@article {184665,
	title = {Long term conjugated linoleic acid supplementation modestly improved growth performance but induced testicular tissue apoptosis and reduced sperm quality in male rabbit.},
	journal = {PloS one},
	volume = {15},
	year = {2020},
	pages = {e0226070},
	abstract = {<p>Conjugated linoleic acid (CLA) is known for its multiple benefits including improvement of growth, increasing lean mass, and anti-carcinogenic effects. However, when used in long-term supplementations CLA does not improve semen parameters in boar and bull and reduces fertility in Japanese quails. The content of unsaturated fatty acids in dietary lipids plays a significant role in spermatogenesis owning the high proportion of unsaturated fatty acids in plasma membrane of sperms. Whether CLA plays a role in testicular tissue and epididymal fat is still unknown. Therefore, in this study we hypothesize that long-term supplementation of equal proportion of CLA isomer mix (c9,t11-CLA and t10,c12- CLA) in rabbit bucks might alter male reproductive potentials. Twelve V-Line weaned male rabbits were used in 26 weeks trial, rabbits were individually raised and randomly allocated into three dietary groups. Control group (CON) received a basal diet, a group received 0.5\% CLA (CLA 0.5\%), and a group received 1\% CLA (CLA 1\%). Rabbits were euthanized at the end of the trial and several parameters were evaluated related to growth, semen quality, and testicular and epididymal tissue histopathology and transcriptome. The long-term supplementation of CLA increased feed intake by 5\% and body weight by 2-3\%. CLA 1\% decreased sperm progressive motility. In testicular tissue L-carnitine and α-tocopherol were decreased by CLA supplementation. In epididymal fat, CLA tended to decrease concentration of polyunsaturated fatty acids, the expression of SCD5 gene was upregulated by CLA 1\% and CASP3 gene was upregulated by CLA 0.5\%. Transcription of PPARG was downregulated by CLA. Feeding 1\% CLA also decreased testicular epithelial thickness. Long-term supplementation of CLA modestly enhanced male rabbit growth, but negatively impacted male reproduction, especially at high dose of CLA.</p>
},
	attachments = {https://scholar.cu.edu.eg/sites/default/files/alzahraa/files/journal.pone_.0226070_1.pdf},
	author = {Abdelatty, A. M. and Badr, O A M and Mohamed, S.A. and M.S.  Khattab and Dessouki, SHM and Farid, O A A and Elolimy, A A and O. G. Sakr and El Hady, MA and Mehesen, G and Bionaz, M}
}
