@article {231249, title = {Sperm kinetics of Egyptian buffalo bulls () affected by the red laser postfreezing.}, journal = {Journal of advanced veterinary and animal research}, volume = {9}, year = {2022}, pages = {396-404}, abstract = {

OBJECTIVE: To improve the fertilizing ability of frozen buffalo semen using the more beneficial, accurate, and cheap technique of laser irradiation at specific wavelengths and exposure times.

MATERIALS AND METHODS: The red laser source (625 nm) was used in this study with 5 watts output power and for the irradiation of the semen samples for 5 min; the laser focus spot area was 1 cm.Thirty straws belonging to five buffalo bulls were used in this study.

RESULTS: The results show that total motility (\%) and progressive motility (\%) increased insignificantly after 5 min of exposure (73.8 {\textpm} 1.4 and 60.4 {\textpm} 1.1, respectively) compared to the control sample (70.9 {\textpm} 0.9 and 57.5 {\textpm} 1.7, respectively). All velocity parameters (velocity average path,velocity curved line, and velocity straight line {\textmu}m/sec) recorded a significant (p \< 0.05) increase in samples measured 5 min after exposure (52.3 {\textpm} 1.3, 83.5 {\textpm} 2.0, and 43.5 {\textpm} 1.2, respectively) compared to the untreated ones (47.1 {\textpm} 2.0, 76.3 {\textpm} 3.1, and 38.6 {\textpm} 1.9, respectively).

CONCLUSION: The application of the red laser light on buffalo semen postthawing resulted in a positive correlation with almost every motility parameter; it may be recommended to apply this technique pre-in vitro fertilization for embryo production of buffalo species.

}, author = {Sherif Mohamed Dessouki and Dalia Abd-El Rahman Ahmed and Fayed, Ayat Kassem} }