THERAPEUTIC EFFECT OF METFORMIN ON THE SQUAMOUS CELL CARCINOMA CELL LINE.

Citation:
Aziz, A. M. T. A., and S. Hassan, "THERAPEUTIC EFFECT OF METFORMIN ON THE SQUAMOUS CELL CARCINOMA CELL LINE.", The Arab Journal of Laboratory Medicine, vol. 44, issue 2, pp. 503-513, 2019.

Abstract:

Background: Understanding the actions of metformin on cell cycle, and particularly its effect on mitochondrial functions and cycle regulators, is important in the context of interest in ‘repurposing’ the compound for possible applications as a chemotherapeutic and/or chemopreventive agent in oncology.
Aim: This research was conducted to study the effect of metformin drug on the proliferation of cells in squamous cell carcinoma cell line (HEp-2) and the effect of metformin on the expression of cyclin D1 and mitochondrial COX1 genes.
Material and methods: MTT assay was applied to assess the rate of proliferation of cells in squamous cell carcinoma cell line HEp-2 before and after application of metformin in concentrations 5, 10, and 20 mmol for a period of 24, 48, and 72 hours. We assessed the quantitative expression of two genes mitochondrial COX1 and cyclinD1 using RT- PCR amplification and statistical analysis were performed on.
Results: The results of the present study showed that metformin significantly decreased both the proliferation of cells and the expression of both genes in a dose and time dependent manner. The cells showed significant decreased proliferation, with a significant positive direct linear correlation between both genes over the whole period.
Conclusion: It was concluded from this study that metformin affects the viability of cells through cell cycle arrest and apoptosis, there was an increase in mitochondrial COX1 and cyclin D1 genes in cancer cells where metformin significantly decreased their expression and hence is strongly recommended as an effective anticancer agent.
Key words: Metformin, squamous carcinoma cells.