Latifa Mohamed

Citation:

Hasan, M., L. Abdelgawad, D. Sabry, and abdelgwadmarwa, The Effect of Biodentine and/or Laser 650nm Photobiomodulation on Human Dental Pulp Stem Cells Considering Cell Viability and Odontogenic Differentiation, , vol. 164, pp. 431, 2020/03/03.

Abstract:

Vital pulp therapy preserves the health of injured dental pulp tissues through improving cells viability and formation of reparative dentine. This study conducted to assess the odontogenic effects of Biodentine (BD) and 650nm laser Photobiomodulation (PBM) in dentine regeneration from human dental pulp stem cells (hDPSCs). Seventy-two hDPSCs well-plates were divided into following: Group1: control. Group2: treated by BD. Group3: treated by 650nm PBM with 1J/cm2. Group4: treated by both 650nm PBM with 1J/cm2and BD. Group5: treated by 650nm PBM with 2J/cm2. Group6: treated by both 650nm PBM with 2J/cm2and BD. Cell viability was measured by MTT assay for 4days. While odontogenic differentiation of hDPSCs was assessed by alkaline-phosphatase-activity (ALP), Alizarin red staining (ARS), western blot and Reverse-transcriptase-PCR (RT-PCR) for dentine-sialophosphoprotein (DSPP) and Runt-related-transcription-factor2 (RUNX2) at day 21. The results showed statistically significant increase in viability of all study groups against control (p-value <0.0001) at 96hours. Also showed significant differences in ALP activity between groups (p-value <0.0001) and in expression of DSPP and RUNX2 (P<0.001) compared to control. The combination of both BD and PBM 2J/cm2 showed the highest number of calcified nodules. Overall BD and PBM increase the hDPSCs odontogenic differentiation and the combination of both treatments showed better effect.

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