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Yousof, H. - A. S., M. M. Khater, S. H. El-Sayed, and A. A. El-Badry, "Diagnostic biomarkers in murine Cryptosporidiosis: dose- and age-related infection", Journal of Parasitic Diseases, pp. 1–6, 2017. AbstractWebsite

Increasing prevalence of Cryptosporidium raises the importance to explore different aspects of its infection. In the absence of reproducible in vitro culturing, animal model is the only experimental method to study Cryptosporidium. Our study evaluated Cryptosporidium infection using coproscopy, copro-antigen and copro-DNA for early detection of murine cryptosporidiosis. Hundred and forty albino mice (neonates and adult) were divided into two groups, control group received sterile PBS solution, and infected groups were inoculated with molecularly characterized Cryptosporidium parvum oocysts and further subdivided into three subgroups for infectious dose response detection. Mice fecal samples were collected every 4 h on the first day and then daily and examined for fecal oocysts, copro-antigen and copro-DNA. Four mice from each subgroup were killed at 12, 24 and 48 h post-infection (P-I), and their intestines were examined for cryptosporidial mucosal DNA. Cryptosporidium copro-antigen and copro-DNA were detected 4 and 8 h P-I in infected neonatal and adult mice, respectively, and intestinal mucosal DNA was detected after 12 h in both. Microscopy was able to detect oocysts 48 h P-I. Inoculated C. parvum oocysts were recovered in feces of infected mice without genotypic changes. Neonate mice showed higher susceptibility for cryptosporidial infection than adults without statistical differences for the given infectious doses. Both copro-immunoassay and copro-nPCR assays can early detect Cryptosporidium infection; however, nPCR was able to identify Cryptosporidium species, making nPCR a reliable biomarker for early detection in murine model.

Yosof, H. - A. S. A., S. H. El-Sayed, E. Elfar, and M. M. Khater, "REPURPOSING OF ANTI-MALARIAL SYNRIAMTM AND TESTING ITS EFFICACY AGAINST EGYPTIAN STRAIN OF SCHISTOSOMA HAEMATOBIUM", Journal of the Egyptian Society of Parasitology, vol. 50, issue 2, pp. 344 - 352, 2020.
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Taha, N. M., H. - A. S. A. Yousof, S. H. El-Sayed, undefined, and M. S. I. Negm, "Atorvastatin repurposing for the treatment of cryptosporidiosis in experimentally immunosuppressed mice", Experimental Parasitology , vol. 181 , pp. 57-69, 2017.
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NASR, D. I. N. A. S., H. E. B. A. T. - A. L. L. A. H. S. A. YOUSOF, K. E. V. I. N. TYLER, and A. El-Badry, "GIARDIA INTESTINALIS ASSEMBLAGES AMONG EGYPTIAN SYMPTOMATIC/ASYMPTOMATIC CASES IN CAIRO", Journal of the Egyptian Society of Parasitology, vol. 48, issue 2, pp. 465 – 474, 2018. 6._giardia_intestinalis_assemblages_among_egyptian_symptomatic-asymptomatic_cases_in_cairo.pdf
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FAHMY, M. E. N. N. A. T. E. L. - R. A. H. M. A. N. A., H. - A. S. A. Yousof, S. H. El-Sayed, and S. S. Mahmoud, "IMMUNO-HISTOCHEMICAL CHANGES TRIGGERED BY REPEATED CRYPTOSPORIDIUM INFECTION IN IMMUNOSUPPRESSED MICE", Journal of the Egyptian Society of Parasitology, vol. 48, issue 3, pp. 483 – 892, 2018. 7.immuno-histochemical_changes_triggered_by_repeated_cryptosporidium_infection_in_immunosuppressed_mice_final_copy.pdf
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El-Sayed, S. H., H. E. B. A. T. - A. L. L. A. H. S. A. YOUSOF, and M. O. N. A. M. M. KHATER, "ASSESSMENT OF THE EFFECTOR ANTIBODIES AGAINST SCHISTOMA MANSONI AND THEIR IMPACT ON MEFLOQUINE EFFICACY", Journal of the Egyptian Society of Parasitology, vol. 48, issue 1, 2018. 5._assessment_of_the_effector_antibodies_against_schistosoma_mansoni_and_their_impact_on_mefloquine_efficacy.pdf
El Akkad, D. M. H., N. S. M. El-Gebaly, H. - A. S. A. Yousof, and M. A. M. Ismail, "Electron Microscopic Alterations in Pediculus humanus capitis Exposed to Some Pediculicidal Plant Extracts", The Korean Journal of Parasitology, vol. 54, no. 4: Korean Society for Parasitology, pp. 527, 2016. Abstract
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Abou-Shady, O. M., S. S. Mohammed, S. A. M. A. R. S. A. Y. E. D. ATTIA, H. - A. S. Yusuf, and D. O. Helmy, "Therapeutic effect of mefloquine on Schistosoma mansoni in experimental infection in mice", Journal of Parasitic Diseases: Springer, pp. 1–9, 2014. Abstract
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Abou-Shady, O. M., S. S. Mohammed, S. A. M. A. R. S. A. Y. E. D. ATTIA, and undefined, "In vitro Effect of Mefloquine on Adult Schistosoma mansoni", Research Journal of Parasitology, vol. 10, no. 3, pp. 111–119, 2015. Abstract

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