Rashid, E. M., F. S. A. El-Hashemy, Z. A. Thabet, and M. R. Ali, "Tracing the efficiency of food processing steps on the pesticide residuals reduction rate in fruits and vegetables", African Journal of Biological Sciences, vol. 6, issue 4, pp. 165-178, 2024. african8.pdf
Ali, M. R., H. H. Ibrahim, and A. A. Salah-Eldin, "Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew's Mallow Stems as a Promising Anticancer Agent.", Molecules (Basel, Switzerland), vol. 29, issue 6, 2024. Abstractmolecules-29-01377.pdf

Phytochemicals from waste materials generated by agricultural and industrial processes have become globally significant due to their accessibility and potential effectiveness with few side effects. These compounds have essential implications in both medicine and the economy. Therefore, a quantitative analysis of the phytochemical profile, sugar types, and water-soluble vitamins of dried L."DJMS" extract (dried Jew's mallow stem) was carried out with HPLC. In addition, the chemical composition, TPC, chlorophyll a and b, beta-carotene, and antioxidant effect using DPPH were investigated. Furthermore, the anticancer activity of the DJMS was evaluated by SRB assay using Huh-7 and MDA-MB-231 cell lines. In the quantitative study, DJMS extract showed a high antioxidant potential (67%) due to its content of bioactive compounds such as TPC (276.37 mg 100 g) and chlorophyll a and b (20.31, 12.02 mg 100 g, respectively), as well as some vitamins and minerals such as B-complex (B12; 146.8 mg 100 g and vitamin C 6.49 mg 100 g) and selenium (<0.2 μg kg). Moreover, the main sugar types found were sucrose and stachyose, which recorded 9.23 and 6.25 mg 100 g, respectively. Identifying phenolic and flavonoids showed that the major components were ellagic acid (4905.26 μg kg), ferulic acid (3628.29 μg kg), chlorogenic acid (3757.08 μg kg), luteolin-7--glucoside (4314.48 μg kg), naringin (4296.94 μg kg) and apigenin-6-rhamnose-8 glucoside (3078.87 μg kg). The dried stem extract showed significant MDA-MB-231 inhibition activity and reached 80% at a concentration of 1000 µg/mL of DJMS extract, related to the content of phytochemical components such as isoflavones like genistein (34.96 μg kg), which had a tremendous anticancer effect. Hence, the stem of Jew's mallow (which is edible and characterized by its viability and low production cost) possesses the capacity to serve as a pharmaceutical agent for combating cancer owing to its abundance of bioactive components.

Lakshmanan, G., A. B. Altemimi, C. Sivaraj, J. Selvakumari, L. Karthik, K. Saravanan, V. Viswanathan, A. Pandian, F. Cacciola, M. R. Ali, et al., "Imperatorin from the aerial parts of Cleome viscosa L.: a characterization study and evaluation of the antibacterial activity", Natural Product Research, vol. 38, issue 5, pp. 848-855, 2024.
El-Beltagi, H. S., M. R. Ali, K. M. A. RAMADAN, R. Anwar, T. A. Shalaby, A. A. Rezk, S. M. El-Ganainy, S. F. Mahmoud, M. Alkafafy, and M. M. El-Mogy, "Exogenous Postharvest Application of Calcium Chloride and Salicylic Acid to Maintain the Quality of Broccoli Florets.", Plants (Basel, Switzerland), vol. 11, issue 11, 2022. Abstract

The importance of broccoli ( var. ) consumption has increased in recent years due to its significant amount of anticarcinogenic and antioxidant compounds, as well as its many vitamins. However, broccoli florets are a highly perishable product which rapidly senesce and turn yellow after harvest, resulting in losses in nutritional and bioactive compounds. Thus, in this study, we evaluated the effect of postharvest exogenous of salicylic acid (SA) and calcium chloride (CaCl) and their combination on the quality of broccoli florets stored at 5 °C for 28 days to minimize the rapid senescence of broccoli florets. Samples treated with 2 mM SA alone or in combination with 2% CaCl showed lower weight loss and lower losses of chlorophyll content, vitamin C, phenolic compounds, carotenoids, flavonoids, and glucosinolates compared with the control samples. Additionally, antioxidant activity was maintained by either SA or SA + CaCl treatments while peroxidase activity was decreased. For higher quality and lower losses in antioxidant compounds of broccoli florets during refrigerated storage at 5 °C, SA + CaCl treatment could be helpful for up to 21 days.

Abuhlega, T. A., and M. R. Ali, "Biogenic amines in fish: Prevention and reduction", Journal of Food Processing and Preservation , vol. 46, issue e16883, pp. 1-15, 2022. food_processing_preservation_-_2022_-_abuhlega_-_biogenic_amines_in_fish__prevention_and_reduction.pdf
El-Beltagi, H. S., M. M. El-Mogy, A. Parmar, A. T. Mansour, T. A. Shalaby, and M. R. Ali, "Phytochemical Characterization and Utilization of Dried Red Beetroot () Peel Extract in Maintaining the Quality of Nile Fish Fillet.", Antioxidants (Basel, Switzerland), vol. 11, issue 5, 2022. Abstractantioxidants-11-00906.pdf

Phytochemicals derived from agro-industrial waste materials could be employed as functional food additives and natural antioxidants to replace their synthetic counterparts, which are increasingly being rejected. The current study aims to assess total phenolic compound (TPC), flavonoids, betalain contents, and antiradical scavenging using DPPH and IC50% of dried red beetroot peel (DRBP) extract at different concentrations of 50, 80, 100, 150, and 200 mg/100 mL t. In addition, a characterization of phenols and flavonoids was conducted using HPLC. The second part of this study aims to utilize aqueous DRBP extract in preserving Nile fish fillet at two concentrations of 80 and 100 mg/100 mL water, compared with 200 ppm of BHT (butylated hydroxytoluene) and control at 5 °C for 10 days. The DRBP aqueous extract was found to have a high concentration of TPC (832 mg/100 g), flavonoids (234 mg/100 g) and betalains (535 mg/100 g) compounds, resulting in a potential antioxidant activity. The IC50% for the extract was detected at 80 mg/100 mL extract. DRBP aqueous extract showed an excellent preservative effect on the fish fillet. Fish fillet samples treated with DRBP extract at a concentration of 100 mg/100 mL were superior in reducing TBA (thiobarbituric acid) increase compared with other treatments at the end of cold storage. Overall, the study showed that red beetroot extracts can act as a natural preservative agent due to their significant antioxidant activity, providing healthy and safe food to consumers.

Abdelgawad, K. F., A. H. R. Awad, M. R. Ali, R. A. Ludlow, T. Chen, and M. M. El-Mogy, "Increasing the Storability of Fresh-Cut Green Beans by Using Chitosan as a Carrier for Tea Tree and Peppermint Essential Oils and Ascorbic Acid.", Plants (Basel, Switzerland), vol. 11, issue 6, 2022. Abstractplants-11-00783.pdf

The quality of fresh-cut green beans deteriorates rapidly in storage, which contributes to increased food waste and lower perceived customer value. However, chitosan (Cs) and certain plant essential oils show promise in reducing postharvest quality loss during storage. Here, the effect of Cs and the combinations of Cs + tea tree oil (TTO), Cs +x peppermint oil (PMO), and Cs + ascorbic acid (AsA) on the quality of fresh-cut green bean pods (FC-GB) is studied over a 15-d storage period at 5 °C. All four FC-GB treatments reduced weight loss and maintained firmness during storage when compared to uncoated FC-GB. Furthermore, all treatments showed higher total chlorophyll content, AsA, total phenolic compounds, and total sugars compared to the control. The best treatment for reducing microbial growth was a combination of Cs + AsA. Additionally, the combination of Cs with TTO, PMO, or AsA showed a significant reduction in the browning index and increased the antioxidant capacity of FC-GB up to 15 d postharvest.

Darwish, O. S., M. R. Ali, E. Khojah, B. N. Samra, K. M. A. RAMADAN, and M. M. El-Mogy, "Pre-Harvest Application of Salicylic Acid, Abscisic Acid, and Methyl Jasmonate Conserve Bioactive Compounds of Strawberry Fruits during Refrigerated Storage", Horticulturae, vol. 7, issue 12, pp. 568., 2021. horticulturae-07-00568.pdf
Mostafa, H. S., M. R. Ali, and R. M. Mohamed, "Production of a novel probiotic date juice with anti-proliferative activity against Hep-2 cancer cells", Food Science and Technology, issue 100, pp. 1-11, 2021. date_juice.pdf
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