Analytical investigation of different mathematical approaches utilizing manipulation of ratio spectra.

Citation:
Osman, E. E. A., "Analytical investigation of different mathematical approaches utilizing manipulation of ratio spectra.", Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, vol. 188, pp. 469-477, 2018 Jan 05.

Abstract:

This work represents a comparative study of different approaches of manipulating ratio spectra, applied on a binary mixture of ciprofloxacin HCl and dexamethasone sodium phosphate co-formulated as ear drops. The proposed new spectrophotometric methods are: ratio difference spectrophotometric method (RDSM), amplitude center method (ACM), first derivative of the ratio spectra (1DD) and mean centering of ratio spectra (MCR). The proposed methods were checked using laboratory-prepared mixtures and were successfully applied for the analysis of pharmaceutical formulation containing the cited drugs. The proposed methods were validated according to the ICH guidelines. A comparative study was conducted between those methods regarding simplicity, limitations and sensitivity. The obtained results were statistically compared with those obtained from the reported HPLC method, showing no significant difference with respect to accuracy and precision.