Ibrahim, N. S., M. M. Makhlouf, G. H. Shahin, M. K. E. Ghamrawy, and N. M. Hussein, "The impact of MCP1-2518A/G and CCR2-V64I genetic polymorphisms in Egyptian sickle cell disease patients", Experimental and Molecular Pathology, vol. 128, pp. 104834, 2022.
Makhlouf, M. M., M. A. Ayoub, and D. F. Mourad, "The risk and prognostic influence of caspase 9 promotor polymorphisms in Egyptian patients with acute myeloid leukemia", Journal of Hematopathology, vol. 15, pp. 131-140, 2022.
Mousa, S. M., M. M. Makhlouf, E. T. Mohammed, and H. M. Zawam, "The Influence of Gene Polymorphisms on the Risk and Clinical Outcome of Non-Hodgkin Lymphoma.", Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion, vol. 37, issue 4, pp. 549-554, 2021. Abstract

Polymorphisms in the gene are associated with various diseases and cancers including non-Hodgkin lymphoma (NHL). The aim of the study is to assess the impact of genetic polymorphisms [- 330 T/G (rs2069762) and + 114 T/G (rs2069763)] on the susceptibility and prognosis of NHL. Sixty patients with NHL as well as 60 age and sex matched healthy control subjects are included in this study. genotypes were determined by Polymerase Chain Reaction-Restriction Fragment length Polymorphism assay (PCR-RFLP). Our study revealed that both rs2069762 and rs2069763 gene polymorphisms are associated with increased risk of developing NHL; OR = 3.609 (95% CI = 1.527-8.417) and 4.142 (95% CI = 1.637-10.538) respectively. Moreover, the simultaneous presence of both polymorphisms is associated with about 6 fold increased risk of developing NHL. Also, rs2069762 and rs2069763 gene polymorphisms increase the risk of unfavorable prognosis with OR = 17.300 (95% CI = 3.392-87.725) and 10.424(95% CI = 1.870-58.413) respectively. These findings suggest that (rs2069762) and (rs2069763) gene polymorphisms could be involved in the development of NHL.

Makhlouf, M. M., E. R. Radwan, O. M. Khorshed, L. M. Fathi, and M. M. Elmasry, "CXC Chemokine Receptor Type 5 Gene Polymorphisms in a Cohort of Egyptian Patients with Diffuse Large B-Cell Lymphoma.", Pathobiology : journal of immunopathology, molecular and cellular biology, vol. 88, issue 3, pp. 211-217, 2021. Abstract

BACKGROUND: The chemokine receptor CXCR5 is selectively expressed on B cells; it is involved in lymphocyte homing and the development of normal lymphoid tissue. Its principle ligand is CXCL13 or B lymphocyte chemoattractant. Three polymorphisms in the CXCR5 gene, rs148351692 C/G, rs6421571 C/T, and rs78440425 G/A, have been identified.

OBJECTIVE: To assess the genetic polymorphisms of CXCR5 and evaluate their possible contribution to the susceptibility and response to therapy of diffuse large B-cell lymphoma (DLBCL).

PATIENTS AND METHODS: Fifty DLBCL (not otherwise specified) patients and 50 control subjects were included in this study. CXCR5 genotypes were determined by PCR-RFLP.

RESULTS: Our study revealed that the CXCR5 rs148351692 C/G and rs6421571 C/T gene polymorphisms are associated with an increased risk of developing DLBCL (OR 28.57 [95% CI 8.96-96.56] and 3.45 [1.67-11.83] respectively), while CXCR5 rs78440425 G/A showed no association with the risk of lymphoma. Moreover, the double and triple combined gene polymorphisms are associated with an increased risk of developing DLBCL of approximately 120-fold and 105-fold, respectively. CXCR5 gene polymorphisms had no significant impact on disease outcome or response to therapy.

CONCLUSIONS: CXCR5 gene polymorphisms could be considered a potential risk factor for the development of DLBCL.

Abbas, O. M., K. A. Khalifa, M. M. Makhlouf, N. F. Osman, W. M. Abdel Razek, and A. S. Atta, "Influence of esophageal variceal bleeding on iron status in chronic hepatitis C patients", European Journal of Gastroenterology & Hepatology, vol. 32, issue 5, pp. 616-622, 2020.
Makhlouf, M. M., S. G. Elwakil, and N. S. Ibrahim, "Molecular and serological assessment of parvovirus B-19 infection in Egyptian children with sickle cell disease", Journal of Microbiology, Immunology and Infection, vol. 50, issue 5, pp. 565-569, 2017.
Hamdy, M. S. A., Z. A. El-Saadany, M. M. Makhlouf, A. I. Salama, N. S. Ibrahim, and A. A. Gad, "TAp73 and ΔNp73 relative expression in Egyptian patients with lymphoid neoplasms.", Tumori Journal, vol. 103, issue 3, pp. 268-271, 2017.
Ghanem, L. Y., M. M. Nosseir, A. A. Lotfi, A. S. Mohamed, R. A. Ibrahim, M. H. Hassanein, E. Mansour, M. M. Makhlouf, Y. M. Fouad, and H. R. El-Khayat, "Hematopoietic stem cell mobilization into the peripheral circulation in patients with chronic liver diseases", Journal of Digestive Diseases, vol. 13, issue 11, pp. 571-578, 2012.
Elsaid, A., and M. M. Makhlouf, "Surgical Management of Spontaneous Pyogenic Spondylodiscitis: Clinical and Radiological Outcome", Egyptian Journal of Neurosurgery , vol. 30, issue 3, pp. 221-226, 2015.
Makhlouf, M. M., and S. M. Abdel Hamid, "Expression of IL4 (VNTR intron 3) and IL10 (-627) genes polymorphisms in childhood immune thrombocytopenic purpura.", Laboratory Medicine, vol. 45, issue 3, pp. 211-219, 2014.