@article {204853,
	title = {UPLC-PDA-ESI/MS metabolic profiling of dill shoots bioactive fraction; evidence of its antioxidant and hepatoprotective effects in vitro and in vivo.},
	journal = {Journal of food biochemistry},
	year = {2021},
	pages = {e13741},
	abstract = {<p>Hydroxyl radical ( OH) scavenging capacity of aqueous dill (Anethum graveolens L.) shoot (ADSh) extract was assessed using electron paramagnetic resonance (EPR) spectroscopy. ADSh extract (at concentrations of 0.5 and 10~mg/ml) exerted high (OH) radical scavenging power. ADSh extract was further fractionated on Diaion HP-20 column to yield five fractions. EPR spin-trapping assay revealed fraction 4 (eluted with 75\% aq. MeOH) to possess ( OH) radical scavenging capacity over a concentration range (0.01-10~mg/ml), whereas fraction 2 (eluted with 25\% aq. MeOH) appeared to be pro-oxidant at concentration 0.01~mg/ml. UPLC-PDA-ESI-MS metabolite profiling of ADSh extract revealed 87 metabolites, of which 64 compounds were identified in fraction 4, the most active fraction. Furthermore, ADSh extract demonstrated a hepatoprotective effect against acetaminophen (APAP)-induced hepatotoxicity in rats. Pretreatment of rats with ADSh extract (200~mg/kg b.wt) markedly attenuated the increased in the serum hepatic enzyme levels. It also increased free glutathione level and total antioxidant capacity in the serum of treated rats. [Correction added on May 3, 2021, after first online publication: "rates" has been changed to "rats" in the previous sentence.] Additionally, levels of (TNF-α and IL-1β) were back to almost normal levels compared to the control group. The above findings suggest that ADSh extract has a protective effect against APAP-induced liver damage.</p>
},
	author = {Rasheed, Dalia M and Ayat M. Emad and Sherifa F. Ali and Ali, Sameh S and Mohamed A. Farag and Meselhy, Meselhy R and Essam A. Sattar}
}
