Virulence and Antibiotic Resistance Patterns of Extended-Spectrum Beta-Lactamase-Producing serovar Heidelberg Isolated from Broiler Chickens and Poultry Workers: A Potential Hazard.

Citation:
Elhariri, M., R. Elhelw, S. Selim, M. Ibrahim, D. Hamza, and E. Hamza, "Virulence and Antibiotic Resistance Patterns of Extended-Spectrum Beta-Lactamase-Producing serovar Heidelberg Isolated from Broiler Chickens and Poultry Workers: A Potential Hazard.", Foodborne pathogens and disease, 2019.

Abstract:

The current study investigated the emergence of multidrug-resistance (MDR), extended-spectrum beta-lactamase (ESBL)-producing serovar Heidelberg in broiler chickens and workers in poultry farms. A total of 33 . Heidelberg isolates were recovered; 24 from the broiler cloacal swabs and 9 from the farm workers. All the . Heidelberg isolates were tested for susceptibility to 11 antimicrobial agents and for the presence of resistance and virulence genes. MDR strains were found in 95.8% (23/24) and 88.8% (8/9) of the broiler and human isolates, respectively. Among the MDR strains, 66.6% of the broiler isolates and 55.5% of the human isolates were ESBL producing. The majority of broiler isolates showed resistance to ampicillin (100%) and ceftriaxone (91.6%), followed by ceftazidime and imipenem, (87.5%) and (75%). The resistance rate of the human isolates to those antibiotics were lower than the broiler isolates; ampicillin (88.8%), ceftriaxone (66.6%), ceftazidime (77.7%), and imipenem (66.6%). The resistance determinant genes found among the isolated strains was , , , , , , and . The most detected ESBL genes for broiler and human isolates were (63.7%) and (56.6%), followed by (48.5%), (39.4%), and (27.3%); whereas and were not detected. The finding of chromosomal and plasmid virulence genes revealed that the A (100%), , C, and (72.8%), C (66.7%), (63.6%), B (54.6%), and A and A (3.0%), while A and R were absent. An elevated rate of MDR Heidelberg in chickens is of potential great health risk. This signifies the role of the food of animal origin as a reservoir of MDR that can affect the human health.

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