Hanan Merey

Citation:

Elmasry, M. S., W. S. Hassan, H. A. Merey, and I. M. Nour, "Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma", BMC Chemistry, vol. 17, issue 74, pp. 1-11, 2023.

Abstract:

A novel, sensitive, and green micellar UPLC method was proposed and validated for the simultaneous
determination of four hypoglycemic agents used in type II diabetes mellitus treatment namely, pioglitazone,
alogliptin, glimepiride, and vildagliptin. The developed UPLC method was successfully applied for quantitative
analysis of these drugs in bulk, in pharmaceutical formulations, and in spiked human plasma. Chromatographic
separation was carried out on a Kinetex® 1.7 μm XB-C18 100 Å (50 × 2.1 mm) column, using a degassed and filtered
mixture of (0.1 M SDS- 0.3% triethyl amine- 0.1% phosphoric acid (pH 6)) and n-propanol (85:15 v/v), at a flow rate
of 0.2 mL/min. The experimental conditions of the suggested method were well investigated and optimized. The
newly developed micellar UPLC method is capable of determining different dosage forms at the same time with
the same solvents, saving time and effort. The method was found to be efficiently applicable in spiked human
plasma and could be extended to study the pharmacokinetics of the cited drugs in real human plasma samples.
The greenness of the developed method was evaluated by applying the Eco-scale scoring tool, which verified the
excellent greenness of the analytical method.