Assistant professor of Biochemistry
- Extensive studies and high background in different research fields like Biochemistry, Molecular Biology, Genetic Engineering DNA technology and Protein Biochemistry.
- Able to plan and write grant proposal, design the experimental procedures required for the recommended project – Design DNA gene sequencing for sub-cloning in E. coli and Yeast- using all techniques of DNA and protein analysis (electrophoresis, western blotting, imaging, electroporation) – Genomic and Gene isolation - Gene cloning and sub cloning in cloning in expression vectors of different systems (Classical way, Gateway, Lic cloning) – Primers design - PCR – Restriction digestion- Plasmid DNA preparation (Mini and Maxi-preparation)-DNA/RNA purification –DNA ligation – Competent cells preparation - DNA transformation – DNA shuffling for improving of enzymes activities - Recombinant protein expression in different hosts and protein purification (Ni- affinity chromatography, Ion Exchange chromatography and gel filtration) (Akta purifiers) – Protein characterization by measuring activity assay calorimetrically – Protein 2ndry structure determination by chirascan – study the effect of mutation of certain gene on its structural function stability by thermal and chemical denaturing.
- Screening for novel bacterial strain used for Bioremediation and isolation of their responsible enzymes.
- Screening and isolation of natural compounds from plants used for treatment of diabetes and blood pressure (natural products), by Thin Layer Chromatography (TLC) and Column Chromatography.
- Synthesis and biological application of metal inorganic drug complexes