@article {151368,
	title = {Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System.},
	journal = {Scientific reports},
	volume = {7},
	year = {2017},
	month = {2017 Aug 04},
	pages = {7301},
	abstract = {<p>The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88-100\%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p \&lt; 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7\%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.</p>
},
	issn = {2045-2322},
	doi = {10.1038/s41598-017-07223-7},
	author = {Khalil, Karim and Elayat, Medhat and Khalifa, Elsayed and Daghash, Samer and Elaswad, Ahmed and Miller, Michael and Abdelrahman, Hisham and Ye, Zhi and Odin, Ramjie and Drescher, David and Vo, Khoi and Gosh, Kamal and Bugg, William and Robinson, Dalton and Dunham, Rex}
}
