Publications

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Zafer, M. M., M. E. M. Bastawisie, M. Wassef, A. F. A. Hussein, and M. A. Ramadan, "Epidemiological features of nosocomial Klebsiella pneumoniae: virulence and resistance determinants", Future Microbiology, vol. 17, issue 1, pp. 27-40, Submitted.
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Shalaby, M. M., R. Samir, F. A. - Z. M. Goma, and M. A. Rammadan, "Enhanced fusidic acid transdermal delivery achieved by newly isolated and optimized Bacillus cereus Keratinase", International Journal of Biological Macromolecules, vol. 30, pp. e00620, 2021.
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Reda, M. M., A. S. Yassin, M. A. Rashed, and M. A. Ramadan, "development and evaluation of a novel technique for improvement of tetanus toxoid preparation", new Egyptian journal of microbiology, vol. 56, 2020.
Ramadan, M. A., A. E. Shawkey, M. A. Rabeh, and A. O. Abdellatif, "Expression of P53, BAX, and BCL-2 in human malignant melanoma and squamous cell carcinoma cells after tea tree oil treatment in vitro", Cytotechnology, vol. 71, issue 1, pp. 461-473, 2019.
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Nelson, J., A. O. El-Gendy, M. S. Mansy, M. A. Ramadan, and R. K. Aziz, "The biosurfactants iturin, lichenysin and surfactin, from vaginally isolated lactobacilli, prevent biofilm formation by pathogenic Candida", FEMS Microbiology letters, vol. 367, 2020.
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and Mohammed A. Ramadan 1, 2, S. M. K. E. 1 A. Y. 2*A. 2 M. S., "POTENTIAL IN VITRO ANTI-HELICOBACTER ACTIVITY OF BACTERIOCIN AND BACTERIOCIN-LIKE COMPOUNDS PRODUCED BY LACTOBACILLI", journal of microbiology, biotechnology and food sciences, vol. 4, issue 2, pp. 160-163, 2014. jmbfs-0540-ramadan.pdf
and Mohamed A. Ramdan, A.M. Hashem, 2W. A. - S. E. 1 1 A. 1T., "Decolorization of Reactive Black 5 by Micrococcus luteus and Candida albicans in Wastewaters", World Applied Sciences Journal , vol. 32, issue 2, pp. 153-163, 2014.
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KASHEF, M. O. N. A. T., N. M. Saleh, N. H. Assar, and M. A. Ramadan, "The Antimicrobial Activity of Ciprofloxacin-Loaded Niosomes against Ciprofloxacin-Resistant and Biofilm-Forming Staphylococcus aureus", Infection and Drug Resistance, vol. 13, pp. 1619-1629, 2020.
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Ibrahim, K. A., O. M. N. E. Y. A. M. HELMY, M. O. N. A. T. KASHEF, T. R. Elkhamissy, and M. A. Ramadan, "Identification of Potential Drug Targets in Helicobacter pylori Using In Silico Subtractive Proteomics Approaches and Their Possible Inhibition through Drug Repurposing", Pathogens, vol. 9, pp. 747, 2020.
Ibrahim, K. A., O. M. N. E. Y. A. M. HELMY, M. O. N. A. T. KASHEF, T. R. Elkhamissy, and M. A. Ramadan, "Identification of Potential Drug Targets in Helicobacter pylori Using In Silico Subtractive Proteomics Approaches and Their Possible Inhibition through Drug Repurposing", pathogens, vol. 9, issue 747, pp. 9090747, 2020.
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Elkadi, O. A., R. Hassan, M. El anany, H. J. Byrne, and M. A. Ramadan, "Identification of Aspergillus species in human blood plasma by infrared spectroscopy and machine learning", Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol. 248, pp. 119259, 2020.
El-sayed, N. R., R. Samir, L. J. M. Abdel-Hafez, and M. A. Ramadan, "Olive Leaf Extract Modulates Quorum Sensing Genes and Biofilm Formation in Multi-Drug Resistant Pseudomonas aeruginosa", antibiotics, vol. 9, pp. 526, 2020.
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Amer, M. A., R. Wasfi, A. S. Attia, and M. A. Ramadan, "Indole Derivatives Obtained from Egyptian Enterobacter sp. Soil Isolates Exhibit Antivirulence Activities against Uropathogenic Proteus mirabilis", Antibiotics, vol. 10, issue 4, pp. 363, 2021.
Amer, M. A., R. Wasfi, A. S. Attia, and M. A. Ramadan, "Indole Derivatives Obtained from Egyptian sp. Soil Isolates Exhibit Antivirulence Activities against Uropathogenic .", Antibiotics (Basel, Switzerland), vol. 10, issue 4, 2021. Abstract

is a frequent cause of catheter associated urinary tract infections (CAUTIs). Several virulence factors contribute to its pathogenesis, but swarming motility, biofilm formation, and urease activity are considered the hallmarks. The increased prevalence in antibiotic resistance among uropathogens is alarming and requires searching for new treatment alternatives. With this in mind, our study aims to investigate antivirulence activity of indole derivatives against multidrug resistant isolates. Ethyl acetate (EtOAc) extracts from sp. (rhizobacterium), isolated from Egyptian soil samples were tested for their ability to antagonize the virulence capacity and biofilm activity of uropathogens. Extracts of two sp. isolates (coded Zch127 and Cbg70) showed the highest antivirulence activities against . The two promising rhizobacteria Zch127 and Cbg70 were isolated from soil surrounding: (Zucchini) and (Cabbage), respectively. Sub-minimum inhibitory concentrations (Sub-MICs) of the two extracts showed potent antibiofilm activity with significant biofilm reduction of ten clinical isolates (-value < 0.05) in a dose-dependent manner. Interestingly, the Zch127 extract showed anti-urease, anti-swarming and anti-swimming activity against the tested strains. Indole derivatives identified represented key components of indole pyruvate, indole acetamide pathways; involved in the synthesis of indole acetic acid. Additional compounds for indole acetonitrile pathway were detected in the Zch127 extract which showed higher antivirulence activity. Accordingly, the findings of the current study model the feasibility of using these extracts as promising antivirulence agent against the uropathogens and as potential therapy for treatment of urinary tract infections (UTIs).

A.Gamal, A., H. Y. Abbas, N. A. M. Abdelwahed, M. T.Kashef, K. Mahmoud, M. A.Esawy, and MohammedA.Ramadan, "Optimization strategy of Bacillus subtilis MT453867 levansucrase and evaluation of levan role in pancreatic cancer treatment", International Journal of Biological Macromolecules, vol. 182, pp. 1590-1601, 2021.