Solvent-Free Microwave Extraction and Hepatoprotective Activity of Cyperus esculentus L

Citation:
H.D.Hassanein, N. M. Nazif, E. A. Aboutabl, and F. M. Hammouda, "Solvent-Free Microwave Extraction and Hepatoprotective Activity of Cyperus esculentus L", Journal of Applied Sciences Research; 7(12): 2455-2461 (2011), 2011.

Abstract:

Solvent-free microwave extraction (SFME) has been applied to the extraction of essential oils from Cyperus esculentus and Cyperus articulatus tubers. The composition of the essential oils were identified by GC/MS. The essential oil obtained with SFME contained substantially higher amounts of oxygenated compounds and lower amounts of monoterpenes. Essential oils which were obtained from C.articulatus and C.esculentus were characterized by much larger amounts of sesquiterpenes (67.4% and 64.6%, repectively) than monoterpenes (0.81% and 20.68%, respectively). C.articulatus essential oil was also characterized by higher content of nonterpenoid compounds than C.esculentus (25.53% and 12.09%) for tubers, respectively. Culture of monolayer of rat hepatocytes was applied as an in vitro method for investigation of the hepatoprotective and hepatotoxic effects of essential oils. C.esculentus and C.articulatus tubers essential oils showed no hepatotoxic effect till a concentration of 1000 ?g/mL. The hepatoprotective effects of C.esculentus and C.articulatus tubers essential oils, against the toxic effect of 25 mM paracetamol are 18.5 and 40 ?g/mL, respectively.

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