Occupational Exposure to Heavy Metal Initiate Carcinogenesis Though BRAF/KRAS Over Expression and DNA Methylation.

Citation:
Aziz, A. M. T. A., H. M. E. Zahed, S. H. Ahmed, and W. Fathy, "Occupational Exposure to Heavy Metal Initiate Carcinogenesis Though BRAF/KRAS Over Expression and DNA Methylation.", Journal of Chemical Health Risks , 2020.

Abstract:

Colorectal cancer (CRC) is a leading cause of death especially in industries worker.
The aim of this study: is to identify the role of heavy elements exposure on CRC DNA methylation.
Methods: The study was conducted on 25 CRC patients. Biopsies were taken by colonoscopy from malignant tissue and adjacent normal tissues for comparative assessment of BRAF/KRAS, methylated MLH1 and MGMT between the normal and malignant tissues by using real time PCR. In an attempt to identify wither heavy metal like Lead, Aluminum and Mn have a role in cancer development or not, we compared their levels in the serum of 25 CRC patients and 25 normal volunteers by using atomic absorption.
Results: The expressions of BRAF/KRAS, methylated MLH1 and MGMT were significantly higher in malignant tissues compared to normal tissues (p value<0.001). Additionally, the levels of lead and aluminum but not Mn were significantly higher in CRC patients compared to normal controls (p value<0.001). Lead and Aluminum were positively correlated with all studied parameters.
Conclusion: Heavy metals act as starting signals for carcinogenesis through DNA methylation.
Keywords: Colorectal cancer; Heavy metals; DNA methylation.