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2007
abd elaziz, M., M. Ali, S. yassin, and A. S. raafat and rashad, "Study on the effects of some probiotics on the performance, clinicopathological and histopathological changes of Oreochromis niloticus", Veterinary Medical Journal, vol. 55, no.3 , pp. 879-897, 2007. Abstract

The presen study was carried out to evaluate the use of some probiotics on the growth rate of tilapia fishes and to determine its effect on some hematological and histopathological parameters. Two different commercial probiotics which includes biogen and moreyeast were evaluated for 8weeks. In the experiment, fifty Oreochromis niloticus fishes were classified to 3 groups,Group (1): consisted of 20 fishes which fed diet supplemented by biogen at a dose of 2 kg/ton diet. Group (2): consisted of 20 fishes which fed diet supplemented by moreyeast at a dose of 5 kg/ton diet and Group (3): was control group which consisted of 10 fishes fed diet without probiotics. At the end of the experimental period both biogen and moreyeast administrated groups revealed a significant increase in the body weight gain (W.G.). The results showed significant increase in the values of RBCs, PCV and Hb throughout the experimental period except group 1 (received biogen) at the 6th week of experiment comparing to control group. Fish group received moreyeast (Group 2) had the pronounced effect than group 1. Assessment of leukogram revealed significant leukocytosis allover the experimental period in different groups except during the 2nd week in group 2 (received moreyeast) and at the 6th week in both probiotic administrated groups comparing to control group. Fish group received biogen had pronounced effect comparing to moreyeast administrated group. differential leukocytic picture was recorded. Histopathological examination revealed The biogen and moreyeast administerd groups showed marked activation of the melano-macrophage centers which is a component of reticuloendothelial system and hence part of the defensive system of fish indicating immunoenhancment. the study concluded that: The examined probiotic were safe for the Oreochromis niloticus fish.

Khalil, W. - K. B., M. A. Mahmoud, M. M. Zahran, and K. F. Mahrous, "A sub-acute study of metronidazole toxicity assessed in Egyptian Tilapia zillii.", Journal of applied toxicology , vol. 27, pp. 380- 390, 2007. Abstract

Metronidazole (MTZ), an antiparasitic and antibacterial compound, is one of the world’s most widely used drugs. Despite being considered as a rodent mutagen and a carcinogen, it is still widely used in humans for the treatment of infections with anaerobic organisms. Therefore, the main objective of the current study was to evaluate the in vivo toxicity of MTZ using the micronucleus (MN) assay and random amplified polymorphism DNA (RAPD-PCR) analysis as well as histopathological examination in Tilapia zillii. Moreover, the protective effect of vitamin C (VitC) against toxicity of MTZ was investigated in the present study. Fish were treated with three doses of MTZ (5, 10 and 20 mg/ l) alone or in combination with VitC (200 mg/ kg food) at several time intervals (2 days, 7 days and 14 days). The results of the present study showed a significant effect of MTZ on micronucleus formation and changes in polymorphic band patterns as well as induction of different histopathological alterations in Tilapia zillii. The effects of the drug were reduced when fish were exposed to a combination of MTZ and VitC.

2006
Karima, F. M., K. B. K. Wagdy, and A. M. Mahmoud, "Assessment of toxicity and clastogenicity of sterigmatocystin in Egyptian Nile tilapia", African Journal of Biotechnology , vol. 5 (12), pp. 1180-1189., 2006. Abstract

The increasing presence of genotoxic pollutants in the aquatic environment has led to the development of quick monitoring methods. Sterigmatocystin (Stg) is closely related to mycotoxins and has the carcinogenic potency in the experimental animal models. The exposure to genotoxic agents will give rise to alterations of DNA structure that can lead to abnormal changes of DNA fingerprints. Therefore, we have applied the random amplified polymorphism DNA (RAPD) method to evaluate the genotoxic effects of Stg and to determine if the Egyptian montmorillonite (EM) has a protective effect against Stg. The experiment was conducted in vivo to evaluate the ability of EM at a level 0.5 mg/kg body weight (bw) to prevent the toxicity and genotoxicity induced by Stg in the Nile tilapia fish. Fishes were orally administrated with EM in corn oil with or without Stg (1.6 µg/kg bw) twice a week for 4 weeks. Blood and tissue samples were collected at the end of the treatment. The results revealed that Stg had genotoxic and toxicopathological effects in Oreochromis niloticus fish. The genotoxic effects were indicated by appearance of some changes in polymorphism band patterns including lost of stable bands or occurrence of new bands. There also exists a distinct distance between the band patterns of exposed fish and protected or control fish samples. The effects on the tissues were manifested by different histopathological lesions in different organs including hyperplastic proliferation of branchial epithelium, necrobiotic changes in hepatic tissue and destruction of components of the spleen. These responses were virtually abolished or markedly decreased when fishes were exposed to EM combined with Stg. It could be conclude that addition of EM resulted in the inhibition of the toxicity and clastogenicity of Stg.

Elsayed, E. E., N. E. E. Dien, and M. A. Mahmoud, "Ichthyophthiriasis: Various Fish Susceptibility or Presence of More than one Strain of the Parasite", Nature and Science, vol. 4 (3), pp. 5-13., 2006. Abstract

White spot disease is one of the devastating protozoal infections affecting freshwater fish. Commonly known as “Ich”, the Ichthyophthiriasis can infect almost all freshwater fish causing devastating losses in susceptible fish. In the present study, an outbreak of Ichthyophthiriasis erupted in one of the holding tanks of two ornamental fish species, Siamese shark (Pangasius sutchi) and goldfish (Carassius auratus var.bicausatus). Initial observation of the outbreak showed that only Pangasius sutchi was affected by typical white spots associated with mortalities. However, Carassius auratus, a known susceptible species for Ichthyophthirius multifiliis (Ich) in the same aquarium showed only mild erythema that disappeared during the course of infection with no mortalities. To confirm the previous observation, an experimental designs was performed in which infection with Ichthyophthirius was induced in Pangasius sutchi species alone. Cohabitation was performed between the Ich-induced Pangasius sutchi and Carassius auratus. Three days after the induction, Pangasius sutchi started showing the typical clinical signs. Mortalities associated with severe infection were recorded in Pangasius sutchi by 7th day after infection. Associated Carassius auratus showed only mild erythema that disappeared by the end of experiment. Histopathological examination of skin from both species in natural and experimental infection was performed to evaluate the severity of infection on the tissue level. Substantial numbers of typical large size trophonts surrounded by layers of fibrous tissue, melanophores and hemorrhages were detected in dermal and epidermal layers. Underlying myodegeneration was also associated the skin lesions in Pangasius sutchi. In contrary, pathological changes in the skin of Carassius auratus were mild and few numbers of immature trophonts were noticed in the epidermal layers. Possible reasons for such infection discrepancies between the two susceptible species are discussed.

MAI, I. D., H. A. Hussien, and A. M. Mahmoud, "Spring Viraemia of Carp disease: Experimental Infection of Cultured Common Carp (Cyprinus Carpio L.) in Egypt", Veterinary Medical Journal, vol. 54, no.4, pp. 887-898 , 2006. Abstract

Experimental oral infection of common carp (C. carpio L.) fish was carried out via stomach tube with 2 local positive samples of Spring Viraemia of Carp Virus (SVCV) at water temperature 14± 20C. Investigation of the pathogenesis of SVCV to common carp (C. carpio L.) revealed that virus was detected in gills at the 3rd day post infection, and in the internal organs one week post infection. Clinical and post mortem examination of experimentally infected fish were recorded. The established infection was confirmed using monoclonal antibodies against SVCV in dot ELISA technique and electron microscopy. Histopathological picture of the experimentally infected common carp (C. carpio L.) showed severe changes in gills, liver and intestine.

2005
Marzouk, M. S. M., M. M. Ali, B. SHALABY, A. M. Mahmoud, and M. I. ABD EL- SALAM, "A Contribution on anaerobic bacterial infection in cultured freshwater fish.", J. Egypt. Vet. Med. Ass., vol. 65(3), pp. 123-140., 2005. Abstract

In this work, the incidence and occurance of anaerobic bacterial pathogen in different freshwater fish farms have been investigated. The results indicated the presence of C. perfrengens, C. sporogenes, C. birfermentans, Actinomyces species and peptostreptococcus species in Nile tilapia ( O. niloticus), C. carpio in soil of earthen ponds using poultry droopings as organic fertilizers, while nile tilapia ( O. niloticus) collected from concrete ponds were negative for isolation of anaerobic bacteria.Typing of isolated C. perfringens indicated that the isolates were belonging to the toxogenic A and D types. Examination of water samples revealed the presence of great alterations in the physicochemical and microbiological parameters, particularly, in samples collected from earthen ponds.
The histopathological changes of experimentally inoculated fishes with toxogenic isolates of C. perfringens were fully demonstrated.

2004
Elsayed, E., M. A. Mahmoud, and N. E. ElDien, " Ichthiophthiriasis: Atypical outbreak In Two Suscetible Ornamental Fish Species In Egypt Under The Same Environmental Conditions ", 29th ANNUAL EASTERN FISH HEALTH WORKSHOP, U.S.A., March 22-26, 2004.
Sekena, H. A. E., M. A.Mahmoud, M. A.Abdel-Wahab, and M. F. Azza M. Hassan, Karima, " Chemoprevention of Barley and Sage against acrylamide-Induced genotoxic, biochemical and histopathological alterations in rats ", The Egyptian Journal of Hospital Medicine, vol. 15, pp. 40-56, 2004.
Mahmoud, A. M., " Pathological Study on Myxosporean Disease in Cultured Nile Tilapia (Oreochromis niloticus) Reared in Sandy and Muddy ponds in Egypt", Egyt. J. Comp. Path. & Clinic. Path., vol. vol. 17, no.2, pp. 250- 261, 2004.
Mahmoud, A. M., N. M. Ezz-Eldien, and E. E. Elsayed, " Tissue Protozoa (Myxobolus dermatobia) from the Eye of Tilapia zillii in Egypt, ", The First International Conference of the Veterinary Research Division, NRC, Egypt, pp. 15-17 February , 2004.
Sekena, A. - A. H., and A. M. Mahmoud, "Mutagenic and Histopathological Effects of Ribavirin Drug on Mice ", J. Egypt. Vet.Med. Assoc., vol. 64, no.2, pp. 153-149, 2004.
Abd El Aziz, M. A., G. M. Kamel, and M. A. M, "Seasonal study, Histopathological and Treatment Trials on Saproligniosis in some Fish Farms", 1st Scientific Conference of Faculty of Veterinary Medicine . , , Moshtohor- Benha University- Egypt, 2004.
2003
Manal, A. A. E., A. M. Mahmoud, and M. A. Olfat, " Role of stress-inducing corticosteroid on the development of parasitosis in Orechromis niloticus ", J. Egypt. Vet. Med. Ass., vol. 63, no.4, pp. 133-153, 2003.
Sokkar, S. M., and A. M. Mahmoud, "Adrenocortical Carcinoma in a donkey", German Veterinary Journal, vol. 110, pp. 176- 177, 2003.
2002
Ezz El -Dien, N. M., and A. M. Mahmoud, "Neorhadinorhynchus aspinosum (Acanthocephala: Echinorhynchidae) as a new record among Red Sea fish in Egypt with histopathological studies", J. Egypt. Vet. Med. Ass., vol. 62, no.5, pp. 233-243, 2002. Abstract

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Ehab, E. E., N. M. Ezz-Eldien, and A. M. Mahmoud, "Nodular cap disease in the red oranda and red cap breeds of gold fish( Crassius auratus) associated with dermocystidium species", Bulletin of the European association of fish pathologists (EAFP), vol. 22, no.1, pp. 7-14, 2002.
Mekawy, M. M., M. A. Mahmoud, and A. M. Amal, "Toxicopathological Studies on Malathion to Determine the Optimum Dose level Used to Erradicate Lethocerus Niloticum Insect Predating Fish", 1st Scientific Conference of the Egyptian Aquaculture Society, 1st Scientific Conference of the Egyptian Aquaculture Society., El-Arish, North Sinai-Egypt, 2002.
2001
Ibrahim, M. M., A. I. Shaaban, and A. M. Mahmoud, "Eels vibriosis in Lake Brolos (Egypt)", J. Egypt. Vet. Med. Ass., vol. 61, no.2, pp. 133-142, 2001.
Hassan, M. A., A. M. Mahmoud, and M. M. Moustafa, "Epizootiological and pathological studies of trypanorhynchiasis among some marine fishes of the Arabian Gulf", J. Egypt. Vet. Med. Ass., vol. 61, no.3, pp. 9-24, 2001.
Karima, M. F., Z. M. Malak, M. A. Mahmoud, and S. M. Sohair, "Genetical studies on melanomas in xiphpphorus fish", Arab J. Biotech,, vol. vol. 4, no.2, pp. 217-228, 2001.
Sokkar, S. M., A. M. Mahmoud, and M. F. Karima, "Histopathological and ultrastrucrural studies on melanomas in xiphophorus fishes during hybridization", Bulletin of the European association of fish pathologists (EAFP), vol. 21, no.2., pp. 56-63, 2001.
Ibrahim, M. M., and A. M. Mahmoud, "Histopathological observations on oral skin papilloma of cultured O. niloticus at the eastern province of Saudi Arabia", Beni-Suef Vet. Med. J., vol. 11, no.2, pp. 765-771, 2001.