Comparative study between the epimastigote stage infecting Cephalopina titillator (Diptera: Oestridae) and Trypanosoma evansi infecting camels (Camelus dromedarius).

Citation:
Hilali, M., A. - A. Wahab, A. S. Z. -Maogood, and A. M. ElBehairy, "Comparative study between the epimastigote stage infecting Cephalopina titillator (Diptera: Oestridae) and Trypanosoma evansi infecting camels (Camelus dromedarius).", Egyptian Veterinary Medical Society of Parasitology Journal, vol. 6, pp. 1-11, 2010.

Abstract:

This study aimed to finding possible relationship between epimastigote stage infecting Cephalopina titillator and Trypanosoma evansi infecting camels in Egypt. Nine rats were assigned into three equal groups and used for experimental infection. Rats of group 1 were inoculated intraperitoneally with 0.5ml of camel blood infected with T. evansi. Group 2 was inoculated intraperitoneally with 0.5ml of the fluid content of 3rd stage larvae of C. titillator infected with epimastigote stage. The 3rd group was kept as non-infected control. Blood smears were obtained daily from each rat of the experimental and control rats. Rats in group 1 showed the parasites in their blood on the 2nd day post infection (DPI) until the end of experiment 1month post infection (P.I.). The rats of group 2 and 3 showed no trypomastigote in their blood for a period of 1 month post inoculation. Sera were obtained from 1st, 2nd and 3rd groups at weekly interval starting from 1st week for a period of 4 weeks. The sera were tested using test kit CATT/ T. evansi. The sera of rats in group1 showed positive results in CATT from the 1st to the 4th week post inoculation. The rats of group 2 were positive for CATT at 3rd week and 4th week PI. While rats in group3 were negative for CATT during the experiment. Genomic DNA from purified trypanosomes and the fluid of 3rd stage larvae of C. titillator was extracted and DNA subjected to PCR technique. Also, PCR products of the two samples were purified from gel and the insert was sequenced and analyzed. The DNA from epimastigote form of C. titillator and that of T. evansi were positive for T. evansi by PCR using specific primer at 227bp. The result of gene sequence showed that the region of gene 227bp was identical in T. evansi and epimastigote stage obtained from C. titillator.

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