Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets

Citation:
Elkady, E. F., and M. A. Mahrouse, "Validated Spectrophotometric Methods for the Simultaneous Determination of Ciprofloxacin Hydrochloride and Metronidazole in Tablets", Chem. Pharm. Bull., 2011.

Abstract:

A binary mixture of ciprofloxacin hydrochloride (CIP) and metronidazole (MET) was determined by five simple and accurate methods, without prior separation. In the first method, CIP was determined by second derivative spectrophotometric method (2D) by measuring the amplitude at 282 nm (zero ordinate value of MET). On the other hand, the determination of MET was based on isosbestic point technique, where the total content of the mixture was determined at 294.5 nm (isosbestic point), then the content of MET could be calculated by subtraction. The second method was first derivative ratio spectrophotometric method (1DD) where the total amplitude at 261 and 285 nm and the amplitude at 295.5 nm were selected to simultaneously determine CIP and MET in binary mixture, respectively. The third method was based on dual wavelength analysis, in which two wavelengths were selected, at which the absorbances of the other component were the same.

Notes:

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