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A, I., A. R. H, K. M, S. R, N. N, and K. O, "Tumor necrosis factor alpha-308 and Lymphotoxin alpha+252 genetic polymorphisms and the susceptibility to non-Hodgkin lymphoma in Egypt.", Leuk Res. , vol. 36, issue 6, pp. 694-698, 2012. AbstractCU-PDF

Genetic polymorphism within the regulatory regions of tumor necrosis factor-alpha (TNF-α) and Lymphotoxin-alpha (LT-α) may be involved in the development of lymphoid malignancies. The aim of the current study was to investigate the effect of TNFα−308 and LTα+252 genetic polymorphism on susceptibility to non-Hodgkin lymphoma (NHL) in Egypt. Genotyping of the studied genes by restriction fragment length polymorphism polymerase chain reaction was conducted on 84 NHL and 100 healthy controls and revealed that TNFα−308 homotype (AA) was significantly higher in NHL patients and conferred sixfold increased risk of NHL (OR = 5.9, 95%CI = 2.3–16.1). Moreover, TNFα/LTα high-producer haplotypes were significantly higher in NHL patients and conferred increased risk of NHL (OR = 4.59, 95%CI = 2.19–9.42).

Saleh, N. F., N. Nabil, D. A. Bassiouny, and M. M. Khorshied, "Tumor necrosis factor α promotor polymorphism and nonsegmental vitiligo: a molecular susceptibility marker in Egyptian women ", Journal of The Egyptian Women's Dermatologic Society, vol. 11, issue 2, pp. 109-112, 2014.
Rahman, H. A. A., M. M. Khorshied, O. M. Khorshid, and S. M. Mahgoub, "Toll-like receptor 2 and 9 genetic polymorphisms and the susceptibility to B cell Non-Hodgkin Lymphoma in Egypt.", Ann Hematol. , vol. 93, issue 11, pp. 1859-65, 2014.
Esmat, S., D. Bassiouny, M. A. Saleh, D. abd el halim, R. Hegazy, M. El Hawary, H. E. B. A. GAWDAT, H. Gouda, M. Khorshied, and N. E. S. R. I. N. SAMIR, "Studying the effect of adding growth factors to the autologous melanocyte keratinocyte suspension in segmental vitiligo.", Dermatologic therapy, vol. 33, issue 3, pp. e13368, 2020. Abstract

Addition of different growth factors to the medium used in autologous melanocyte-keratinocyte transplantation procedure (MKTP) was reported in the literature. The aim of the current study was comparison of response to MKTP in segmental vitiligo (SV) with and without adding growth factors to the suspension medium. Eighteen cases with SV were randomly divided into two groups. In group A: Ham F12 medium was used for suspension and in group B: 5 ng/mL recombinant basic fibroblast growth factor (bFGF) and 25 mg/500 mL 3'5' cyclic adenosine monophosphate (cAMP) were added to the medium. All cases received NB-UVB twice weekly for 24 weeks. The area of vitiligo lesions was measured before and after therapy by point-counting technique and complications were recorded. Excellent response (90%-100% repigmentation) occurred in 5/9 cases (56%) in group A and 7/9 cases (78%) in group B (with growth factors). A significant decrease in the area of treated lesions before and after therapy was found in both groups A and B (P = .0012 and .0004, respectively), however, a higher percentage of reduction in area of vitiligo was seen in group B cases (70% in group A vs 90% in group B; P value: .028). Marginal halo was seen in five cases in group A and six in group B. In conclusion addition of bFGF and cAMP to MKTP medium improved the results of the procedure. It could be considered if economically feasible.

Raafat, I. I., N. A. Azab, M. M. Khorshied, M. H. Yacoub, and L. A. Samy, "Signal transducer and activator of transcription 4 (STAT4) G/T gene polymorphism in Egyptian systemic lupus erythematosus female patients ", The Egyptian Rheumatologist , vol. 37, pp. 75-80, 2015.
B, E. - Z., B. D, H. R, G. H, S. S, K. MM, and S. MA, "Rituximab treatment in pemphigus vulgaris: effect on circulating Tregs.", Arch Dermatol Res. 2017, 2017.
BM, E. - Z., A. OA, Z. NS, A. - R. HM, B. DA, and K. MM, "PTPN22 gene polymorphism in Egyptian alopecia areata patients and its impact on response to diphencyprone immunotherapy.", Gene, vol. Epub ahead of print, 2013. Abstract

PTPN22 1858C>T gene polymorphism has been associated with several autoimmune disorders including alopecia areata. The aim of the current study was to investigate the effect of the inherited genetic polymorphism 1858C>T of PTPN22 gene on the predisposition to severe forms of alopecia areata and its effect on the response to DPC treatment. To achieve our aim, PTPN22 1858C>T genotyping was performed by PCR-based restricted fragment length polymorphism (PCR-RFLP) analysis. The study included 103 Egyptian patients with extensive alopecia areata treated by DPC. Hundred healthy age and sex matched blood donors were included in the current study as a control group. Results of genotyping showed that PTPN22 CT and TT mutant genotypes were significantly higher in AA patients compared to controls and conferred increase risk of AA (OR = 2.601, 95% CI = 1.081–6.255). Statistical comparison between AA patients with wild and mutant genotypes revealed that the duration of the illness was significantly longer in those harboring the mutant genotypes. Moreover, the association of other autoimmune diseases as atopy and diabetes mellitus was higher in patients with mutant genotypes. Furthermore, PTPN22 1858C>T genetic polymorphism did not affect the patients' response to DPC immunotherapy.

T, E., E. N, Gouda H, K. M, M. D, and O. D, "Prognostic implication of BAALC gene expression in de novo acute myeloid leukemia patients", Egyptian Journal of Internal Medicine, vol. Jan-April, 2009.
hala Shiba, M. M. Khorshied, H. M. Gouda, S. A. Mousa, and M. K. El-Ghamarawy, "Prevalence of Hepatitis C among Egyptian Children with Sickle Cell Disease and the Role of IL28b Gene Polymorphisms in Spontaneous Viral Clearance.", Mediterr J Hematol Infect Dis, vol. 1;8(1):e2016007, 2016. il-28-hcv-scd-egypt.pdf
Hamdy, M. S. E. D., H. M. Gouda, I. A. M. Shaheen, M. M. Khorshied, and R. H. Tomerak, "Prevalence of factor V Leiden (G1619A) and prothrombin gene (G20210A) mutation in Egyptian children with sickle cell disease", Comparative Clinical Pathology , vol. 22, issue 4, pp. 697-702, 2013.
Eyada, T. K., K. MM, M. A. K. E. Din, R. A. Zayed, A. Soliman, and D. O. Darwish, "PRAME and WT1 Genes expression in Chronic Myeloid Leukemia Patients: Clinical importance and future prospects", Pan Arab Journal of Oncology, vol. 1, issue 1, pp. 99-108, 2009.
Shaheen, H. A., M. Khorshied, M. A. El-Sayed, M. A., and M. A. Taha, "Post Stroke Infection Frequency and Immunosuppression Contribution", Egyptian Journal of Neurology, Psychiatry & Neurosurgery, vol. 49, issue 3, pp. 239-244, 2012.
Khorshied, M. M., H. M. Gouda, I. A. Shaheen, and T. A. N. Bolkeny, "The osteogenic differentiation potentials of umbilical cord blood haemato-poietic stem cells", Comparative Clinical Pathology, vol. 21, issue 4, pp. 441–447, 2012.
Khorshied MM, Said WA, S. H. A., "Nucleophosmin gene mutation in acute myeloid leukemia patients.", Saudi Med J. , vol. 31, issue 5, pp. 582-4., 2010.
Khorshied, M. M., I. A. Shaheen, R. A. E. Khalil, and R. E. Sheir, "Methylene tetrahydrofolate reductase (MTHFR) gene polymorphisms in chronic myeloid leukemia: an Egyptian study.", Med Oncol, vol. 31, issue 1, pp. 794, 2014.
Deen, M. A. K. E., M. M. Khorshied, Z. A. E. Sadani, Y. M. Amrousy, and N. M. Galal, "Mannose-binding lectin (MBL2) gene polymorphism in sickle cell anemia: an Egyptian study", Comparative Clinical Pathology , vol. 22, issue 3, pp. 387-394, 2013.
Abdel Rahman HA, Khorshied MM, E. H. H. K. O. M., "The link between genetic polymorphism of glutathione-S-transferases, GSTM1, and GSTT1 and diffuse large B-cell lymphoma in Egypt.", J Cancer Res Clin Oncol. , vol. 138, issue 8, pp. 1363-8., 2012.
Ghobrial, E. E., H. M. Marzouk, M. M. Khorshied, and M. G. Sayed, "Level of Interleukin 37 (IL-37) in Children with Systemic Lupus Erythematosus and Its Correlation with Disease Activity", Iranian Journal of Pediatrics 28 , vol. 28, issue 1, 2018.
Shaarawy, E., R. A. N. I. A. A. B. D. EL-HAY, nesrine samir, and M. Khorshied, "Lack of Association between Interleukin-1 β Gene Polymorphisms and Alopecia Areata; A Case-Control Study ", Med. J. Cairo Univ., vol. 84, issue 2, pp. 197-201, 2016. il1b-vitiligo-2016-ours.pdf
Shaheen, I., M. Khorshied, Rasha Abdel-Raouf, H. Gouda, D. Kamal, N. Abulata, R. Aboukhalil, and B. Meligy, "L-Selectin P213S and Integrin Alpha 2 C807T Genetic Polymorphisms in Pediatric Sickle Cell Disease Patients.", Journal of pediatric hematology/oncology, vol. 42, issue 8, pp. e707-e711, 2020. Abstract

Sickle cell disease (SCD) is an autosomal recessive hemoglobinopathy characterized by increased cellular adhesiveness. Vaso-occlusion (VOC) is the most prevalent disease complication of SCD that could be altered by genetic factors. L-Selectin and integrin alpha 2 (ITGA2) are 2 adhesion molecules linked to vasculopathy and inflammation. The current study aimed at detecting the prevalence of genetic variants of L-selectin and ITGA2 as possible molecular modulators and novel therapeutic targets in a cohort of pediatric SCD patients. Genotyping was performed by polymerase chain reaction restriction fragment length polymorphism technique for 100 SCD patients and 100 age and gender-matched unrelated healthy controls. The homomutant genotype of ITGA2 C807T was significantly higher in SCD patients compared with controls (P=0.001) and confirmed almost a 3-fold increased risk of moderate and severe attacks of VOC. There are significant adverse effects caused by the polymorphisms of ITGA2, and hence Egyptian SCD patients could benefit from the targeted therapies specifically against ITGA2 to ameliorate the severe course of the disease and improve the quality of life. However, further studies of genotypes and expression levels of these adhesion molecules during the attacks of VOC are recommended.

Khorshied, M. M., A. A. Zayed, and M. F. Hussein, "Inducible nitric oxide synthase promoter polymorphism: a molecular susceptibility marker for vitiligo in Egyptians.", Int J Dermatol. , vol. 54(6), pp. 675-9, 2015.