin. Extraction with boiling water produced 18.10 g% (/) extract with a trace amount of lignans (phyllanthin content of 0.33 ± 0.10 mg/g extract), while extraction with MeOH gave 3.6 g% / extract with a low phyllanthin content (3.1 mg/g extract), as determined by HPLC. However, Soxhlet extraction with hexane, CHCl, or acetone gave extracts with low yields (0.82, 1.12, and 3.40 g% /, respectively) and a higher phyllanthin contents (36.2 ± 2.6, 11.7 ± 1.68, and 11.7 ± 1.10 mg/g extract, respectively). Extraction quality and efficiency were optimized by adopting the following three different approaches: (1) Alkaline digestion of the plant material with 30% potassium hydroxide yielded 3.1 g% / of purified extract with high phyllanthin content (22.34 ± 0.13 mg/g); (2) microwave-assisted extraction using 80% MeOH gave an extract with a better yield (8.13 g% /) and phyllanthin content (21.2 ± 1.30 mg/g) (after filtration through a Diaion HP-20 column); and (3) treatment of the ground plant material at 50 °C with two hydrolytic enzymes, cellulase (9 U/g for 12 h) and then, protease (4 U/g up to 72 h) optimized the yield of extract (13.92 g% /) and phyllanthin content (25.9 mg/g extract and total lignans content of 85.87 mg/g extract). In conclusion, the nonconventional methods presented here are superior for optimizing the yield of extract and its lignan contents from the aerial parts of .
Biotransformation of shikonin by human intestinal bacteria,
Meselhy, Meselhy R., KADOTA Shigetoshi, Tsubono Koji, Hattori Masao, and NAMBA Tsuneo
, Tetrahedron, Volume 50, Issue 10, p.3081-3098, (1994)
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Metabolism of safflor yellow B by human intestinal bacteria,
Meselhy, M. R., KADOTA Shigetoshi, Hattori Masao, and NAMBA Tsuneo
, Journal of natural products, Volume 56, Issue 1, p.39-45, (1993)
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TINCTRMINE, A NOVEL Ca2+ ANTAGONIST N-CONTAINING QUINOCHALCONE C-GLYCOSIDE FROM CARTHAMUS TINCTORIUS L.,
Meselhy, Meselhy R., KADOTA Shigetoshi, Momose Yasunori, Hattori Masao, and NAMBA Tsuneo
, Chemical and pharmaceutical bulletin, Volume 40, Issue 12, p.3355-3357, (1992)
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