Clinical and laboratory diagnosis of piroplasmids in naturally infected cattle in Egypt.

Citation:
Ibrahim, A. K., A. M. ElBehairy, M. K.A, and W. S. Awad, "Clinical and laboratory diagnosis of piroplasmids in naturally infected cattle in Egypt.", J.Egypt.vet.med.Assoc., vol. 69, issue 2, pp. 197-209, 2009.

Abstract:

The present study provides clinical and laboratory examination of 54 cattle for infection by Theileria and Babesia piroplasmids. Prevalence of the clinical signs revealed high percentage of the infected animals showed pale mucous membrane (42.59%) and fever (38.88%), while low percentage showed swelling of lymph nodes and hematuria (18.51%). PCR result discovered the majority of Babesia spp infection (26%), most of them infected by B. bigemina (17%). The percentages of positive animals for Theileria annulata were 13% of total 22% Theileria infection. The use of PCR resulted in significantly higher efficacy of detection of bovine piroplasmids compared to microscopical examination of blood smears and allowed the specific discrimination between pathogenic and non-pathogenic Theileria spp which cannot be accomplished by traditional diagnosis. ELISA revealed higher babesiosis and theileriosis infection percentage (39% and 28% respectively) than that of PCR. The hematological and biochemical changes are demonstrated in this study.