Publications

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Submitted
, , , vol. 8, issue 1, pp. 75, Submitted. Abstract
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2022
Nasrallah, A. K., A. A. Kheder, M. A. Kord, A. S. Fouad, M. M. El-Mogy, and M. A. M. Atia, "", Horticulturae, 2022. Abstract
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Fouad, A. S., A. B. Hamed, W. M. Amer, and R. M. Hafez, "Barcoding of Some Plant Species Using the rbcL Gene in the Mediterranean Oolitic Sand Dunes West of Alexandria, Egypt", Egyptian Journal of Botany, vol. 62, issue 1: National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT), pp. 159-168, 2022. AbstractWebsite

IN the Mediterranean Basin, oolitic sand dunes extend along the northeastern coast of Africa between the Gulf of Gabes (Tunisia) and the Nile Delta (Egypt), attenuating coastal vulnerability to storms and saltwater intrusion and protecting inland infrastructure. Psammophytes are typical and distinctive plants for dune habitats; they are the primary drivers for dune growth and stabilization by trapping sand particles in their shoot and root systems. The Anthropocene fingerprint may create an environment favorable for nonpsammophyte invasion and subsequent alteration in sand dune habitat structure and functions. The authentication of sand dune-inhabiting species should be a priority for conservation or restoration measures concerning sand dune habitats. This study is the first rbcL-based DNA barcoding documentation of 20 plant species inhabiting the vulnerable Egyptian oolitic sand dune habitats in Burg El Arab area, west of Alexandria. Results highlighted the applicability of the rbcL locus sequence for species authentication and recognition of the examined plant taxa sharing the same habitat. The phylogenetic analysis of rbcL barcode polymorphism revealed current taxonomic relationships among the assigned species. Results added valuable information to the authentication of sand dune plants, providing the first rbcL barcodes for species growing in oolitic sand dune habitats in Egypt, six of which were submitted for the first time to GenBank.

2021
Fouad, A., A. E. HEGAZY, E. Azab, E. Khojah, and T. Kapiel, "Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes", Plants, vol. 10, no. 10, 2021. Abstractnano_silver_alkaloids.pdfWebsite

Global agricultural systems are under unprecedented pressures due to climate change. Advanced nano-engineering can help increase crop yields while ensuring sustainability. Nanotechnology improves agricultural productivity by boosting input efficiency and reducing waste. Alkaloids as one of the numerous secondary metabolites that serve variety of cellular functions essential for physiological processes. This study tests the competence of silver nanoparticles (AgNPs) in boosting alkaloids accumulation in Catharanthus roseus suspension cultures in relation to the expression of C. roseus Mitogen Activated Protein Kinase 3 (CrMPK3) and Strictosidine Synthase (STR) genes. Five concentrations (5, 10, 15, 20 and 25 mg·L−1) of AgNPs were utilized in addition to deionized water as control. Results reflected binary positive correlations among AgNPs concentration, oxidative stress indicated with increase in hydrogen peroxide and malondialdehyde contents, activities of ascorbate peroxidase and superoxide dismutase, expression of the regulatory gene CrMPK3 and the alkaloid biosynthetic gene STR as well as alkaloids accumulation. These correlations add to the growing evidence that AgNPs can trigger the accumulation of alkaloids in plant cells through a signaling pathway that involves hydrogen peroxide and MAPKs, leading to up-regulation of the biosynthetic genes, including STR gene.

Ahmed, H. S. I., A. Badr, H. H. El-Shazly, L. Watson, A. S. Fouad, and F. Y. Ellmouni, "Molecular Phylogeny of Trifolium L. Section Trifolium with Reference to Chromosome Number and Subsections Delimitation", Plants, vol. 10, no. 10, 2021. Abstracttrifolium_phylogeny.pdfWebsite

The genus Trifolium is one of the largest genera of the legume family Fabaceae with ca. 255 species. The genus is divided into eight sections; the section Trifolium is a major section of the genus, comprising 73 species mainly distributed in the Mediterranean region. We used nuclear ribosomal DNA internal transcribed spacer (ITS) and morphological variation to reconsider the delimitation and phylogenetic relationships of species in the section Trifolium with reference to chromosomal variations. Bayesian analysis of ITS data delimited the species as three clades based on the analysis of ITS sequence and informative indels in combination with morphological variation. The phylogeny of the species by different analyses methods does not support their current delimitation in 17 subsections. The basic chromosome number x = 8 is the number for the genus Trifolium, from which x = 7, 6 and 5 were derived through successive aneuploidy events. With reference to the distribution of these numbers in the species of the section Trifolium, species in clade III and clade II are more evolved than species in clade I.

2020
Fouad, A. S., and R. M. Hafez, "Effects of cobalt ions and cobalt nanoparticles on transient expression of gus gene in catharanthus roseus suspension cultures", Journal of Radiation Research and Applied Sciences , vol. 13, issue 1, pp. 765-775, 2020.
Hafez, R., and A. S. Fouad, "Mitigation of Genotoxic and Cytotoxic Effects of Silver Nanoparticles on Onion Root Tips using some Antioxidant Scavengers", Egyptian Journal of Botany, vol. 60, issue 1, pp. 133-145, 2020.
2019
Fouad, A. S., R. Hafez, and R. Hamdy, "Authentication of Cordia dentata Poir. Growing in Egypt using ISSR and DNA barcoding", Bioscience Research, vol. 16, issue 2, 2019.
Fouad, A. S., R. Hafez, and H. Hosni, "Authentication of Three Endemic Species of the Family Caryophyllaceae from Sinai Peninsula Using DNA Barcoding", Egyptian Journal of Botany, vol. 59, 2019.
Fouad, A. S., and R. M. Hafez, "In silico characterization of CDKB1 and its coding gene in some Oryza species", Bioscience Research, vol. 16, issue 2, pp. 1679-1690, 2019.
2018
Shanab, S. M. M., R. M. Hafez, and A. S. Fouad, "A review on algae and plants as potential source of arachidonic acid", Journal of Advanced Research, vol. 11, pp. 3-11, 2018. 2_arachidonic_acid_review.pdf