, "The Impact of Cinnamon Oil on Hepatorenal Toxicity and Antioxidant Related Gene Expression Induced by Deltamethrin in Ra", Advances in Animal and Veterinary Sciences, 2021, 9(7), pp. 1071–1077, vol. 9, issue 7, pp. 1071-1, 2021.
Ahmed W.M.S., Abdel-Azeem N.M., I. M.A., and R. A. M. Helmy N.A., "Neuromodulatory effect of cinnamon oil on behavioural disturbance, CYP1A1, iNOStranscripts and neurochemical alterations induced by deltamethrin in rat brain", ecotoxicology and environmental safety, vol. -, issue -, pp. -, 2021.
Ahmed W.M.S., Abdel-Azeem N.M., I. M.A., and R. A. M. Helmy N.A., "Neuromodulatory effect of cinnamon oil on behavioural disturbance, CYP1A1, iNOStranscripts and neurochemical alterations induced by deltamethrin in rat brain", ecotoxicology and environmental safety, vol. -, issue -, pp. -, 2021.
Ahmed W.M.S., Abdel-Azeem N.M., I. M.A., and R. A. M. Helmy N.A., "Neuromodulatory effect of cinnamon oil on behavioural disturbance, CYP1A1, iNOStranscripts and neurochemical alterations induced by deltamethrin in rat brain", ecotoxicology and environmental safety, vol. -, issue -, pp. -, 2021.
Marwa A Ibrahim, A. M. El Behairy, M. A. Ghoneim, and H. A. Amer, "Protective effect of curcumin and chlorophyllin against DNA mutation induced by cyclophosphamide or benzo[a]pyrene.", Zeitschrift fur Naturforschung. C, Journal of biosciences, vol. 62, issue 3-4, pp. 215-22, 2007 Mar-Apr. Abstract

The current study was carried out to evaluate the potency of curcumin and chlorophyllin as natural antioxidants to reduce the oxidative stress markers induced by cyclophosphamide (CP) and benzo[a]pyrene [B(a)P] which were used as free radical inducers. For this purpose, 126 male albino rats were used. The animals were assigned into 4 main groups: negative control group; oxidant-treated group (subdivided into two subgroups: cyclophosphamide-treated group and benzo[a]pyrene-treated group); curcumin-treated group; and chlorophyllin-treated group. Liver samples were collected after two days post the oxidant inoculation and at the end of the experimental period (10 weeks). These samples were examined for determination of liver microsomal malondialdehyde (MDA), DNA fragmentation, restriction fragment length polymorphism (RFLP) and 8-hydroxy deoxyguanosine (8-OHdG) concentration. Both CP and B(a)P caused increments in DNA fragmentation percentages, liver microsomal MDA, concentration of 8-OHdG and induced point mutation. Treatment of rats with either curcumin or chlorophyllin revealed lower DNA fragmentation percentages, liver microsomal MDA concentration, concentration of 8-OHdG and prevented induction of mutations, i.e., reversed the oxidative stress induced by CP and B(a)P and proved that they were capable of protecting rats against the oxidative damage evoked by these oxidants.

Ibrahem, M. D., H. M. Khairy, and Marwa A Ibrahim, "Laboratory exposure of Oreochromis niloticus to crude microcystins (containing microcystin-LR) extracted from Egyptian locally isolated strain (Microcystis aeruginosa Kützing): biological and biochemical studies.", Fish physiology and biochemistry, vol. 38, issue 3, pp. 899-908, 2012 Jun. Abstract

Cyanobacterial blooms exert negative impacts on fisheries and water management authorities. Recently, it has gained global attention, as elevated earth warming and environmental pollution are accelerating algal growth. Oreochromis niloticus (O. niloticus) is a worldwide and the most commonly cultured fish in Egypt. The biological interaction of the living organisms to the surrounding environment must continuously be assessed to predict future effects of the ongoing hazards on fish. The study was designed to examine the possible biological and biochemical response of O. niloticus exposed to different concentrations of microcystins crude extract (containing microcystin-LR). Three equal groups of O. niloticus were assigned for intraperitoneal injection of three different doses: 100, 200, and 400 μg m(-1) dried aqueous microcystins extract, for 10 days. Clinical, condition factor (K) and hepatosomatic index (HIS) were estimated. Biochemical alterations were evaluated via lipid peroxidation, DNA fragmentation assay and electrophoretic analysis of fragmented DNA using agarose gel electrophoresis. The results showed that there were discernible behavioral and clinical alterations. Significant differences in K and HIS were observed between treatments. Also, significant elevations were observed in lipid peroxidation level and in the DNA fragmentation percentage in the exposed fish to the doses of 200 and 400 μg m(-1) of microcystins crude extract. The current study addresses the possible toxic effects of microcystins crude extract to O. niloticus. The results cleared that microcystins crude extract (containing MC-LR) is toxic to O. niloticus in time- and dose-dependent manners.

Khairy, H. M., Marwa A Ibrahim, and M. D. Ibrahem, "Phenological and liver antioxidant profiles of adult Nile tilapia (Oreochromis niloticus) exposed to toxic live cyanobacterium (Microcystis aeruginosa Kützing) cells.", Zeitschrift fur Naturforschung. C, Journal of biosciences, vol. 67, issue 11-12, pp. 620-8, 2012 Nov-Dec. Abstract

Blue-green algae (cyanobacteria) constitute the greater part of the phytoplankton. Microcystis aeruginosa is amongst the most ubiquitously distributed cyanobacterial species, and almost invariably produces cyclic heptapeptide toxins called microcystins (MCs). The present study was designed to investigate the phenological and liver antioxidant profiles of the Nile tilapia Oreochromis niloticus chronically exposed to toxic live M. aeruginosa cells. Fish were grown in the absence and presence of M. aeruginosa in three different concentrations for seven days, and subsequently reared for another 30 days in the absence of the cyanobacteria. While cyanobacteria did not cause any fish mortality, there was a progressive development of yellowish discolouration in the livers of exposed fish. In the livers, the activities and levels of superoxide dismutase (SOD), lactate dehydrogenase (LDH), glutathione (GSH), and lipid peroxidation products like malondialdehyde (MDA) were elevated in response to the concentration of M. aeruginosa. Moreover, DNA fragmentation and DNA-protein crosslinks were measured. These parameters can thus be considered potential biomarkers for the fish exposure to M. aeruginosa. The present study sheds light on cyanobacterial blooms like health, environmental, and economic problem, respectively.

Ibrahem, M. D., and Marwa A Ibrahim, "The potential effects of Spirulina platensis (Arthrospira platensis) on tissue protection of Nile tilapia (Oreochromis niloticus) through estimation of P53 level.", Journal of advanced research, vol. 5, issue 1, pp. 133-6, 2014 Jan. Abstract

The current study was designed to investigate the potential effect of Spirulina platensis, Arthrospira platensis, (SP) on tissue protection of Nile tilapia (Oreochromis niloticus) through estimation of P53 level. Five isonitrogenous and isocaloric rations containing graded levels of dried SP 5, 7.5,10, 15, and 20 g/kg diet were fed separately to five equal groups of O. niloticus fingerlings, additional control group was assigned for 3 months. Liver samples were separately collected from each group by the end of each month. The expression level of P53 showed a substantial decrease among the treated groups in a time-dependent manner. It is therefore advisable to incorporate SP in diets for tissue protection and antioxidant effects in cultured O. niloticus.

Mona K Galal, Abdel Azim A Khalaf, H. A. Ogaly, and Marwa A Ibrahim, "Vitamin E attenuates neurotoxicity induced by deltamethrin in rats.", BMC complementary and alternative medicine, vol. 14, pp. 458, 2014 Dec 02. Abstract

BACKGROUND: The safety of Deltamethrin (DM) has been raised as a point of concern. The current investigation was envisaged to explore the responsiveness of oxidative stress parameters, DNA fragmentation and expression levels of TP53, cycloxygenase 2 (COX2) and cytochrome p4502E1 (CYP2E1) as toxicological endpoint in rats treated with DM. as well as attention was provided to the neuroprotective effect of vitamin E (VE).

METHODS: Four different groups of rats were used in this study, group I served as control, group II received DM (0.6 mg/kg BW), group III received both DM plus VE and finally group IV received VE only (200 mg/kg BW). The treatment regimen was extending for one month for all groups and the brain tissues were collected for further analysis.

RESULTS: The obtained results showed a highly statistically significant increase in lipid peroxidation (LPO) content, nitric oxide concentration, and DNA fragmentation percentage and expression level of CYP2E1, TP53 and COX2 genes, in addition statistical significant reduction in total antioxidant capacity in DM treated group as compared to control were detected. Oral administration of VE attenuated the neurotoxic effects of DM through improvement of oxidative status, DNA fragmentation percentage and suppressing the expression level of CYP2E1, TP53 and COX2 genes.

CONCLUSION: From this study we concluded that VE supplementation has beneficial impacts on DM neurotoxicity in rats through its antioxidant and antiapoptotic properties.

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