Marwa Mahmoud Bedier

Mineral trioxide aggregate (MTA), has been introduced in endodontics for its advantageous properties of biocompatibility; especially its ability to enhance cementogenesis, promoting healing and good sealing ability.

   Although, MTA has many advantageous properties, yet, the are some disadvantages, such as:

To compare between White Mineral trioxide aggregate (MTA) and White Portland cement (PC) regarding their:

Antibacterial activity using the    “Direct Contact Test”.

Sealing ability as root-end filling materials in differently-prepared root-end cavities using the “Dye Extraction Method”.

Part I: Antibacterial activity

1- Test microorganisms and growth

    conditions:

       The two materials were evaluated against the following facultative bacterial species: Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus.

2- Evaluation of the antibacterial activity:

a- Preparation of the samples :

b- Spectrophotometric measurments :

Part II: Sealing ability

1- Selection and preparation

    of the  samples:

2- Preparation and obturation

   of the root canals:

3- Root-end resection and

    root-end preparation:

6. Microleakage assessment using the dye  
   extraction method:

Part II: Sealing ability

The vials were centrifuged at 4000 rpm for 15 min.

One hundred microliter of the supernatant were taken and transferred to a well in a 96-well plate.

The optical density of the solutions in the 96-well plate was determined using spectrophotometer.

Part I: Antibacterial activity

       A. Enterococcus  faecalis

B. Pseudomonas aeruginosa

B. Pseudomonas aeruginosa

Part II: Sealing ability