Real-time quantitative RT-PCR and detection of tumour cell dissemination in breast cancer patients: plasmid versus cell line dilutions.

Citation:
Ismail, S. M., W. Wynendaele, J. L. E. Aerts, R. Paridaens, L. Van Mellaert, J. Anné, R. Gaafar, N. Shakankiry, H. M. Khaled, M. R. Christiaens, et al., "Real-time quantitative RT-PCR and detection of tumour cell dissemination in breast cancer patients: plasmid versus cell line dilutions.", Annals of oncology : official journal of the European Society for Medical Oncology / ESMO, vol. 14, issue 8, pp. 1241-5, 2003 Aug.

Abstract:

BACKGROUND: We previously developed a real-time quantitative RT-PCR technique to detect breast carcinoma cells in peripheral blood (PB). The aim of the current study was to improve cytokeratin 19 (CK19) quantification using plasmid dilutions of cloned PCR fragments to obtain a more reliable and reproducible quantification of CK19 transcripts.

MATERIALS AND METHODS: PB samples of 14 stage IV breast cancer patients and 23 healthy controls were examined with RT-PCR using plasmid quantification.

RESULTS: Median CK19+ copy numbers of one and 11 were detected in the control group and stage IV breast cancer patients, respectively (Mann-Whitney, P

Notes:

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