A Study of Interleukin-10 (IL-10) Promoter Gene Polymorphisms and Response to Therapy in Chronic Hepatitis C Infection in Egyptian Children

Citation:
Mohamed, N. H. A., S. A. E. - A. Sharaf, I. A. Mandour, and H. M. El-Karaksy, A Study of Interleukin-10 (IL-10) Promoter Gene Polymorphisms and Response to Therapy in Chronic Hepatitis C Infection in Egyptian Children, , Egypt, Cairo University, 2013.

Thesis Type:

M.Sc Thesis

Abstract:

Background: According to the world Health Organization (WHO) report
(2002), at least 170 million people are chronically infected with hepatitis C virus
(HCV).Egypt with the highest prevalence of HCV infection (15%), its rural
villages have a high prevalence of HCV infection in children younger than 10
years of age. Interleukin 10 (IL-10) an anti-inflammatory cytokine, down regulates
the protective inflammatory response, adversely affecting the response to antiviral
treatment. The IL-10 promoter is highly polymorphic, two single nucleotide
polymorphisms (SNPs) G1082A and C592A that form three haplotypes (AA, AC,
and GC) have been shown to be associated with differential IL-10 expression in
humans. Aim of work: determine the prevalence of the 2 SNPs G1082A and
C592A in the IL-10 promotor region and their effect on response to antiviral
therapy in a cohort of children and young adults with HCV infection.
Patients and methods: forty HCV patients underwent baseline quantitation of
HCV-RNA by polymerase chain reaction (PCR) and baseline biochemical testing
and were followed up for seventy-two weeks, both clinically and via laboratory
assessment HCV-RNA viral load and liver function tests. The genotype status of
IL-10 was assessed by real time PCR-Taqman probe based assay. Results and
conclusion: there was no significant association between polymorphisms in the
IL-10 gene (G1082A and C592A) or cytokine haplotype as regards response to
therapy or severity of HCV infection in children. As for the SNP C592A; there
was a statistically significant association between the score of fibrosis and
different genotypes (P < 0.004), concluding that the (A) allele is risky. HCV RNAcount
and gamma glutamyl transferase pretreatment levels were found to be
predictors of response to interferon therapy in HCV infected children in this study.

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