@article {14955,
	title = {A Simple Technique for Long-Term Preservation of Leptospires},
	journal = {Journal of Basic Microbiology. },
	volume = {53},
	year = {2013},
	pages = {299 {\textendash} 301},
	abstract = {<p>The viability of six serovars of Leptospira spp. was studied after long storage at -70{\textdegree}C. The bacteria were either preserved in Ellinghausen{\textendash}McCullough{\textendash}Johnson{\textendash}Harris (EMJH) liquid growth medium or in sheep blood added as a cryoprotectant. The viability of the strains was observed on a monthly basis by dark-ground microscopy over a period of 20 months at -70{\textdegree}C. Addition of sheep blood was not significantly advantageous, since leptospires that were stored in EMJH showed a slight increase in number after recovery. The results suggest a very simple and useful technique for long-term preservation of such Leptospira.</p>
},
	author = {Samir, A and M. Wasfy}
}
