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Shalaby, R. S., M. M. Gaafar, M. E. A. Basshar, M. S. Soliman, M. M. Yousry, and Y. A. ElMahdy, "Clinical Study of Coexistence of Fungal Infections in Diabetic Foot Ulcers by 18s rRNA Gene Polymerase Chain Reaction", Open Access Macedonian Journal of Medical Sciences, vol. 11, issue A, 2023.
Soliman, M. S., S. M. Khairat, N. M. Reda, and N. S. Soliman, "Evaluating The Performance Of Two Rapid Antigen Detection Tests in Diagnosis of SARS-COV-2 Infection", Microbes and Infectious Diseases, 2022.
Moubasher, H., A. Elkholy, M. Sherif, M. Zahran, and S. Elnagdy, "In Vitro Investigation of the Impact of Bacterial–Fungal Interaction on Carbapenem-Resistant Klebsiella pneumoniae", Molecules , vol. 27, issue 8, pp. 2541, 2022.
D, J., T. H.S, H. W, B. U, E. N. M., A. K, S. O, Y. A, D. A.A, E. - S. H.E, et al., "SARS-CoV-2 genome variations and evolution patterns in Egypt: a multi-center study", Scientific Reports, vol. 12, issue 1, pp. 14511, 2022.
Amr Abulhamd Sayed Othma, H. E. E. Gomaa, M. G. ElAnany, E. M. A. Rahman, E. M. Hassan, A. A. M. Elbaky, M. M. S. Soliman, and E. Awadallah, "Use of multiplex PCR in diagnosis of childhood acute viral diarrhoea caused by rotavirus, norovirus, astrovirus and adenovirus in Upper Egypt", Egyptian Journal of Medical Human Genetics, vol. 23, issue 1, pp. 1-9, 2022.
Zekri, A. - R. N., A. A. Bahnasy, M. M. Hafez, Z. K. Hassan, O. S. Ahmed, H. K. Soliman, E. R. El-Sisi, M. H. Dine, M. S. Solimane, L. S, et al., "Characterization of the SARS-CoV-2 genomes in Egypt in first and second waves of infection", Scientific Reports, vol. 11, issue 1, pp. 1-11, 2021.
Soliman, M. S., M. Abdelfattah, S. M. N. Aman, L. M. Ibrahim, and R. K. Aziz, "A Gapless, Unambiguous Rna Metagenome-Assembled Genome Sequence of a Unique SARS-CoV-2 Variant Encoding Spike S813i and Orf1a A859v Substitutions", OMICS: A Journal of Integrative Biology, vol. 25, issue 2, pp. 123-128, 2021.
Zekri, A. - R. N., K. Easa Amer, M. M. Hafez, Z. K. Hassan, O. S. Ahmed, H. K. Soliman, A. A. Bahnasy, W. Abdel Hamid, A. Gad, M. Ali, et al., "Genomic characterization of SARS-CoV-2 in Egypt", Journal of advanced research, vol. 30, pp. 123-132, 2021.
Essam, S., N. N. Nawar, M. ElBashaar, M. Soliman, and M. Abdelfattah, "In Vitro Activity of Plazomicin among Carbapenem-resistant Enterobacteriaceae", Open Access Macedonian Journal of Medical Sciences., vol. 9, 2021.
O., S., R. A.A, E. H., S. A., A. - R. T.M, E. A., S. M.S., B. E.B., and S. K, "Urethral instillation of chlorhexidine gel is an effective method of sterilisation", Arab Journal of Urology, vol. 19, issue 3, pp. 419-422, 2021.
Sherif, M., M. Palmieri, C. Mirande, H. El-Mahallawy, H. G. Rashed, F. Abd-El-Reheem, A. R. El-Manakhly, R. A. R. Abdel-Latif, A. G. Aboulela, L. Y. Saeed, et al., "Whole-genome sequencing of Egyptian multidrug-resistant Klebsiella pneumoniae isolates: a multi-center pilot study.", European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2021. Abstract

Multidrug-resistant (MDR) Klebsiella pneumoniae is a common infectious pathogen. We performed whole-genome sequencing (WGS) of 39 randomly selected, geographically diverse MDR K. pneumoniae from nine Egyptian hospitals. Clinical sources, phenotypic antibiotic resistance, and hyper-mucoviscosity were documented. WGS data were epidemiologically interpreted and tested for the presence of antibiotic resistance and virulence genes. Based on WGS data, we identified 18 classical multi-locus sequence types (MLST), the most common type being ST101 (23.1%) followed by ST147 (17.9%). Phylogenetic analyses identified small numbers of closely related isolates in a few of the centers, so we mostly documented independent nosocomial acquisition or import from public sources. The most common acquired resistance gene found was bla, detected in 27 isolates (69.2%). Carbapenemase genes encountered were bla (n = 13), bla (n = 1), bla (n = 12), bla (n = 2), and bla (n = 1). Seven strains (18%) contained more than a single carbapenemase gene. While searching for virulence-associated genes, sixteen wzi alleles were identified with wzi137, wzi64, and wzi50 most commonly found in ST101, ST147, and ST16, respectively. Yersiniabactin was the most common virulence factor (69.2%). Hyper-mucoviscosity was documented for 6 out of 39 isolates.This is the first genomic study of MDR K. pneumoniae from Egypt. The study revealed a clear spread of well-known international clones and their associated antimicrobial resistance and (hyper)virulence traits. The clinical situation in Egypt seems to reflect the scenario documented in many other countries and requires close attention.

Soliman, M. S., M. M. Kamel, J. A. Alorabi, N. M. Mohamed, A. A. El-Kholy, and A. S. Abdel-Moneim, "Characterization and mutational analysis of haemagglutinin and neuraminidase of H3N2 and H1N1pdm09 human influenza A viruses in Egypt", VirusDis, vol. 31, issue 3, pp. 262–269, 2020.
Soliman, M. S., R. K. Aziz, N. S. Soliman, A. R. El-Manakhly, S. A. S. ElBanna, and A. A. El-Kholy, "Genomic Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) by High-Throughput Sequencing in a Tertiary Care Hospital", Genes, vol. 11, issue 2020:11, pp. 1219, 2020.
, "Genomic characterization of SARS-CoV-2 in Egypt", Journal of Advanced Research, 2020.
NS, F., G. D, Mohamed SH, W. RM, S. MS, E. - K. AA, and H. PG, "Molecular Characterization of Carbapenem/Colistin-Resistant Acinetobacter baumannii Clinical Isolates from Egypt by Whole-Genome Sequencing", Infection and Drug Resistance, vol. 2020, issue 13, pp. 4487—4493, 2020.
Fam, N. S., S. Mohamed, D. Gamal, R. M. Wasfy, M. S. Soliman, and A. A. El-Kholy, "Reliability of phenotypic methods for detection of colistin resistance among carbapenem-resistant clinical isolates from Egypt.", Germs, vol. 10, issue 4, pp. 303-309, 2020. Abstract

Introduction: is a challenging pathogen responsible for serious nosocomial infections. Colistin resistance in carbapenem-resistant strains is a critical health problem as it limits the available therapeutic options. The current work aimed to study the reliability of several phenotypic methods for the detection of colistin resistance among carbapenem-resistant isolates in Egypt.

Methods: A total of 22 carbapenem-resistant isolates were recovered. Colistin minimum inhibitory concentrations (MICs) were determined using broth microdilution (BMD) and compared to agar dilution (AD), automated system (VITEK-2) and gradient test (E-test) and were analyzed by statistical methods.

Results: Phenotypic testing showed that nine of 22 isolates (40.9%) were colistin-resistant by BMD and seven of them were also resistant by AD, with the categorical agreement (CA) of 72.7% and essential agreement (EA) of 90.9%. Colistin MIC results ranged from 1-8 µg/mL and 1-32 µg/mL by both AD and BMD respectively. Detection of colistin resistance by gradient test and automated system showed high very major error (VME) rates (40.9%) compared to BMD with a lack of CA between them. AD gave moderate agreement with BMD by 90.9% EA, 72.7% CA and only 9.1% VME.

Conclusions: In delineating colistin breakpoints BMD followed by AD method are defined as the only reliable phenotypic methods for colistin resistance evaluation. More rapid and reliable tests, other than BMD and AD, are required for the convenient detection of colistin resistance in the routine clinical microbiology laboratory daily workflow.

el Kholy, A., M. Badawy, M. Gad, and M. Soliman, "Serotypes and Antimicrobial Susceptibility of Nasopharyngeal Isolates of Streptococcus pneumoniae from Children Less Than 5 Years Old in Egypt", Infection and Drug Resistance, issue 2020:13, pp. 3669–3677, 2020.
E.abuelhassan, U., M. S. Rezk, M. M. S. Soliman, M. Al-Harrass, T. Saber, and T. Saber, "Role of Antiviral Therapies in Laboratory Confirmed Influenza Cases in Egypt 2010-2012/Single Center Experience Study", EC Pulmonology and Respiratory Medicine, vol. 8.8 , pp. 695-700 , 2019.
El-Kholy, A. A., M. G. El Anany, M. M. Sherif, and M. A. Gad, "High Prevalence of VIM, KPC, and NDM Expression among Surgical Site Infection Pathogens in Patients Having Emergency Surgery", Surgical Infections, vol. 19, issue 6, 2018.
Baroudy, N. E. R., A. E. S. Refay, T. A. A. Hamid, D. M. Hassan, and M. S. Soliman, "Respiratory Viruses and Atypical Bacteria Co-Infection in Children with Acute Respiratory Infection", Open Access Macedonian Journal of Medical Sciences., 2018.
Abdel-Moneim, A. S., M. S. Soliman, M. M. Kamel, and A. A. El-Kholy, Sequence analysis of the G gene of hRSVA ON1 genotype from Egyptian children with acute respiratory tract infections, , 2018.
Al-Rachidi, N. G., A. A. El-kholy, M. G. El Anany, M. S. Soliman, R. M. Hassan, R. M. Hassan, D. M. Bassiouny, and H. H. Rizk, "Early diagnosis of infective endocarditis using a commercial multiplex PCR assay in Egypt", Kasr Al Ainy Medical Journal, vol. 23, issue 2, pp. 62-72, 2017. kasralainymedj23267-1092978_030209.pdf