Publications

Export 60 results:
Sort by: Author Title [ Type  (Asc)] Year
Journal Article
Khorshied, M. M., M. El-Ghamrawy, M. E. Yassa, A. M. S. Tosson, and M. A. El-Hady, "Association between BCL11A, HSB1L-MYB, and XmnI γG-158 (C/T) gene polymorphism and hemoglobin F level in Egyptian sickle cell disease patients", Annals of Hematology, vol. 99, issue 10, pp. 2279-2288, 2020.
Khorshied, M. M., M. El-Ghamrawy, M. E. Yassa, A. M. S. Tosson, and M. A. El-Hady, "Association between BCL11A, HSB1L-MYB, and XmnI γG-158 (C/T) gene polymorphism and hemoglobin F level in Egyptian sickle cell disease patients", Annals of Hematology, vol. 99, issue 10, pp. 2279-2288, 2020.
H, F., K. M, S. I, Gouda H, N. A, A. N, M. HA, Z. HM, and M. SM., "The association between hepatitis C virus infection, genetic polymorphisms of oxidative stress genes and B-cell non-Hodgkin's lymphoma risk in Egypt.", Infect Genet Evol. , vol. 12, issue 6, pp. 1189–1194, 2012. AbstractCU-PDF

Hepatitis C virus (HCV) has been postulated to be an etiological agent for lymphoid malignancies. Polymorphisms in oxidative stress genes as; superoxide dismutase (SOD2), glutathione peroxidase (GPX1), catalase (CAT), myeloperoxidase (MPO) and nitric oxide synthase (NOS2) may influence non-Hodgkin’s lymphoma (NHL) risk. HCV screening and polymorphisms in these five genes coding for antioxidant enzymes were studied in 100 Egyptian patients with B cell-NHL and 100 controls to clarify the association between HCV infection, oxidative stress genes polymorphisms and B cell-NHL risk. A significantly higher prevalence of HCV infection was detected among NHL patients relative to controls and this carried a 14-fold increased NHL risk (odds ratio (OR) = 14.3, 95% confidence interval (CI) = 5.4–38.3, p < 0.0001). GPX1 and MPO genetic polymorphisms conveyed increase in B-NHL risk (OR = 3.3, 95% CI = 1.4–7.4, p = 0.004 and OR = 4.4, 95% CI = 1.3–14.2, p = 0.009 respectively). Further analyses stratified by HCV infection revealed that concomitant HCV infection and GPX1 gene polymorphism had a synergetic effect on NHL risk with an OR of 15 (95%CI = 2.2–69.6, p < 0.0001). In addition, combined HCV infection and MPO gene polymorphisms had a pronounced NHL risk (OR = 9.2, 95%CI = 2.5–33.9, p < 0.0001). SOD2, CAT and NOS2 genetic polymorphisms were not found to confer increased NHL risk. This study revealed that HCV infection is a risk factor for NHL in Egypt. Polymorphisms in GPX1 and MPO genes may influence NHL risk in HCV infected Egyptian patients. Larger scale studies are warranted to establish this genetic susceptibility for NHL.

Gouda, H. M., M. M. Khorshied, I. A. Shaheen, M. E. H. Sissy, and M. A. Mohsen, "Association between matrix metalloproteinase 2 (MMP2) promoter polymorphisms and the susceptibility to non-Hodgkin's lymphoma in Egyptians.", Ann Hematol. , vol. 93, issue 8, pp. 1313-8, 2014.
Zoheir, N. M., M. S. Hamdy, M. M. Khorshied, N. N. Abulata, M. E. Sobky, A. M. Saleh, and H. M. Khairy, "Association of C46T genetic polymorphism of coagulation factor XII with deep venous thrombosis: a cohort study on Egyptian patients", Comparative Clinical Pathology, vol. 22, issue 2, pp. 203–207, 2013.
Arnaout, H. H., M. M. Khorshied, O. R. M. Khorshid, and M. H. El-Nagdy, "Association of Caspase 8 and Caspase 10 Genetic Polymorphisms with B-cell Non Hodgkin’s Lymphoma in Egypt: A Case-Control Study", J Cancer Sci Ther , vol. 4, issue 9, pp. 249-253 , 2012. association_of_caspase_8_and_caspase_10-nhl-ours-2012pdf.pdf
and - Hanaa Hamed Arnaout1, Mervat Mamdooh Khorshied1, O. R. K. 2* M. H. E. - N. M., "Association of Caspase 8 and Caspase 10 Genetic Polymorphisms with B-cell Non Hodgkin’s Lymphoma in Egypt: A Case-Control Study.", J Cancer Sci Ther , vol. 4, issue 9, pp. 249-253 , 2012. AbstractCU-PDF

Background and purpose: Non-Hodgkin lymphomas are closely related diseases with distinctive morphologic, immunophenotypic, genetic, and clinical features. Genetic susceptibility studies of NHL are mandatory to identify at risk populations and to clarify important disease mechanisms. Caspase genes play a key role in regulation of apoptotic cell death, and dysregulation of this signaling pathway has been shown to participate in tumorigenesis. The current study aimed at defining the role of Caspase 8-D302H, Caspase 8-652 6N ins/del and Caspase 10-I522L genetic polymorphisms as risk factors for NHL and their possible role as genetic prognostic markers.

Methods: The present study included 100 Egyptian B-cell NHL patients and 100 healthy controls. Genotyping of the studied genes was performed by polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique. Data was analyzed using SPSS statistical package version 15.

Results: The study revealed that CASP8-D302H mutant genotypes were significantly higher in NHL patients when compared to the controls and conferred increased risk of NHL. For CASP8-652 6N ins/del and Casp10- I522L, there was no statistical difference in the distribution of the different genotypes between NHL cases and the controls. Furthermore, there were no statistical differences between NHL patients harboring the wild or mutant genotypes of the studied genes as regards their response to therapy.

Conclusions: CASP8-D302H genetic polymorphism represents a genetic risk factor for NHL in Egyptian population. Hopefully, better understanding of the functional consequences of caspase genes polymorphism would provide a foundation for future studies of the possible role of these genes in lymphomagenesis.

Mostafa, W. Z., M. F. Hussein, E. F. Aly, M. M. Khorshied, E. B. Eweis, A. M. Abdelmeged, M. S. El-Hawary, and V. G. Hafez, "Circulating T regulatory cells in patients with psoriasis with and without atherosclerosis: A pilot comparative study before and after photochemotherapy.", Photodermatology, photoimmunology & photomedicine, 2021.
Khorshied, M. M., W. A. Said, and H. M. Shaaban, "Clinical implication of nucleophosmin gene mutation and Flt-3 internal tandem duplication in a cohort of Egyptian AML patients", Comparative Clinical Pathology, vol. 22, issue 3, pp. 497–506, 2013.
Farawela, H. M., M. M. Khorshied, H. M. Zawam, N. M. Kassem, and H. A. Kassem, "The clinical relevance and prognostic significance of adenosine triphosphate ATP-binding cassette (ABCB5) and multidrug resistance (MDR1) genes expression in acute leukemia: an Egyptian study.", J Cancer Res Clin Oncol. , vol. 140, issue 8, pp. 1323-30, 2014.
Karaksy, S. E. M., N. E. M. Guindy, H. M. Gouda, M. M. Khorshied, I. A. Shaheen, R. A. E. Khalil, and N. Y. Ibrahim, "Clinical relevance of angiopoietin-1, angiopoietin-2, and their receptor Tie-2 expression in acute myeloid leukemia", Comp Clin Pathol, 2010. angio_1_-2_and_tie_2_in_aml-2010-ours.pdf
Karaksy, S. E. M., N. E. M. Guindy, H. M. Gouda, M. M. Khorshied, I. A. Shaheen, R. A. E. Khalil, and N. Y. Ib, "Clinical relevance of angiopoietin-1, angiopoietin-2, and their receptor Tie-2 expression in acute myeloid leukemia.", Comparative Clinical Pathology, vol. 21, issue 6, pp. 1171-1177, 2012. AbstractCU-PDF

The angiogenic-related factors: angiopoietin-1 and -2 and their receptor Tie-2 have wide-ranging effects on tumor behavior that includes angiogenesis and, inflammation. These multifaceted pathways present a potential target in developing novel inhibition strategies for cancer therapy. The present work aimed at detecting the prevalence of expression of: angiopoietin-1, angiopoietin-2, and their receptor Tie-2 in 56 Egyptian de novo acute myeloid leukemia (AML) patients by conventional RT-PCR to verify the prognostic impact of their expression on the response to induction chemotherapy. Thirty age- and sex-matched healthy volunteers were subjected to the same analysis as a control group. High expression of angiopoietin-1 (Ang-1) was detected in the patient group but not the control group. AML patients expressing angiopoietin-2 (Ang-2) either solely or in combination with high Ang-1 and/or Tie-2 showed unfavorable response to induction chemotherapy; either failed induction or death during induction. These data provide evidence that the alternation of angiopoietin balance in favor of Ang-2 may play a critical role in the pathophysiology of AML. Furthermore, positive pre-therapeutic expression of Ang-2 indicates valiable unfavorable prognostic marker in AML patients and may be used as a prognostic tool in the risk-adaptive management of AML.

M, E. L. - G. N., K. MM, Gouda H, S. IA, A. K. RE, S. M. Y, and A. L. - B. TN, "The Clinical Relevance of Cyclin A1 over Expression in Acute Myeloid Leukemia", The Egyptian Journal of Haematology , vol. 33, issue 4, pp. 362-369, 2008.
Khorshied, M. M., and H. M. Gouda, "Diagnostic values of CD64, C-reactive protein and procalcitonin in ventilator-associated pneumonia in adult trauma patients: a pilot study.", Clin Chem Lab Med., vol. pii: /j/cclm.ahead-of-print/cclm-2015-0656/cclm-2015-0656.xml. doi: 10.1515/cclm-2015-0656. [Epub ahead of print], 2016.
Khorshied, M., O. Ibrahim, A. Gad, and M. El-Ghamrawy, "The effect of interleukin-1β and interleukin-6 genetic polymorphisms on sickle cell disease course in childhood: an Egyptian study", Archives of Medical Science-Civilization Diseases , vol. 3, issue 1, pp. 57-63, 2018.
R, Y., A. - H. MRE, K. M, K. M, and F. A, "Effect of polymorphisms in IL-12B p40, IL-17A and IL-23 A/G genes on the response of psoriatic patients to narrowband UVB", Photodermatol Photoimmunol Photomed. , vol. 34, issue 5, pp. 347-349, 2018.
BM, E. - Z., E. S, B. D, Z. NS, Z. NS, S. R, S. MA, A. - H. D, G. H, H. R, et al., "Effect of Procedural-Related Variables on Melanocyte-Keratinocyte Suspension Transplantation in Nonsegmental Stable Vitiligo: A Clinical and Immunocytochemical Study.", Dermatol Surg., vol. 43, issue 2, pp. 226-235, 2017.
M., E. K., E. F. T., E. S. G., T. A., A. M., A. F. H., E. K. O., K. M., and A. R. S. Gouda H., "Effect of Uremia on polymorphnuclear leucocytes activity and apoptosis", The Egyptian Journal of internal Medicine, vol. 19, issue 1, pp. 57-69, 2007.
AA, M., I. AM, E. - M. MW, M. IM, K. MA, G. HM, and R. RM., "Ex vivo expansion of stem cells: defining optimum conditions using various cytokines", Lab Hematol., vol. 12, issue 2, pp. 86-93, 2006.
AA, M., I. AM, E. - M. MW, M. IM, K. MM, G. HM, and R. RM., "Ex vivo expansion of stem cells: defining optimum conditions using various cytokines", Lab Hematol., vol. 12, issue 2, pp. 86-93, 2006.
Mostafa, A., M. Khorshied, H. Gouda, and M. Sroor, "Expresasion and clinical implication of Cyclic adenosine monophosphate response element binding protein (CREB) in de novo AML. ", Egypt. J. Lab. Med. , vol. 20, 2008.
M, K., Gouda H, M. I. A, S. M, and A. L. - B. TN, "Expression and Clinical Implication of ZAP-70 in Acute Lymphoblastic Leukemia ", Journal of Medical Science , vol. 8, issue 5, pp. 461-468, 2008.
Tourism