Detection of tick blood parasites in Egypt using PCR assay II- Borrelia burgdorferi sensu lato

Citation:
Detection of tick blood parasites in Egypt using PCR assay II- Borrelia burgdorferi sensu lato, K., Fatma, M. Emtithal, M. Refaat, and E. Hala , Journal of the Egyptian Society of Parasitology, Volume 4, Issue 3, p.553-564, (2010)

Abstract:

The prevalence of Borrelia burgdorferi sensu lato (s.l.), the etiologic agent
of Lyme borrelosis (LB), was determined for the first time in Egypt by using
polymerase chain reaction (PCR). Questing 5243 hard and soft ticks were collected from animal farms throughout Giza Governorate. DNA from 500 individual tick species was extracted and PCR was performed. Primers verified
from the sequence of German strain Pko of Borrelia afzelii were used. Fragments of 642 bp were generated and sequenced. The prevalence of B. burgdorferi sensu lato (s.l.) was 28 % of examined soft and hard ticks. High infection
rate (66%) of B. burgdorferi s.l. was observed in both nymph and adult soft
ticks Ornithodoros savignyi.
Beside, the role of hard ticks as potential vectors of Lyme disease in Egypt,
where the infection rate was between 0.0-50.0%. Sequence analysis of PCR
product of Borrelia burgdorferi sensu lato shares high degree of similarity in
sequence compared to similar species in GenBank.